Advertisement

Aquaculture International

, Volume 26, Issue 3, pp 843–855 | Cite as

Dietary supplementation of yeast (Saccharomyces cerevisiae) improves growth, stress tolerance, and disease resistance in juvenile Nile tilapia (Oreochromis niloticus)

  • David Attim Abass
  • Kwasi Adu ObirikorangEmail author
  • Benjamin Betey Campion
  • Regina Esi Edziyie
  • Peter Vilhelm Skov
Article

Abstract

The yeast Saccharomyces cerevisiae is one of the commonest probiotics incorporated in aquafeeds. An 84-day feeding trial was conducted to evaluate the effects of varying dietary inclusions of S. cerevisiae, 0% (control), 3% (YF3), 5% (YF5), and 7% (YF7), on growth, stress tolerance, and disease resistance in juvenile (body mass ~ 21 g) Nile tilapia (Oreochromis niloticus). Fish were randomly distributed in groups of 20 into 12 1-m3 hapas and fed isoenergetic (~ 17 kJ g−1 gross energy) and isonitrogenous (~ 300 g kg−1 crude protein) diets at 3% of their bulk weight daily. Specific growth rates were significantly higher for the yeast-fed fish (0.77–0.78% day−1) than for the control fish (0.60% day−1) and resulted in significantly higher mean final weights for the yeast-treated groups. Protein and lipid retention efficiencies were also significantly higher in the yeast-fed fish than in the control group. In subsequent stress challenge trials, the yeast-fed fish had greater tolerance to acute heat as well as hypoxia exposure than the control fish. Survival rates of the yeast-treated groups following sudden exposure to elevated water temperature (40 °C) ranged from 82.5 to 100% compared to 15% for the control. Mean survival rates following a 24-h hypoxia exposure were also generally significantly higher for the yeast-fed fish. The probiotic groups recorded a relative percent survival (RPS) of 75% after a 14-day Aeromonas hydrophila infection challenge. The results of this study indicate that S. cerevisiae as an additive in Nile tilapia diets has beneficial impacts on growth, stress tolerance, and disease resistance.

Keywords

Aeromonas hydrophila Baker’s yeast Disease resistance Fish growth Oreochromis niloticus Probiotic 

Notes

Acknowledgements

The authors would like to thank the technical and laboratory staff of the Faculty of Renewable Natural Resources and the Faculty of Pharmacy and Pharmaceutical Sciences of the Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, for their assistance with the experimentation, laboratory analyses and Aeromonas hydrophila culture.

References

  1. Abdelghany AE (1998) Feed efficiency, nutrient retention and body composition of Nile tilapia, Oreochromis niloticus L., fed diets containing L-ascorbic acid, L-ascorbyl-2-sulphate or L-ascorbyl-2-polyphosphate. Aquac Res 29:503–510CrossRefGoogle Scholar
  2. Abu-Elala N, Marzouk M, Moustafa M (2013) Use of different Saccharomyces cerevisiae biotic forms as immune-modulator and growth promoter for Oreochromis niloticus challenged with some fish pathogens. Int J Vet Sci Med 1:21–29CrossRefGoogle Scholar
  3. Akhter N, Wu B, Memon AM, Mohsin M (2015) Probiotics and prebiotics associated with aquaculture: a review. Fish Shellfish Immunol 45:733–741CrossRefPubMedGoogle Scholar
  4. Amend DF (1981) Potency testing of fish vaccines. In: Anderson DP, Hennessen W (eds) Fish biologics: serodiagnostics and vaccines. Dev Biol Stand, vol 49. S. Karger AG, Basel, pp 447–454Google Scholar
  5. Association of official analytical chemists (AOAC) (2005) Official Methods of Analysis of the Official Association of Analytical Chemists, 18th edn. Association of Official Analytical Chemists, Arlington, VA, USAGoogle Scholar
  6. Azaare L (2017) Dissolved oxygen dynamics in selected aquaculture ponds in Kumasi. Dissertation, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaGoogle Scholar
  7. Balcázar JL, de Blas I, Ruiz-Zarzuela I, Cunningham D, Vendrell D, Múzquiz JL (2006) The role of probiotics in aquaculture. Vet Microbiol 114:173–186CrossRefPubMedGoogle Scholar
  8. Banerjee G, Ray AK (2017) The advancement of probiotics research and its application in fish farming industries. Res Vet Sci 115:66–77CrossRefPubMedGoogle Scholar
  9. Bligh EG, Dyer WJ (1959) A rapid method for total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefPubMedGoogle Scholar
  10. Burr G, Gatlin D, Ricke S (2005) Microbial ecology of the gastrointestinal tract of fish and the potential application of prebiotics and probiotics in finfish aquaculture. J World Aquacult Soc 36:425–436CrossRefGoogle Scholar
  11. Chiu C-H, Cheng C-H, Gua W-R, Guu Y-K, Cheng W (2010) Dietary administration of the probiotic, Saccharomyces cerevisiae P13, enhanced the growth, innate immune responses, and disease resistance of the grouper, Epinephelus coioides. Fish Shellfish Immunol 29:1053–1059CrossRefPubMedGoogle Scholar
  12. Cuesta A, Meseguer J, Esteban MA (2004) Total serum immunoglobulin M levels are affected by immunomodulators in seabream (Sparus aurata L.) Vet Immunol Immunopathol 101:203–210CrossRefPubMedGoogle Scholar
  13. Dimitroglou A, Davies S, Sweetman J (2008) The effects of dietary mannan oligosaccharides on the intestinal histology of rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol A 150:63CrossRefGoogle Scholar
  14. Egna HS, Boyd CE (1997) Dynamics of pond aquaculture. CRC Press, Boca RatonGoogle Scholar
  15. El-Boshy ME, El-Ashram AM, AbdelHamid FM, Gadalla HA (2010) Immunomodulatory effect of dietary Saccharomyces cerevisiae, β-glucan and laminaran in mercuric chloride treated Nile tilapia (Oreochromis niloticus) and experimentally infected with Aeromonas hydrophila. Fish Shellfish Immunol 28:802–808CrossRefPubMedGoogle Scholar
  16. Esteban MA, Rodríguez A, Meseguer J (2004) Glucan receptor but not mannose receptor is involved in the phagocytosis of Saccharomyces cerevisiae by seabream (Sparus aurata L.) blood leucocytes. Fish Shellfish Immunol 16:447–451CrossRefPubMedGoogle Scholar
  17. Gatesoupe FJ (1999) The use of probiotics in aquaculture. Aquacult 180:147–165CrossRefGoogle Scholar
  18. Gram L, Ringø E (2005) Prospects of fish probiotics. In: Holzapfel WH, Naughton PJ, Pierzynowski SG, Zabielski R, Salek E (eds) Microbial ecology in growing animals. Elsevier, Edinburgh, pp 379–417CrossRefGoogle Scholar
  19. Hai SV (2015) The use of probiotics in aquaculture. J Appl Microbiol 119:917–935CrossRefPubMedGoogle Scholar
  20. Harikrishnan R, Kim MC, Kim JS, Balasundaram C, Heo MS (2011) Immunomodulatory effect of probiotics enriched diets on Uronema marinum infected olive flounder. Fish Shellfish Immunol 30:964–971CrossRefPubMedGoogle Scholar
  21. Kim DH, Austin B (2006) Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics. Fish Shellfish Immunol 21:513–524CrossRefPubMedGoogle Scholar
  22. Li P, Gatlin MD (2003) Evaluation of brewer’s yeast (Saccharomyces cerevisiae) as a feed supplement for hybrid striped bass (Morone chrysops×M. saxatilis). Aquacult 219:681–692CrossRefGoogle Scholar
  23. Li P, Gatlin DM (2004) Dietary brewer’s yeast and probiotic GroBiotic™ AE influence growth performance, immune responses and resistance of hybrid striped bass (Morone chrysops × M. saxatilis) to Streptococcus iniae infection. Aquacult 231:445–456CrossRefGoogle Scholar
  24. Li P, Gatlin D (2006) Nucleotide nutrition in fish: current knowledge and future applications. Aquacult 251:141–152CrossRefGoogle Scholar
  25. Li Y, Bordinhon AM, Davis DA, Zhang W, Zhu X (2013) Protein: energy ratio in practical diets for Nile tilapia Oreochromis niloticus. Aquacult Int 21:1109–1119CrossRefGoogle Scholar
  26. Lokesh J, Fernandes JMO, Korsnes K, Bergh Ø, Brinchmann MF, Kiron V (2012) Transcriptional regulation of cytokines in the intestine of Atlantic cod fed yeast derived mannan oligosaccharide or β-glucan and challenged with Vibrio anguillarum. Fish Shellfish Immunol 33:626–631CrossRefPubMedGoogle Scholar
  27. Luo L, Cai X, He C, Xue M, Wu X, Cao H (2009) Immune response, stress resistance and bacterial challenge in juvenile rainbow trouts Oncorhynchus mykiss, fed diets containing chitosan oligosaccharides. Curr Zool 55:416–422Google Scholar
  28. Mora C, Maya MF (2006) Effects of the rate of temperature increase of the dynamic method on the heat tolerance of fish. J Therm Biol 31:337–341CrossRefGoogle Scholar
  29. Nayak SK (2010) Probiotics and immunity: a fish perspective. Fish Shellfish Immunol 29:2–14CrossRefPubMedGoogle Scholar
  30. Noh SH, Han K, Won TH, Choi YJ (1994) Effect of antibiotics, enzyme, yeast culture and probiotics on the growth performance of Israeli carp. Korean. J Anim Sci 36:480–486Google Scholar
  31. NRC (2011) Nutrient requirements of fish. National Research Council, National Academy Press, Washington D.C.  https://doi.org/10.17226/13039 CrossRefGoogle Scholar
  32. Ortuño J, Cuesta A, Rodríguez A, Esteban MA, Meseguer J (2002) Oral administration of yeast, Saccharomyces cerevisiae, enhances the cellular innate immune response of gilthead seabream (Sparus aurata). Vet Immunol Immunopathol 85:41–50CrossRefPubMedGoogle Scholar
  33. Pooramini M, Kamali A, Hajimoradloo A, Alizadeh M, Ghorbani R (2009) Effect of using yeast (Saccharomyces cerevisiae) as probiotic on growth parameters, survival and carcass quality in rainbow trout Oncorhynchus mykiss fry. Int Aquat Res 1:39–44Google Scholar
  34. Rahiman KMM, Jesmi Y, Thomas AP, Hatha AAM (2010) Probiotic effect of Bacillus NL110 and Vibrio NE17 on the survival, growth performance and immune response of Macrobrachium rosenbergii (de Man). Aquac Res 41:120–134CrossRefGoogle Scholar
  35. Reyes-Becerril M, Tovar-Ramírez D, Ascencio-Valle F, Civera-Cerecedo R, Gracia-López V, Barbosa-Solomieu V (2008) Effects of dietary live yeast Debaryomyces hansenii on the immune and antioxidant system in juvenile leopard grouper Mycteroperca rosacea exposed to stress. Aquacult 280:39–44CrossRefGoogle Scholar
  36. Riche M, Garling Jr DL (2004) Effect of phytic acid on growth and nitrogen retention in tilapia Oreochromis niloticus L. Aquac Nutr 10:389–400CrossRefGoogle Scholar
  37. Ringø E (2004) Lactic acid bacteria in fish and fish farming. In: Salminen S, Ouwehand A, von Wrigth A (eds) Lactic acid bacteria. Marcel Dekker Inc, New York, pp 581–610Google Scholar
  38. Ringø E, Gatesoupe F-J (1998) Lactic acid bacteria in fish: a review. Aquacult 160:177–203CrossRefGoogle Scholar
  39. Ringø E, Olsen RE, Gifstad TØ, Dalmo RA, Amlund H, Hemre G-I, Bakke AM (2010) Prebiotics in aquaculture: a review. Aquac Nutr 16:117–136CrossRefGoogle Scholar
  40. Rodríguez A, Cuesta A, Ortuño J, Esteban MA, Meseguer J (2003) Immunostimulant properties of a cell wall-modified whole Saccharomyces cerevisiae strain administered by diet to seabream (Sparus aurata L.) Vet Immunol Immunopathol 96:183–192CrossRefPubMedGoogle Scholar
  41. Sáenz de Rodrigáñez MA, Díaz-Rosales P, Chabrillón M, Smidt H, Arijo S, León-Rubio JM, Alarcón FJ, Balebona MC, Moriñigo MA, Cara JB, Moyano FJ (2008) Effects of dietary administration of probiotics on growth and intestine function of juvenile Senegalese sole (Solea senegalensis, Kaup 1858). Aquac Nutr 15:177–185CrossRefGoogle Scholar
  42. Sajeevan TP, Philip R, Singh ISB (2009) Dose/frequency: a critical factor in the administration of glucan as immunostimulant to Indian white shrimp Fenneropenaeus indicus. Aquacult 287:248–252CrossRefGoogle Scholar
  43. Salnur S, Gultepe N, Hossu B (2009) Replacement of fish meal by yeast (Saccharomyces cerevisiae): effects on digestibility and blood parameters for Gilthead Sea bream (Sparus aurata). J Anim Vet Adv 8:2557–2561Google Scholar
  44. Siwicki AK, Anderson DP, Rumsey GL (1994) Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Vet Immunol Immunopathol 4:125–139CrossRefGoogle Scholar
  45. Smith VJ, Brown JH, Hauton C (2003) Immunostimulation in crustaceans: does it really protect against infection? Fish Shellfish Immunol 15:71–90CrossRefPubMedGoogle Scholar
  46. Sørum H (2006) Antimicrobial drug resistance in fish pathogens. In: Aarestrup FM (ed) Antimicrobial resistance in bacteria of animal origin. ASM Press, Washington DC, pp 213–238Google Scholar
  47. Taoka Y, Maeda H, Jo J-Y, Jeon M-J, Bai SC, Lee W-J, Yuge K, Koshio S (2006) Growth, stress tolerance and non-specific immune response of Japanese flounder, Paralichthys olivaceus to probiotics in a closed recirculating system. Fish Sci 72:310–321CrossRefGoogle Scholar
  48. Tukmechi A, Bandboni M (2014) Effects of Saccharomyces cerevisiae supplementation on immune response, hematological parameters, body composition and disease resistance in rainbow trout, Oncorhynchus mykiss (Walbaum, 1792). J Appl Ichthyol 30:55–61CrossRefGoogle Scholar
  49. Verschuere L, Rombaut G, Sorgeloos P, Verstraete W (2000) Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64:655–671CrossRefPubMedPubMedCentralGoogle Scholar
  50. Vijayan KK, Singh ISB, Jayaprakash NS, Alavandi SV, Pai SS, Preetha R, Rajan JJS, Santiago TC (2006) A brackish water isolate of Pseudomonas PS-102, a potential antagonistic bacterium against pathogenic vibrios in penaeid and non-penaeid rearing systems. Aquacult 251:192–200CrossRefGoogle Scholar
  51. Viola S, Arieli Y, Zohar G (1988) Animal-protein-free feeds for hybrid tilapia (Oreochromis niloticus × O. aureus) in intensive culture. Aquacult 75:115–125CrossRefGoogle Scholar
  52. Zar JH (1999) Biostatistical analysis, 4th edn. Prentice Hall, New JerseyGoogle Scholar
  53. Zorriehzahra MJ, Delshad ST, Adel M, Tiwari R, Karthik K, Dhama K, Lazado CC (2016) Probiotics as beneficial microbes in aquaculture: an update on their multiple modes of action: a review. Vet Q 36:228–241CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • David Attim Abass
    • 1
  • Kwasi Adu Obirikorang
    • 1
    Email author
  • Benjamin Betey Campion
    • 1
  • Regina Esi Edziyie
    • 1
  • Peter Vilhelm Skov
    • 2
  1. 1.Department of Fisheries and Watershed Mangement, Faculty of Renewable Natural ResourcesKwame Nkrumah University of Science and TechnologyKumasiGhana
  2. 2.DTU Aqua, Section for Aquaculture, The North Sea Research CentreTechnical University of DenmarkHirtshalsDenmark

Personalised recommendations