Effects of phytase pretreatment of soybean meal and phytase-sprayed in diets on growth, apparent digestibility coefficient and nutrient excretion of rainbow trout (Oncorhynchus mykiss Walbaum)

  • Feng Wang
  • Yu-Hong Yang
  • Zhi-Zhong Han
  • Hong-Wei Dong
  • Cheng-Hui Yang
  • Zuo-Yu Zou


Rainbow trout were fed a diet containing phytase-sprayed and phytase-pretreated soybean meal with different phytase levels. The single factor random block design was used to analyze the effects on rainbow trout of dietary phytase supplementation on growth performance, nutritional ingredient digestibility and nutrient excretion. After 90 days, the results showed that feed conversion ratio (FCR) and protein efficiency ratio (PER) were significantly improved and specific growth rate (SGR) was not affected by spraying phytase, but SGR, FCR and PER were not significantly improved by phytase pretreatment. A digestibility trial conducted after the feeding trial showed that apparent digestibility coefficient (ADC) of diet protein and minerals was increased with phytase supplementation. However, there was a negative effect of phytase on the ADC of lipid. The excretion experiment showed that the supplementation of phytase resulted in decreased nutrient excretion in feces, but lipid excretion was slightly increased with phytase supplementation. In addition, the results of P excretion and ADC of P analyzed by t-test showed that phytase pre-treatment method should be a more rational method than the spraying method. The results of SGR, ADC of P and P excretion analyzed by quadratic regression indicated that 2,000–3,000 U/kg levels by the spraying method could be a rational range of phytase supplementation, and about 1,000 U/kg should be an optimal level by the pretreatment method. Thus, use of phytase in rainbow trout feeds can have economic and environmental benefits.


Phytase Rainbow trout Growth Apparent digestibility coefficient Nutrient excretion 



Analysis of variance


Apparent digestibility coefficient


Specific growth rate


Feed conversion ratio


Protein efficiency ratio



The place of study was supported by Harbin Academy of Agricultural Sciences Fisheries Research Institute and the experiment was financially supported by grant # 2005AA6CN183 from Harbin Science and Technology Project.


  1. AOAC (1990) Official methods of analysis of Official Analytical Chemists International, 15th edn. Association of Official Analytical Chemists, ArlingtonGoogle Scholar
  2. AOAC (1995) Official methods of analysis of Official Analytical Chemists International, 16th edn. Association of Official Analytical Chemists, ArlingtonGoogle Scholar
  3. Bergheim A, Aabel JP, Seymour EA (1991) Past and present approaches to aquaculture waste management in Norwegian net pen culture operations. In: Cowey CB, Cho CY (eds) Nutritional strategies and aquaculture waste. Proceedings of the first international symposium on nutritional strategies in management of aquaculture waste, 2–6 June 1990, Guelph, Canada. University of Guelph, Ontario, Canada, pp 117–136Google Scholar
  4. Brown PB (1993) Comparison of fecal collection methods for determining P absorption in rainbow trout. In: Kaushik SJ, Luquet P (eds) Fish nutrition in practice, Biarritz (France), June 24–27, 1991. Ed. INRA, Paris 1993 (Les Colloques, no. 61), pp 443–447Google Scholar
  5. Cain KD, Garling DL (1995) Pretreatment of soybean meal with phytase for salmonid diets to reduce P concentrations in hatchery effluents. Prog Fish-Cult 57:114–119CrossRefGoogle Scholar
  6. Cheng ZJ, Hardy RW (2002) Effect of microbial phytase on apparent nutrient digestibility of barley, canola meal, wheat and wheat middlings, measured in vivo using rainbow trout (Oncorhynchus mykiss). Aquac Nutr 8:271–277CrossRefGoogle Scholar
  7. Cheng ZJ, Hardy RW (2003) Effects of extrusion and expelling processing, and microbial phytase supplementation on apparent digestibility coefficients of nutrients in full-fat soybeans for rainbow trout (Oncorhynchus mykiss). Aquaculture 218:501–514CrossRefGoogle Scholar
  8. Cheryan M (1980) Phytic acid interactions in food systems. Crit Rev Food Sci Nutr 13:297–335PubMedCrossRefGoogle Scholar
  9. Fontainhas-Femandes A, Gomes E, Reis-Henriques MA, Coimbra J (1999) Replacement of fish meal by plant protein in the diet of Nile tilapia: digestibility and growth performance. Aquac Int 7:57–67CrossRefGoogle Scholar
  10. Forster I, Higgs DA, Dosanjh BS, Rowshandeli M, Parr J (1999) Potential for dietary phytase to improve the nutritive value of canola protein concentrate and decrease P output in rainbow trout (Oncorhynchus mykiss) held in 11°C fresh water. Aquaculture 179:109–125CrossRefGoogle Scholar
  11. Francis G, Makkar HPS, Becker K (2001) Antinutritional factors present in plant-derived alternate ash feed ingredients and their effects in ash. Aquaculture 99:197–227CrossRefGoogle Scholar
  12. Helland SJ, Grisdale-Helland B, Nerland S (1996) A simple method for the measurement of daily feed intake of groups of fish in tanks. Aquaculture 139:157–163CrossRefGoogle Scholar
  13. Jackson LS, Li MH, Robinson EH (1996) Use of microbial phytase in channel catfish (Ictalurus punctatus) diets to improve utilization of phytate P. J World Aquac Soc 27:309–313CrossRefGoogle Scholar
  14. Jahan P, Watanabe T, Kiron V, Satoh S (2003) Improved carp diets based on plant protein sources reduce environmental P loading. Fish Sci 69:219–225CrossRefGoogle Scholar
  15. Lall SP (1991) Digestibility, metabolism and excretion of dietary P in fish. In: Cowey CB, Cho CY (eds) Nutritional strategies and aquaculture waste. Proceedings of the first international symposium on nutritional strategies in management of aquaculture waste. University of Guelph, Ontario, Canada, pp 21–36Google Scholar
  16. Lall SP (2002) The minerals. In: Halver JE, Hardy RW (eds) Fish nutrition. Academic Press, San Diego, pp 259–308Google Scholar
  17. Lanari D, D’Agaro E, Turri C (1998) Use of nonlinear regression to evaluate the effects of phytase enzyme treatment of plant protein diets for rainbow trout (Oncorhynchus mykiss). Aquaculture 161:345–356CrossRefGoogle Scholar
  18. Liu BL, Rafing A, Tzeng YM, Rob A (1998) The induction and characterization of phytase and beyond. Enzyme Microb Technol 22:415–424CrossRefGoogle Scholar
  19. Mabahinzireki GB, Dabrowski K, Lee KJ, EI-Saidy D, Wisner ER (2001) Growth, fed utilization and body composition of tilapia (Oreochromis sp.) fed with cottonseed meal-based diets in a recirculating system. Aquac Nutr 7:189–200CrossRefGoogle Scholar
  20. Masumoto T, Tamura B, Shimeno S (2001) Effects of phytase on bioavailability of P in soybean meal-based diets for Japanese flounder (Paralichthys olivaceus). Fish Sci 67:1075–1080CrossRefGoogle Scholar
  21. National Research Council (1993) Nutrient requirements of fish. National Academic Press, Washington, DCGoogle Scholar
  22. Oliva-Teles A, Pereira JP, Gouveia A, Gomes E (1998) Utilization of diets supplemented with microbial phytase by seabass (Dicentrarchus labrax) juveniles. Aquat Living Resour 11:255–259CrossRefGoogle Scholar
  23. Papatryphon E, Howell RA, Soares JH Jr (1999) Growth and mineral absorption by striped bass (Morone saxatilis) fed a plant feedstuff based diet supplemented with phytase. J World Aquac Soc 30:161–173CrossRefGoogle Scholar
  24. Papatryphon E, Soares JH Jr (2001) The effect of phytase on apparent digestibility of four practical plant feedstuffs fed to striped bass (Morone saxatilis). Aquac Nutr 7:161–167CrossRefGoogle Scholar
  25. Powers Hughes K, Soares JH Jr (1998) Efficacy of phytase on P utilization in practical diets fed to striped bass (Morone saxatilis). Aquac Nutr 4:133–140CrossRefGoogle Scholar
  26. Riche M, Brown PB (1996) Availability of P from feedstuffs fed to rainbow trout (Oncorhynchus mykiss). Aquaculture 142:269–282CrossRefGoogle Scholar
  27. Rodehutscord M (1996) Response of rainbow trout (Oncorhynchus mykiss) growing from 50 to 200 g to supplements of dibasic sodium phosphate in a purified diet. J Nutr 126:324–331PubMedGoogle Scholar
  28. Rodehutscord M, Pfeffer E (1995) Effects of supplemental microbial phytase on P digestibility and utilization in rainbow trout (Oncorhynchus mykiss). Water Sci Technol 31:143–147CrossRefGoogle Scholar
  29. Sajjadi M, Carter CG (2004) Dietary phytase supplementation and the utilization of P by Atlantic salmon (Salmo salar L.) fed a canola-meal-based diet. Aquaculture 240:417–431CrossRefGoogle Scholar
  30. Schaefer A, Koppe WM, Meyer-Burgdorff KH, Gunther KD (1995) Effects of microbial phytase on utilization of native P by carp in a diet based on soybean meal. Water Sci Technol 31:149–155CrossRefGoogle Scholar
  31. Singh M, Krikorian AD (1982) Inhibition of trypsin activity in vitro by phytate. J Agric Food Chem 30:799–800CrossRefGoogle Scholar
  32. Slominski BA, Davie T, Nyachoti MC, Jones O (2007) Heat stability of endogenous and microbial phytase during feed pelleting. Livest Sci 109:244–246CrossRefGoogle Scholar
  33. Snedecor GW, Concbran WG (1978) Statistical methods, 6th edn. Iowa State University Press, AmesGoogle Scholar
  34. Spinelli J, Houle CR, Wekell JC (1983) The effect of phytates on the growth of rainbow trout (Salmo gairdneri) fed purified diets containing varying quantities of Ca and Mg. Aquaculture 30:71–83CrossRefGoogle Scholar
  35. Storebakken T, Shearer KD, Roem AJ (1998) Availability of protein, P and other elements in fish meal, soy-protein concentrate and phytase-treated soy-protein-concentrate-based diets to Atlantic salmon (Salmo salar). Aquaculture 161:365–379CrossRefGoogle Scholar
  36. Sugiura SH, Dong FM, Hardy RW (1998) Effects of dietary supplements on the availability of minerals in fish meal; preliminary observations. Aquaculture 160:283–303CrossRefGoogle Scholar
  37. Sugiura SH, Gabaudan J, Dong FM, Hardy RW (2001) Dietary microbial phytase supplementation and the utilization of P, trace minerals and protein by rainbow trout [Oncorhynchus mykiss (Walbaum)] fed soybean meal-based diets. Aquac Res 32:583–592CrossRefGoogle Scholar
  38. Teskeredzic Z, Higgs DA, Dosanjh BS, McBride JR, Hardy RW, Beames RM, Simell M, Vaara T, Bridges RB (1995) Assessment of unphytinized and dephytinized rapeseed protein concentrate as sources of dietary protein for juvenile rainbow trout (Oncorhynchus mykiss). Aquaculture 131:261–277CrossRefGoogle Scholar
  39. Vielma J, Lall SP, Koskela J, Schoner FJ, Mattila P (1998) Effects of dietary phytase and cholecalciferol on P bioavailability in rainbow trout (Oncorhynchus mykiss). Aquaculture 163:309–323CrossRefGoogle Scholar
  40. Vielma J, Mäkinen T, Ekholm P, Koskela J (2000) Influence of dietary soy and phytase levels on performance and body composition of large rainbow trout (Oncorhynchus mykiss) and algal availability of P load. Aquaculture 183:349–362CrossRefGoogle Scholar
  41. Vielma J, Ruohonen K, Peisker M (2002) Dephytinization of two soy proteins increases P and protein utilization by rainbow trout (Oncorhynchus mykiss). Aquaculture 204:145–156CrossRefGoogle Scholar
  42. Vielma J, Ruohonen K, Gabaudan J, Vogel K (2004) Top-spraying soybean meal-based diets with phytase improves protein and mineral digestibilities but not lysine utilization in rainbow trout [Oncorhynchus mykiss (Walbaum)]. Aquac Res 35:955–964CrossRefGoogle Scholar
  43. Wise A (1983) Dietary factors determining the biological activities of phytate. Nutr Abs Rev 53:791–807Google Scholar
  44. Yan W, Reigh R, Xu Z (2002) Effects of fungal phytase on utilization of dietary protein and minerals, and dephosphorylation of phytic acid in the alimentary tract of channel catfish (Ictalurus punctatus) fed an all-plant-protein diet. J World Aquac Soc 33:10–22CrossRefGoogle Scholar
  45. Yoo GY, Wang X, Choi S, Han K, Kang JC, Bai SC (2005) Dietary microbial phytase increased the P digestibility in juvenile Korean rockfish (Sebastes schlegeli) fed diets containing soybean meal. Aquaculture 243:315–322CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Feng Wang
    • 1
  • Yu-Hong Yang
    • 1
  • Zhi-Zhong Han
    • 2
  • Hong-Wei Dong
    • 2
  • Cheng-Hui Yang
    • 2
  • Zuo-Yu Zou
    • 2
  1. 1.College of Animal Science and TechnologyNortheast Agricultural UniversityHarbinPeople’s Republic of China
  2. 2.Fisheries Research InstituteHarbin Academy of Agricultural SciencesHarbinPeople’s Republic of China

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