Aquaculture International

, Volume 22, Issue 4, pp 1263–1282 | Cite as

Effect of oxidation–reduction potential on performance of European sea bass (Dicentrarchus labrax) in recirculating aquaculture systems

Article
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Abstract

The direct impact of oxidation–reduction potential (ORP) on fish welfare and water quality in marine recirculating aquaculture systems (RAS) is poorly documented. In this study, the effects of the fish size (S1, S2, S3) and ORP level (normal, four successive levels) on the performance of European sea bass (Dicentrarchus labrax) were investigated. Three size fish were distributed into two RAS (RAS and RAS O3). Ozone was injected into RAS O3 to increase the ORP level. The ORP was stabilized to four successive levels: 260–300, 300–320, 320–350, and 300–320 mV in fish tanks during four periods (P1–4). At the last day of each period, the hematological parameters, plasma protein and mortality of sea bass were analyzed. Two-way ANOVA revealed that several hematological parameters, including pH, hematocrit, concentrations of oxygen, carbon dioxide, glucose (Glu), ionized calcium, kalium, and hemoglobin, were significantly influenced by the increased ORP levels over the experimental period. The alteration in blood Glu and plasma protein concentration showed that ORP around 300–320 mV started to stress sea bass. Once the ORP exceeded 320 mV in the tanks during the P3 period, mortality occurred even when total residual oxidants/ozone-produced oxidants was only 0.03–0.05 mg L−1 in the fish tanks. At the same time, plasma protein decreased notably due to appetite depression. After the decrease in ORP during the P4 period, mortality continued. In conclusion, the results strongly suggest that for European sea bass in RAS, the ORP should not exceed 320 mV in the tanks. Once ozonation damaged fish, the effect seemed to be irreversible. However, how ORP affected related hematological parameters still need the further investigations.

Keywords

ORP Performance European sea bass RAS Ozone 
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Notes

Acknowledgments

The authors would thank all the participants from the Ifremer Palavas station: Cyrille Przybyla, Myriam Callier, and Thibault Geoffroy for their contribution to the experiment and analyses. This work was supported by the National Natural Science Foundation of China (Grant No. 41306152) and National Science and Technology Support Program (Grant No. 2011BAD13B04).

References

  1. Banhidi M (1995) pH and ORP. Met Finish 93:544–550CrossRefGoogle Scholar
  2. Blancheton JP (2000) Developments in recirculation systems for Mediterranean fish species. Aquac Eng 22:17–31CrossRefGoogle Scholar
  3. Buchan KAH, Martin-Robichaud DJ, Benfey TJ (2005) Measurement of dissolved ozone in sea water: a comparison of methods. Aquac Eng 33:225–231CrossRefGoogle Scholar
  4. Buchan KAH, Martin-Robichaud DJ, Benfey TJ, MacKinnon AM, Boston L (2006) The efficacy of ozonated seawater for surface disinfection of haddock (Melanogrammus aeglefinus) eggs against piscine nodavirus. Aquac Eng 35:102–107CrossRefGoogle Scholar
  5. Bullock GL, Summerfelt ST, Noble AC, Weber AL, Durant MD, Hankins JA (1997) Ozonation of a recirculating rainbow trout culture system I. Effects on bacterial gill disease and heterotrophic bacteria. Aquaculture 158:43–55CrossRefGoogle Scholar
  6. Davidson J, Good C, Welsh C, Summerfelt S (2011) The effects of ozone and water exchange rates on water quality and rainbow trout Oncorhynchus mykiss performance in replicated water recirculating systems. Aquac Eng 44:80–96CrossRefGoogle Scholar
  7. Endo H, Yonemori Y, Musiya K, Maita M, Shibuya T, Ren H, Hayashi T, Mitsubayashi K (2006) A needle-type optical enzyme sensor system for determining glucose levels in fish blood. Anal Chim Acta 22:573–574Google Scholar
  8. Forneris G, Bellardi S, Palmegiano GB, Saroglia M, Sicuro B, Gasco L, Zoccarato I (2003) The use of ozone in trout hatchery to reduce saprolegniasis incidence. Aquaculture 221:157–166CrossRefGoogle Scholar
  9. Fukunaga K, Suzuki T, Arita M, Suzuki S, Hara A, Yamauchi K, Shinriki N, Ishizaki K, Takama K (1992) Acute toxicity of ozone against morphology of gill and erythrocytes of Japanese charr (Salvelinus-Leucomaenis). Comp Biochem Phys C 101:331–336CrossRefGoogle Scholar
  10. Fukunaga K, Nakazono N, Suzuki T, Takama K (1999) Mechanism of oxidative damage to fish red blood cells by ozone. IUBMB Life 48:631–634PubMedCrossRefGoogle Scholar
  11. Good C, Davidson J, Welsh C, Snekvik K, Summerfelt S (2011) The effects of ozonation on performance, health and welfare of rainbow trout Oncorhynchus mykiss in low-exchange water recirculation aquaculture systems. Aquac Eng 44:97–102CrossRefGoogle Scholar
  12. Jensen MA, Ritar AJ, Burke C, Ward LR (2011) Seawater ozonation and formalin disinfection for the larval culture of eastern rock lobster, Jasus (Sagmariasus) verreauxi, phyllosoma. Aquaculture 318:213–222CrossRefGoogle Scholar
  13. Jiang G, Liu Y, Yang D, Lue Y (2001) The toxicity of ozonated seawater to the Penaeus chinensis and Paralichthys olivaceus. Mar Sci 25(3):11–13Google Scholar
  14. Kristensen T, Åtland Å, Rosten T, Urke HA, Rosseland BO (2009) Important influent-water quality parameters at freshwater production sites in two salmon producing countries. Aquac Eng 41:53–59CrossRefGoogle Scholar
  15. Liu XQ, Wang J, Zhang D, Li YT (2009) Grey relational analysis on the relation between marine environmental factors and oxidation–reduction potential. Chin J Oceanol Limnol 27:583–586CrossRefGoogle Scholar
  16. Maricchiolo G, Mirto S, Caruso G, Caruso T, Bonaventura R, Celi M, Matranga V, Genovese L (2011) Welfare status of cage farmed European sea bass (Dicentrarchus labrax): a comparison between submerged and surface cages. Aquaculture 314:173–181CrossRefGoogle Scholar
  17. Park J, Kim Y, Kim PK, Daniels HV (2011) Effects of two different ozone doses on seawater recirculating systems for black sea bream Acanthopagrus schlegeli (Bleeker): removal of solids and bacteria by foam fractionation. Aquac Eng 44:19–24CrossRefGoogle Scholar
  18. Reiser S, Schroeder JP, Wuertz S, Kloas W, Hanel R (2010) Histological and physiological alterations in juvenile turbot (Psetta maxima, L.) exposed to sublethal concentrations of ozone-produced oxidants in ozonated seawater. Aquaculture 307:157–164CrossRefGoogle Scholar
  19. Richardson LB, Burton DT, Block RM, Stavola AM (1983) Lethal and sublethal exposure and recovery effects of ozone-produced oxidants on adult white perch (Morone americana Gmelin). Water Res 17:205–213CrossRefGoogle Scholar
  20. Ritar AJ, Smith GG, Thomas CW (2006) Ozonation of seawater improves the survival of larval southern rock lobster, Jasus edwardsii, in culture from egg to juvenile. Aquaculture 261:1014–1025CrossRefGoogle Scholar
  21. Roque A, Yildiz HY, Carazo I, Duncan N (2010) Physiological stress responses of sea bass (Dicentrarchus labrax) to hydrogen peroxide (H2O2) exposure. Aquaculture 304:104–107CrossRefGoogle Scholar
  22. Sammouth S, d’Orbcastel ER, Gasset E, Lemarié G, Breuil G, Marino G, Coeurdacier JL, Fivelstad S, Blancheton JP (2009) The effect of density on sea bass (Dicentrarchus labrax) performance in a tank-based recirculating system. Aquac Eng 40:72–78CrossRefGoogle Scholar
  23. Sanni S, Forsberg OI (1996) Modelling pH and carbon dioxide in single-pass sea-water aquaculture systems. Aquac Eng 15:91–110CrossRefGoogle Scholar
  24. Saravanan M, Prabhu Kumar K, Ramesh M (2011) Haematological and biochemical responses of freshwater teleost fish Cyprinus carpio (Actinopterygii: cypriniformes) during acute and chronic sublethal exposure to lindane. Pestic Biochem Phys 100:206–211CrossRefGoogle Scholar
  25. Schroeder JP, Croot PL, Von Dewitz B, Waller U, Hanel R (2011) Potential and limitations of ozone for the removal of ammonia, nitrite, and yellow substances in marine recirculating aquaculture systems. Aquac Eng 45:35–41CrossRefGoogle Scholar
  26. Silva J, Laranjeira A, Serradeiro R, Santos MA, Pacheco M (2011) Ozonated seawater induces genotoxicity and hematological alterations in turbot (Scophthalmus maximus)—Implications for management of recirculation aquaculture systems. Aquaculture 318:180–184CrossRefGoogle Scholar
  27. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76–85PubMedCrossRefGoogle Scholar
  28. Soria G, Merino G, von Brand E (2007) Effect of increasing salinity on physiological response in juvenile scallops Argopecten purpuratus at two rearing temperatures. Aquaculture 270:451–463CrossRefGoogle Scholar
  29. Summerfelt ST, Hankins JA, Weber AL, Durant MD (1997) Ozonation of a recirculating rainbow trout culture system II. Effects on microscreen filtration and water quality. Aquaculture 158:57–67CrossRefGoogle Scholar
  30. Summerfelt ST, Sharrer MJ, Tsukuda SM, Gearheart M (2009) Process requirements for achieving full-flow disinfection of recirculating water using ozonation and UV irradiation. Aquac Eng 40:17–27CrossRefGoogle Scholar
  31. Tal Y, Schreier HJ, Sowers KR, Stubblefield JD, Place AR, Zohar Y (2009) Environmentally sustainable land-based marine aquaculture. Aquaculture 286:28–35CrossRefGoogle Scholar
  32. Tango MS, Gagnon GA (2003) Impact of ozonation on water quality in marine recirculation systems. Aquac Eng 29:125–137CrossRefGoogle Scholar
  33. Wang Y, Liu DX, Ju MT, Jin ZH, Li TL (2011) The effect of seawater salinity on the equilibrium time of oxidation reduction potential. Adv Mater Res 301:1648–1651CrossRefGoogle Scholar
  34. Whitfield M (1974) The hydrolysis of ammonium ions in sea water—a theoretical study. J Mar Biol Assoc UK 54:565–580CrossRefGoogle Scholar
  35. Zhang SY, Li G, Wu HB, Liu XG, Yao YH, Tao L, Liu H (2011) An integrated recirculating aquaculture system (RAS) for land-based fish farming: the effects on water quality and fish production. Aquac Eng 45:93–102CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.IfremerPalavas-les-FlotsFrance
  3. 3.UMR EcosymUSTLMontpellierFrance
  4. 4.University of MessinaMessinaItaly

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