Aquaculture International

, Volume 22, Issue 2, pp 607–617 | Cite as

Dietary supplementation with Bacillus can improve the growth and survival of the kuruma shrimp Marsupenaeus japonicus in high-temperature environments

  • Hong-biao Dong
  • Yong-quan Su
  • Yong Mao
  • Xin-xin You
  • Shao-xiong Ding
  • Jun Wang
Article
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Accepted:

Abstract

This study examined the effects of a commercial Bacillus complex used as probiotics on the performance of Marsupenaeus japonicus under high water temperature after 60 days of rearing. Growth and survival rate of M. japonicus were significantly increased in the probiotics-treated group (P < 0.05). Vibrio count and percentage of Vibrio in the intestinal tracts of M. japonicus reared with Bacillus were significantly lower than in other treatments (P < 0.05). The expressions of immune-related gene including prophenoloxidase, lysozyme, cytosolic manganese superoxide dismutase and hemocyanin subunit L in M. japonicus were significantly increased in the Bacillus-treated group (P < 0.05). The results indicated that Bacillus can be used as a diet supplement to minimize the damages caused by free radicals generated from insufficient oxygen metabolism due to the high-temperature stress, and to enhance immunity and activate the immune response levels. Our findings can improve the growth and survival rates of M. japonicus during the high-temperature farming period.

Keywords

Bacillus Marsupenaeus japonicus Growth Intestinal bacteria Immune status High temperature 
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Notes

Acknowledgments

This study was supported by the State 863 Project of China (Grant No. 2012AA10A409-03) and the Project of China Agriculture Research System (Grant No. CARS-47). Dongshan Maoxin Aquaculture Company (Dongshan County, Fujian Province) provided the experimental sites and equipment.

References

  1. Adachi K, Hirata T, Nishioka T, Sakaguchi M (2003) Hemocyte components in crustaceans convert hemocyanin into a phenoloxidase-like enzyme. Comp Biochem Physiol B 134:135–141PubMedCrossRefGoogle Scholar
  2. Atlas RM, Horowitz A, Krichevsky M, Bej AK (1991) Response of microbial populations to environmental disturbance. Microbiol Ecol 22:249–256CrossRefGoogle Scholar
  3. Burge EJ, Madigan DJ, Burnett LE, Burnett KG (2007) Lysozyme gene expression by hemocytes of Pacific white shrimp, Litopenaeus vannamei, after injection with Vibrio. Fish Shellfish Immunol 22:327–339PubMedCrossRefGoogle Scholar
  4. Campa-Córdova AI, Hernández-Saaverdra NY, Ascencio F (2002) Superoxide dismutase as modulator of immune function in American white shrimp (Litopenaeus vannamei). Comp Biochem Physiol C 133:557–565Google Scholar
  5. Cerenius L, Lee BL, Söderhäll K (2008) The proPO-system: pros and cons for its role in invertebrate immunity. Trends Immunol 29:263–271PubMedCrossRefGoogle Scholar
  6. Cheng WT, Wang LU, Chen JC (2005) Effect of water temperature on the immune response of white shrimp Litopenaeus vannamei to Vibro alginolyticus. Aquaculture 250:592–601CrossRefGoogle Scholar
  7. Cui YD, Du YZ, Lu MX, Qiang CK (2011) Antioxidant responses of Chilo suppressalis (Lepidoptera: Pyralidae) larvae exposed to thermal stress. J Therm Biol 36:292–297CrossRefGoogle Scholar
  8. Decamp O, Moriarty DJW, Lavens P (2008) Probiotics for shrimp larviculture: review of field data from Asia and Latin America. Aquac Res 39:334–338CrossRefGoogle Scholar
  9. Destoumieux-Garzon D, Saulnier D, Gamier J, Jouffrey C, Bulet P, Bachere E (2001) Crustacean Immunity: antifungal peptides are generated from the C terminus of shrimp hemocyanin in response to microbial challenge. J Biol Chem 276:47070–47077PubMedCrossRefGoogle Scholar
  10. Farzanfar A (2006) The use of probiotics in shrimp aquaculture. FEMS Immunol Med Microbiol 48:149–158PubMedCrossRefGoogle Scholar
  11. Fridovich I (1995) Superoxide radical and superoxide dismutases. Annu Rev Biochem 64:97–112PubMedCrossRefGoogle Scholar
  12. Greg JC, Peter JC, Nigel PP, Don F (2002) The effects of temperature on the growth, survival and biomass of different families of juvenile Penaeus japonicus Bate. Aquaculture 214:185–199CrossRefGoogle Scholar
  13. Hewitt DR, Duncan PF (2001) Effect of high water temperature on the survival, moulting and food consumption of Penaeus (Marsupenaeus) japonicus (Bate, 1888). Aquac Res 32:305–313CrossRefGoogle Scholar
  14. Jollès P, Jollès J (1984) What’s new in lysozyme research? Always a model system, today as yesterday. Mol Cell Biochem 63:165–189PubMedCrossRefGoogle Scholar
  15. José LB, Tyrone RL (2007) Inhibitory activity of probiotic Bacillus subtilis UTM126 against Vibrio species confers protection against Vibriosis in juvenile shrimp (Litopenaeus vannamei). Curr Microbiol 55:409–412CrossRefGoogle Scholar
  16. José LB, Blas I, Ruiz-Zarzuela I, Cunningham D, Vendrella D, Múzquiz JL (2006) The role of probiotics in aquaculture. Vet Microbiol 114:173–186CrossRefGoogle Scholar
  17. Karunasagar I, Pai R, Malathi GR, Karunasagar I (1994) Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio harveyi infection. Aquaculture 128:203–209CrossRefGoogle Scholar
  18. Keysami MA, Saad CR, Sijam K, Daud HM, Alimon AR (2007) Effect of Bacillus subtilis on growth development and survival of post larvae Macrobrachium rosenbergii (de Man). Aquac Nutr 13:131–136CrossRefGoogle Scholar
  19. Lavilla-Pitogo CR, Leano EM, Paner MG (1998) Mortalities of pond-cultured juvenile shrimp, Penaeus monodon, associated with dominance of luminescent Vibriosis in the rearing environment. Aquaculture 164:337–349CrossRefGoogle Scholar
  20. Liao IC, Chen YH (1994) Culture of Kuruma prawn (Penaeus japonicus Bate) in Asia. Special Rep World Aquac 25: 18–33Google Scholar
  21. Liu CH, Chiu CS, Ho PL, Wang SW (2009) Improvement in the growth performance of white shrimp, Litopenaeus vannamei, by a protease producing probiotic, Bacillus subtilis E20 from natto. J Appl Microbiol 107:1031–1041PubMedCrossRefGoogle Scholar
  22. Liu KF, Chiu CH, Shiu YL, Cheng WT, Liu CH (2010) Effects of the probiotic, Bacillus subtilis E20, on the survival, development, stress tolerance, and immune status of white shrimp, Litopenaeus vannamei larvae. Fish Shellfish Immunol 28:837–844PubMedCrossRefGoogle Scholar
  23. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and 2−ΔΔCT method. Methods 25:402–408PubMedCrossRefGoogle Scholar
  24. Lopez-Martinez G, Elnitsky MA, Benoit JB, Lee RE, Denlinger DL (2008) High resistance to oxidative damage in the Antarctic midge Belgica antarctica, and developmentally linked expression of genes encoding superoxide dismutase, catalase and heat shock proteins. Insect Biochem Mol Biol 38:796–804PubMedCrossRefGoogle Scholar
  25. Mariel G, Fabiano T, Jenny R (2004) Selection of probiotic bacteria and study of their immunostimulatory effect in Penaeus vannamei. Aquaculture 233:1–14CrossRefGoogle Scholar
  26. Moriarty DJW (1997) The role of microorganisms in aquaculture ponds. Aquaculture 151:333–349CrossRefGoogle Scholar
  27. Moriarty DJW (1998) Control of luminous Vibrio species in penaeid aquaculture ponds. Aquaculture 164:351–358CrossRefGoogle Scholar
  28. Nakayama T, Lu H, Nomura N (2009) Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp. Lett Appl Microbiol 49:679–684PubMedCrossRefGoogle Scholar
  29. Ochoa-Solano JL, Olmos-Soto J (2006) The functional property of Bacillus for shrimp feeds. Food Microbiol 23:519–525CrossRefGoogle Scholar
  30. Rengpipat S, Rukpratanporn S, Piyatiratitivorakul S, Menasaveta P (1998) Effect of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture 167:301–313CrossRefGoogle Scholar
  31. Rengpipat S, Rukpratanporn S, Piyatiratitivorakul S, Menasaveta P (2000) Immunity enhancement in black tiger shrimp (Penaeus monodon) by a probiont bacterium (Bacillus S11). Aquaculture 191:271–288Google Scholar
  32. Shen WY, Fu LL, Li WF, Zhu YR (2010) Effect of dietary supplementation with Bacillus subtilis on the growth, performance, immune response and antioxidant activities of the shrimp (Litopenaeus vannamei). Aquac Res 41:1691–1698CrossRefGoogle Scholar
  33. Söderhäll K, Cerenius L (1998) Role of the prophenoloxidase-activating system in invertebrate immunity. Curr Opin Immunol 10:23–28PubMedCrossRefGoogle Scholar
  34. Tseng DY, Ho PL, Huang SY, Cheng SC, Shiu YL, Chiu CS, Liu CH (2009) Enhancement of immunity and disease resistance in the white shrimp, Litopenaeus vannamei, by the probiotic, Bacillus subtilis E20. Fish Shellfish Immunol 26:339–344PubMedCrossRefGoogle Scholar
  35. Vaseeharan B, Ramasamy P (2003) Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Lett Appl Microbiol 36:83–87PubMedCrossRefGoogle Scholar
  36. Verschuere L, Rombaut G, Sorgeloos P, Verstraete W (2000) Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64:655–671PubMedCentralPubMedCrossRefGoogle Scholar
  37. Wang YB (2007) Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei. Aquaculture 269:259–264CrossRefGoogle Scholar
  38. Wang W, Zhang XB (2008) Comparison of antiviral efficiency of immune responses in shrimp. Fish Shellfish Immunol 25:522–527PubMedCrossRefGoogle Scholar
  39. You XX, Su YQ, Mao Y, Liu M, Wang J, Zhang M, Wu C (2010) Effect of high water temperature on mortality, immune response and viral replication of WSSV-infected Marsupenaeus japonicus juveniles and adults. Aquaculture 305:133–137CrossRefGoogle Scholar
  40. Zhou XX, Wang YB, Li WF (2009) Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality, survival rate and digestive enzyme activities. Aquaculture 287:349–353CrossRefGoogle Scholar
  41. Zhou J, Wang L, Xin Y, Wang WN, He WY, Wang AL, Liu Y (2010) Effect of temperature on antioxidant enzyme gene expression and stress protein response in white shrimp, Litopenaeus vannamei. J Therm Biol 35:284–289CrossRefGoogle Scholar
  42. Ziaei-Nejad S, Rezaei MH, Takami GA, Lovett DL, Mirvaghefi AR, Shakouri M (2006) The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture 252:516–524CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Hong-biao Dong
    • 1
  • Yong-quan Su
    • 1
  • Yong Mao
    • 1
  • Xin-xin You
    • 1
  • Shao-xiong Ding
    • 1
  • Jun Wang
    • 1
  1. 1.State Key Laboratory of Marine Environmental Science, College of Ocean and Earth SciencesXiamen UniversityXiamenChina

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