Aquaculture International

, Volume 22, Issue 3, pp 1163–1174

Optimal conditions for cold-shock induction of triploidy in red tilapia

  • Padmaja Jayaprasad Pradeep
  • Thekkeparambil Chandrabose Srijaya
  • Anuar Hassan
  • Anil Kumar Chatterji
  • Boonsirm Withyachumnarnkul
  • Andrew Jeffs
Article

Abstract

Production of sterile triploid red tilapia [Oreochromis mossambicus (Mozambique tilapia); Peters, 1852 × Oreochromis niloticus (Nile tilapia); Linnaeus, 1758] is an effective strategy to overcome their prolific breeding. Optimal conditions for cold-shock induction of triploidy in red tilapia were investigated by experimentally examining two variables: appropriate temperature of the shock and duration of shock treatment. A constant time after insemination of 4 min was used to determine the best combination of temperature (6, 7, 8, 9, 11, 13, 15 °C) with different durations of shock (10, 20, 30, 40, 50 min) with resultant ploidy level verified karyotypically. Shock duration for 30 min at a temperature of 9 °C was found most effective in producing maximum triploidy (98.7 %) with higher rates of hatching (63.2 %) and survival up to yolk-sac stage (75.8 %). The chromosome count confirmed that triploid percentages were higher when cold shock was used for longer durations at each temperature; however, hatching rates were generally decreased. The maximum triploid yield (82.1 %) obtained was higher than the yield obtained using heat shock (72.7 %) in red tilapia previously. The application of the results of this study has the potential to greatly improve the production of triploid red tilapia in commercial aquaculture.

Keywords

Chromosome manipulation Sterile tilapia Sterile stock Red tilapia Triploidy induction 

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Padmaja Jayaprasad Pradeep
    • 1
    • 2
    • 3
  • Thekkeparambil Chandrabose Srijaya
    • 1
    • 4
  • Anuar Hassan
    • 1
  • Anil Kumar Chatterji
    • 1
  • Boonsirm Withyachumnarnkul
    • 2
    • 3
  • Andrew Jeffs
    • 5
  1. 1.Institute of Tropical AquacultureUniversity Malaysia TerengganuKuala TerengganuMalaysia
  2. 2.Department of Anatomy, Faculty of ScienceMahidol UniversityBangkokThailand
  3. 3.Faculty of Science, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp)Mahidol UniversityBangkokThailand
  4. 4.Department of Conservative Dentistry, Faculty of DentistryUniversity of MalayaKuala LumpurMalaysia
  5. 5.Leigh Marine LaboratoryUniversity of AucklandWarkworthNew Zealand

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