Aquaculture International

, Volume 22, Issue 3, pp 1163–1174

Optimal conditions for cold-shock induction of triploidy in red tilapia

  • Padmaja Jayaprasad Pradeep
  • Thekkeparambil Chandrabose Srijaya
  • Anuar Hassan
  • Anil Kumar Chatterji
  • Boonsirm Withyachumnarnkul
  • Andrew Jeffs


Production of sterile triploid red tilapia [Oreochromis mossambicus (Mozambique tilapia); Peters, 1852 × Oreochromis niloticus (Nile tilapia); Linnaeus, 1758] is an effective strategy to overcome their prolific breeding. Optimal conditions for cold-shock induction of triploidy in red tilapia were investigated by experimentally examining two variables: appropriate temperature of the shock and duration of shock treatment. A constant time after insemination of 4 min was used to determine the best combination of temperature (6, 7, 8, 9, 11, 13, 15 °C) with different durations of shock (10, 20, 30, 40, 50 min) with resultant ploidy level verified karyotypically. Shock duration for 30 min at a temperature of 9 °C was found most effective in producing maximum triploidy (98.7 %) with higher rates of hatching (63.2 %) and survival up to yolk-sac stage (75.8 %). The chromosome count confirmed that triploid percentages were higher when cold shock was used for longer durations at each temperature; however, hatching rates were generally decreased. The maximum triploid yield (82.1 %) obtained was higher than the yield obtained using heat shock (72.7 %) in red tilapia previously. The application of the results of this study has the potential to greatly improve the production of triploid red tilapia in commercial aquaculture.


Chromosome manipulation Sterile tilapia Sterile stock Red tilapia Triploidy induction 


  1. Alceste CS (2000) An overview of tilapia production systems. Aquacult Manage 26:47–51Google Scholar
  2. Al-Fageeh MB, Smales CM (2006) Control and regulation of the cellular responses to cold shock: the responses in yeast and mammalian systems. Biochem J Exp Zool 397:247–259Google Scholar
  3. Arai K, Wilkins NP (1987) Triploidization of brown trout (Salmo trutta) by heat shocks. Aquaculture 64:97–103CrossRefGoogle Scholar
  4. Baroiller JF (1996) Significant proportions of unexpected males in progenies from single pair mating with sibling sex reversed males of Oreochromis niloticus. In: Pullin RSV, Lazard J, Legendre M, Amon Kothias JB, Pauly D (eds) The third international symposium on tilapia in aquaculture. ICLARM conference proceedings 41, p 575Google Scholar
  5. Baroiller JF, Jalabert B (1989) Contribution of research in reproductive physiology to the culture of tilapias. Aquat Living Resour 2:105–116CrossRefGoogle Scholar
  6. Bramick U, Puckhaber B, Langholz HJ, Horstgen-Schwark G (1995) Testing of triploid Tilapia (Oreochromis niloticus) under tropical pond conditions. Aquaculture 137:343–353CrossRefGoogle Scholar
  7. Chourrout D, Itskovich J (1983) Three manipulations permitted by artificial insemination in tilapia: induced diploid gynogenesis, production of all triploid populations and intergeneric hybridization. In: Fishelson L, Yaron Z, (eds) Proceedings of the international symposium on tilapia in aquaculture, Tel Aviv University, Tel Aviv, Israel, 14, pp 246–255Google Scholar
  8. Colombo L, Barbaro A, Libertini A, Francescon A, Lombardo L (1995) Artificial fertilization and induction of triploidy and meiogynogenesis in the European sea bass Dicentrarchus labrax L. J Appl Ichthyol 11:118–125CrossRefGoogle Scholar
  9. Coward K, Bromage NR (2000) Reproductive physiology of female tilapia broodstock. Rev Fish Biol Fish 10:1–25CrossRefGoogle Scholar
  10. Dias da Silva FS, Moreira RG, Orozco-Zapata CR, Hilsdorf AWS (2007) Triploidy induction by cold shock in the South American catfish, Rhamdia quelen (Siluriformes) (Quoy & Gaimard, 1824). Aquaculture 272:110–114CrossRefGoogle Scholar
  11. Don J, Avtalion RR (1986) The induction of triploidy in Oreochromis aureus by heat shock. Theo Appl Genet 72:186–192CrossRefGoogle Scholar
  12. Don J, Avtalion RR (1988) Comparative study on the induction of triploidy in tilapias, using cold- and heat-shock techniques. J Fish Biol 32:665–672CrossRefGoogle Scholar
  13. El Gamal ARA, Davis KB, Jenkins JA, Torrans EL (1999) Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus (L.). J World Aquacult Soc 30(2):269–275Google Scholar
  14. Felip A, Zanuy S, Carrillo M, Martínez G, Ramos J, Piferrer F (1997) Optimal conditions for the induction of triploidy in the sea bass (Dicentrarchus labrax L.). Aquaculture 152:287–298CrossRefGoogle Scholar
  15. Hafeez-ur-Rehman M, Ahmed I, Khan N, Rasool F (2008) The culture performance of mono-sex and mixed-Sex tilapia in fertilized ponds. Int J Agr Biol 10:352–354Google Scholar
  16. Henken AM, Brunink AM, Richter CJJ (1987) Differences in growth rate and feed utilization between diploid and triploid African catfish, Clarias gariepinus Burchell 1822. Aquaculture 63:233–242CrossRefGoogle Scholar
  17. Hildebrandt B, Wust P, Ahlers O, Dieing A, Sreenivasa G, Kerner T, Felix R, Riess H (2002) The cellular and molecular basis of hyperthermia. Cri Rev Oncol Hem 43:33–56CrossRefGoogle Scholar
  18. Holmefjord I, Refstie T (1997) Induction of triploidy in Atlantic halibut by temperature shocks. Aquacult Int 5:169–173Google Scholar
  19. Hulata G (2001) Genetic manipulations in aquaculture: a review of stock improvement by classical and modern technologies. Genetica 111:155–173CrossRefPubMedGoogle Scholar
  20. Hussain MG (1996) Advancement in chromosome engineering research in fish: review of methods, achievements and applications. Asian Fish Sci 9:45–60Google Scholar
  21. Hussain MG (2004) Farming of tilapia: breeding plans, mass seed production and aquaculture techniques. Fisheries Research, Bangladesh, p 149Google Scholar
  22. Hussain MG, Chatterji A, McAndrew BJ, Johnstone R (1991) Triploidy induction in Nile tilapia, Oreochromis niloticus L. using pressure, heat and cold shocks. Theor Appl Genet 81:6–12CrossRefPubMedGoogle Scholar
  23. Karami A, Christianus A, Ishak Z, Courtenay SC, Syed MA, Noor Azlina M, Noorshinah H (2009) Effect of triploidization on juvenile African catfish (Clarias gariepinus). Aquacult Inter 18(5):851–858CrossRefGoogle Scholar
  24. Linhart O, Flajshans M, Kvasnika P (1991) Induced triploidy in the common carp (Cyprinus carpio L.): a comparison of two methods. Aquat Living Res 4:139–145CrossRefGoogle Scholar
  25. Lovshin LL, Da Silva AB, Carneiro-Sobrinho A, Melo FR (1990) Effects of Oreochromis niloticus females on the growth and yield of male hybrids (O. niloticus female x O. hornorum male) cultured in earthen ponds. Aquaculture 88:55–60CrossRefGoogle Scholar
  26. Mair GC (1993) Chromosome-set manipulation in tilapia- techniques, problems and prospects. Aquaculture 111:227–244CrossRefGoogle Scholar
  27. Maxime V (2008) The physiology of triploid fish: current knowledge and comparisons with diploid fish. Fish Fish 9:67–78CrossRefGoogle Scholar
  28. McGinty AS (1985) Effects of predation by largemouth bass in fish production ponds stocked with Tilapia nilotica. Aquaculture 46:269–274CrossRefGoogle Scholar
  29. Mol K, Byamungul N, Cuisset B, Yaron Z, Ofir M, Melard Ch, Castelli M, Kuhn ER (1994) Hormonal profile of growing male and female diploids and triploids of the blue tilapia, Oreochromis aureus, reared in intensive culture. Fish Physiol Biochem 13(3):209–218CrossRefPubMedGoogle Scholar
  30. Oliviero CA (2000) Regional review of aquaculture status and development trends in the Latin America and Caribbean. Book of synopses. NACA and FAO international conference on aquaculture in the third millennium, 20–25 February 2000, Bangkok, Thailand, pp 317–340Google Scholar
  31. Pandian TJ, Koteeswaran R (1998) Ploidy induction and sex control in fish. Hydrobiologia 384:167–243CrossRefGoogle Scholar
  32. Pandian TJ, Varadaraj K (1988) Techniques for producing all male and all triploid Oreochromis mossambicus. In: Pullin RSV, Bhukeswaran T, Tonguthai K, Maclean JL (eds) The second international symposium on tilapia in aquaculture, ICLARM conference proceedings 15. Department of Fisheries, Bangkok, Thailand and International Centre for Living Aquatic resources Management, Manila, Philippines, pp 243–249Google Scholar
  33. Penman DJ, Skibinski DOF, Beardmore JA (1987) Survival, growth rate and maturity in triploid tilapia. In: Tiews K (eds) Proceedings of the world symposium on selection, hybridization, and genetic engineering in aquaculture, vol II, 27–30 May 1986, Bordeaux, France. Heinemann, Berlin, pp. 277–287Google Scholar
  34. Piferrer F, Cal RM, Alvarez-Blazquez B, Sanchez L, Martinez P (2000) Induction of triploidy in the turbot (Scophthalmus maximus), ploidy determination and the effects of cold shocks. Aquaculture 188:79–90CrossRefGoogle Scholar
  35. Piferrer F, Cal RM, Gómez C, Bouza C, Martínez P (2003) Induction of triploidy in the turbot (Scophthalmus maximus), II. Effects of cold shock timing and induction of triploidy in a large volume of eggs. Aquaculture 220:821–831CrossRefGoogle Scholar
  36. Piferrer F, Beaumont A, Falguiere JC, Flajshans M, Haffray P, Colombo L (2009) Polyploid fish and shellfish: production, biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture 293:125–156CrossRefGoogle Scholar
  37. Pradeep PJ, Srijaya TC, Shahreeza MS, Mithun S, Anuar H, Anil A (2010) Induction of triploidy in red tilapia, Oreochromis mossambicus (Peters, 1852) × Oreochromis niloticus (Linnaeus, 1758) by heat shock treatment under laboratory conditions. J Coast Environ 1(1):91–102Google Scholar
  38. Pradeep PJ, Srijaya TC, Jose D, Papini A, Hassan A, Chatterji A (2011a) Identification of diploid and triploid Red Tilapia by using erythrocyte indices. Caryologia 64(4):485–492Google Scholar
  39. Pradeep PJ, Srijaya TC, Shaharom F, Chatterji A (2011b) Seed production and hatchery management techniques in tilapia. In: Shaharom F, Pradeep PJ, Anil CH (eds) Tilapia aquaculture techniques and potential. University of Malaysia Terengganu publication, p 340Google Scholar
  40. Pradeep PJ, Srijaya TC, Zain RB, Papini A, Chatterji A (2011c) A simple technique for chromosome preparation from embryonic tissues of teleosts for ploidy verification. Caryologia 64(2):235–241CrossRefGoogle Scholar
  41. Pradeep PJ, Srijaya TC, Aneesh M, Papini A (2012a) Can sterility through triploidy induction make any impact on tilapia industry? Internat J Aquat Sci 3(2):89–96Google Scholar
  42. Pradeep PJ, Srijaya TC, Aneesh M, Renjithkumar CR, Jose D, Papini A, Chatterji A (2012b) Triploidy induction in red tilapia. Caryologia 65(2):152–156CrossRefGoogle Scholar
  43. Pradeep PJ, Srijaya TC, Papini A, Anil CH (2012c) Effects of triploidy induction on growth and masculinization of red tilapia [Oreochromis mossambicus (Peters, 1852) × Oreochromis niloticus (Linnaeus, 1758)]. Aquaculture 344–349:181–187CrossRefGoogle Scholar
  44. Pradeep PJ, Srijaya TC, Anuar H, Chatterji A, Raghavan R, Withyachumnarnkul B, Jeffs A (2013) Growth performance of triploid red tilapia reared under laboratory conditions. J Appl Aquacult 25:176–189CrossRefGoogle Scholar
  45. Rothbard S, Pruginin Y (1975) Induced spawning and artificial incubation of tilapia. Aquaculture 5:315–321CrossRefGoogle Scholar
  46. Shelton WL, Phelps RP, Fitzpatrick M (1999) Production of monosex tilapia for aquaculture. Aqua News 14(1):1–3Google Scholar
  47. Tiwary BK, Kirubagaran R, Ray AK (2004) The biology of triploid fish. Rev Fish Biol Fish 14:391–402CrossRefGoogle Scholar
  48. Tsadik GG, Bart AN (2007) Effects of feeding, stocking density and water-flow rate on fecundity, spawning frequency and egg quality of Nile tilapia, Oreochromis niloticus (L.). Aquaculture 272:380–388CrossRefGoogle Scholar
  49. Uma B, Chandran MR (2008) Induction of triploidy in Gymnocorymbus ternetzi (Boulenger). Res J Fish Hydrobiol 3(2):41–47Google Scholar
  50. Valenti RJ (1975) Induced polyploidy in Tilapia aurea (Steindachner) by means of temperature shock treatment. J Fish Biol 7:519–528CrossRefGoogle Scholar
  51. Varadaraj K, Pandian TJ (1988) Induction of triploids in Oreochromis mossambicus by thermal, hydrostatic pressure and chemical shocks. In: Proceedings aquaculture international congress and exposition, pp 531–535Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Padmaja Jayaprasad Pradeep
    • 1
    • 2
    • 3
  • Thekkeparambil Chandrabose Srijaya
    • 1
    • 4
  • Anuar Hassan
    • 1
  • Anil Kumar Chatterji
    • 1
  • Boonsirm Withyachumnarnkul
    • 2
    • 3
  • Andrew Jeffs
    • 5
  1. 1.Institute of Tropical AquacultureUniversity Malaysia TerengganuKuala TerengganuMalaysia
  2. 2.Department of Anatomy, Faculty of ScienceMahidol UniversityBangkokThailand
  3. 3.Faculty of Science, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp)Mahidol UniversityBangkokThailand
  4. 4.Department of Conservative Dentistry, Faculty of DentistryUniversity of MalayaKuala LumpurMalaysia
  5. 5.Leigh Marine LaboratoryUniversity of AucklandWarkworthNew Zealand

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