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Aquaculture International

, Volume 27, Issue 1, pp 53–62 | Cite as

Effect of dietary administration of kappa carrageenan extracted from Hypnea musciformis on innate immune response, growth, and survival of Nile tilapia (Oreochromis niloticus)

  • L. VillamilEmail author
  • S. Infante Villamil
  • G. Rozo
  • J. Rojas
Article
  • 140 Downloads

Abstract

Immune stimulants are an alternative to antibiotic use and contribute to disease prevention in aquaculture. The effect of dietary administration of kappa carrageenan (Kc), extracted from the red algae Hypnea musciformis, in Nile tilapia (Oreochromis niloticus) was assessed by monitoring weight gain after a 15-day feeding trial. Immunostimulation was inferred by the relative expression of transferrin, interleukin 1β (IL-1β), and growth hormone (GH) in the spleen after 24 h and 15 days of daily administration. The toxic activity of Kc was evaluated in brine shrimp (Artemia salina) nauplii. No significant toxic effects of Kc were observed in A. salina at any dose studied. A positive tendency in growth rate and fish survival values was observed when Kc was administered. Correspondingly, GH, transferrin and IL-1 β levels at day 15 post-treatment were higher in the spleens of fish fed with Kc at 5 g kg−1 relative to non-Kc-treated control fish. Feeding Kc extract from H. musciformis to the fish improved nonspecific immunity parameters and increased survival and growth, but further research, including longer-termed studies, should be conducted before recommendation of Kc supplementation in tilapia diets at commercial scale.

Keywords

Immune-stimulant Oreochromis niloticus Hypnea musciformis Kappa carrageenan Edwardsiella tarda GH IL1β Transferrin 

Notes

Acknowledgements

The authors acknowledge the Jorge Tadeo Lozano University, Santa Marta Campus, Santa Marta, Colombia, for providing the funding to carry out these experiments (grant: Effect of native bacteria and kappa carrageenan as immune modulators for Nile tilapia) and the members of GICMOA. We would also like to recognize the support of La Sabana University for data analysis. The authors acknowledge Dr. Michael Hume, Research Biologist, United States Department of Agriculture, Agricultural Research Service, for the contributions in the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statements

Experiments were carried out according to the Laboratory Safety Manual of the participating institutions, Law 84 (National Congress of Colombia, 1989) and National Institutes of Health guide for the care and use of laboratory animals (NIH Publications No. 8023, revised 1978).

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • L. Villamil
    • 1
    Email author
  • S. Infante Villamil
    • 2
  • G. Rozo
    • 3
  • J. Rojas
    • 2
  1. 1.Facultad de IngenieríaUniversidad de La SabanaChiaColombia
  2. 2.Grupo de Investigación en Cultivo y Manejo de Organismos Acuáticos, Facultad de Ciencias Naturales e IngenieríaUniversidad Jorge Tadeo LozanoSanta MartaColombia
  3. 3.Grupo de Bioprospección y Biotecnología, Facultad de Ciencias Naturales e IngenieríaUniversidad Jorge Tadeo LozanoBogotáColombia

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