Aquaculture International

, Volume 24, Issue 1, pp 127–144 | Cite as

Dietary supplementation with mannan oligosaccharide influences growth, digestive enzymes, gut morphology, and microbiota in juvenile striped catfish, Pangasianodon hypophthalmus

  • Mst. Nahid Akter
  • Amalia Sutriana
  • Allah Dad Talpur
  • Roshada Hashim
Article

Abstract

This study was conducted to evaluate the effects of mannan oligosaccharide (MOS) dietary supplementation on growth, digestive enzyme activities, gut morphology, and microbiota in striped catfish (Pangasianodon hypophthalmus). Triplicate groups of juvenile striped catfish (initial weight 20.41 ± 1.64 g) were fed twice per day at 2.5 % of body weight for 12 weeks, with 0 (control), 0.2, 0.4, 0.6, or 0.8 % MOS diets. Compared to control (0.74 ± 0.03) and fish fed low MOS concentration, those fed 0.6 % (1.01 ± 0.02) or 0.8 % (1.03 ± 0.03) MOS had significantly higher (P < 0.05) specific growth rates. Feed conversion ratio and protein efficiency ratio, respectively, significantly improved (P < 0.05) in fish fed 0.6 % (1.62 ± 0.23; 1.71 ± 0.23) or 0.8 % (1.42 ± 0.06; 1.92 ± 0.08) MOS. Further, compared to control and fish fed 0.2 % MOS, those fed 0.6 % MOS had significantly higher (P < 0.05) apparent protein and dry matter digestibilities. Amylase, protease, and lipase activities, respectively, were significantly higher (P < 0.05) in fish fed 0.6 % MOS (5.10 ± 0.97; 0.47 ± 0.08; and 5.59 ± 1.82) than in control (2.60 ± 0.53; 0.32 ± 0.05; and 3.78 ± 0.72). Histological analysis of the anterior and posterior gut showed significantly higher (P < 0.05) villus length in 0.6 and 0.8 % MOS fed fish than in the control and other treatments. The guts of fish fed different MOS concentrations showed similar total lactic acid bacteria counts, which were significantly higher (P < 0.05) than those in the control. Thus, 0.6 % MOS-supplemented diet improved growth, feed utilization, digestive enzyme activities, gut morphology, and microbiota in juvenile striped catfish.

Keywords

Digestive enzyme activities Growth performance Gut microbiota Intestinal structure Nutrient digestibility Pangasianodon hypophthalmus Prebiotics 

Abbreviations

MOS

Mannan oligosaccharide

CMC

Carboxymethyl cellulose

NFE

Nitrogen-free extract

GE

Gross energy

HSI

Hepatosomatic index

IPF

Intraperitoneal fat

VSI

Viscerosomatic index

SGR

Specific growth rate

FCR

Food conversion ratio

PER

Protein efficiency ratio

AOAC

Association of official analytical chemists

PVA

Polyvinyl alcohol

TEM

Transmission electron microscope

LAB

Lactic acid bacteria

TB

Total bacteria

APD

Apparent protein digestibility

ADM

Apparent dry matter digestibility

CFU

Colony-forming unit

MRS

De Man, Rogosa, and Sharpe

TSA

Tryptic soy agar

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Aquaculture Research Group, Laboratory of Feeds and Feeding Management, School of Biological SciencesUniversiti Sains MalaysiaPenangMalaysia
  2. 2.Faculty of FisheriesHajee Mohammad Danesh Science and Technology UniversityDinajpurBangladesh
  3. 3.Faculty of Veterinary MedicineSyiah Kuala UniversityAcehIndonesia

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