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Aquaculture International

, Volume 16, Issue 6, pp 497–510 | Cite as

Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio

  • Wei Guo
  • Jian-Fang GuiEmail author
Article

Abstract

Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp.

Keywords

Gibel carp Microsatellite Gynogenesis Polyploidy Molecular marker 

Notes

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (30630050), the National Major Basic Research Program (2004CB117406), and the National Tackle Key Problem Program (Grant No. 2006BAD01A1206).

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiologyChinese Academy of SciencesWuhanChina
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingChina
  3. 3.Institution of Biological and Medical TechnologyFuzhou UniversityFuzhouChina

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