Transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae cultured in recirculating aquaculture systems with and without biofloc
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In the present study, quantitative real-time RT-PCR was used to monitor the transcriptional responses of seven key genes related to some innate immune pathways in shrimp postlarvae after being placed in a recirculating aquaculture system (RAS) with or without biofloc (BF or no-BF). Each system consisted of the main tank with 400 L of seawater and six glass aquariums (50 L each). Besides, the nucleotide sequences of myosin light chain (LvMyo) from Litopenaeus vannamei related to the phagocytosis pathway were described. The sequence analysis indicated that LvMyo is a conserved protein among crustaceans and is present in other arthropods. The transcriptional response to the treatments showed several expression patterns. The prophenoloxidase gene was up-regulated in both systems (P < 0.05) and was higher in BF than in no-BF (P < 0.01). Phagocytosis-related genes depicted differential expressions. LvMyo, Ras-associated binding 6, and Ras-related nuclear protein expressions were higher in BF than in no-BF (P < 0.05). Regarding antioxidant genes, glutathione peroxidase was up-regulated only in BF (P < 0.05). Superoxide dismutase expression was lower in BF at 12 h (P < 0.05), but higher at 24 h (P < 0.05). These findings suggest that biofloc modulates the transcription of genes related to the immune response in shrimp as an early response or at the mid-term. Besides, the biological filter in a RAS without biofloc seems to be able to maintain a bacterial population that promotes a lower but similar response to that induced in the biofloc system.
KeywordsBiofloc Gene expression Glutathione peroxidase Heterotrophic culture Phagocytosis Prophenoloxidase RAS Superoxide dismutase Shrimp immune system
Recirculating aquaculture system
Treatment with biofloc
Treatment without biofloc
Litopenaeus vannamei myosine light chain
Ras-associated binding 6
Ras-related nuclear protein
We thank CONACyT for the scholarship (166929) to Jorge Soto Alcalá.
This study was funded by the Instituto Politécnico Nacional (SIP20151682 and SIP20160089).
Compliance with ethical standards
This study complies with the Mexican official Standard NOM-0062-ZOO-1999, technical specifications for the production, care and use of laboratory animals.
Conflict of interest
The authors declare that they have no conflict of interest.
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