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Aquaculture International

, Volume 15, Issue 1, pp 1–9 | Cite as

Fatty acid composition of 12 microalgae for possible use in aquaculture feed

  • Vishwanath Patil
  • Torsten Källqvist
  • Elisabeth Olsen
  • Gjermund Vogt
  • Hans R. Gislerød
Original Paper

Abstract

Twelve algal strains representing the classes Cyanophyceae, Prymnesiophyceae, Bacillariophyceae, Rhodophyceae, Cryptophyceae, Chlorophyceae, Xantophyceae and Eustigmatophyceae were selected mainly from the culture collection of the Norwegian Institute for Water Research (NIVA). The algae were grown as continuous cultures in a 1.8 l. reactor, internally illuminated with an 11 W fluorescent tube. The retention time was adjusted in the range 2–4 days to fit the growth rate of the algae. The growth responses and fatty acid composition were analysed. The maximum production rate was obtained with Pseudokirchneriella subcapitata (0.63 g 1−1 day−1) and the lowest with Porphyridium cruentum 0.13 g 1−1 day−1. Arachidonic acid (AA) and eicosapentaenoic acid (EPA) were the dominating polyunsaturated fatty acids (PUFAs) in P. cruentum, while only EPA accumulated in Phaeodactylum tricornutum. Docosahexaenoic acid (DHA) was the major PUFA in Isochrysis galbana, while Pavlova sp. had both EPA and DHA. This is the first report on the fatty acid profiles of Nannochloropsis oceanica, Chroococcus sp., Synechococcus sp. and Tribonema sp.

Keywords

Continuous culture Growth media Photobioreactor Polyunsaturated fatty acids Productivity 

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Notes

Acknowledgements

This project was financed through research grant 146860/120 from the Research Council of Norway. We thank Karin Svinset and Anne Skivik Jørgensen for technical assistance. We also thank the referees for their valuable comments.

References

  1. Ackman RG, Tocher CS, Mc Lachland J (1968) Marine phytoplanter fatty acids. J Fisheries Res Board Canada 25:1603–1620Google Scholar
  2. Apt KE, Behrens PW (1999) Commercial developments in microalgal biotechnology. J Phycol 35:215–226CrossRefGoogle Scholar
  3. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917PubMedGoogle Scholar
  4. Cohen Z, Vonshak A, Richmond A (1988) Effect of environmental conditions on fatty acid composition of the red alga Porphyridium cruentum: correlation to growth rate. J Phycol 24:328–332Google Scholar
  5. Cranwell PA, Jaworski GHM, Bidey HM (1990) Hydrocarbons, sterols, esters and fatty acids in six freshwater chlorophytes. Phytochemistry 29:145–151CrossRefGoogle Scholar
  6. Crawford M (2000) Placental delivery of arachidonic and docosahexaenoic acids: implication of preterm infants. Am J Clin Nutr 71:275S–284SPubMedGoogle Scholar
  7. Drevon CA, Baksaas I, Krokan HE (eds) (1993) Omega-3 fatty acids: metabolism and biological effects. Birkhauser, Basel, Switzerland, pp 389Google Scholar
  8. FAO (2006) Fishery Information, Data and Statistics Unit (FIDI). c2002. Fishery Statistical Collections. FIGIS Data Collection. FAO, Rome. http://www. fao. org:80//figis/servlet/static? dom =collection&xml=global-aquaculture-production.xml
  9. Horrocks LA, Yeo YK (1999) Health benefits of docosahexaenoic acid. Pharmacol Res 40:211–225PubMedCrossRefGoogle Scholar
  10. Kanazawa A, Teshima S, Sakamoto M (1985) Effects of dietary lipids, fatty acids and phospholipids on growth and survival of prawn (Penaues japonicus) larvae. Aquaculture 130:159–167Google Scholar
  11. Kates M, Volcani BE (1966) Lipid components of diatoms. Biochem Biophys Acta 116:264–278Google Scholar
  12. Kitano M, Matsukawa R, Karube I (1997) Changes in eicosapentaenoic acid content of Navicula saprophila, Rhodomonas salina and Nitzchia sp. under mixotropic conditions. J Appl Phycol 9:559–563Google Scholar
  13. Knauer J, Southgate PC (1999) A review of the nutritional requirements of bivalves and the␣development of alternative and artificial diets for bivalve aquaculture. Rev Fisheries Sci 7:241–280Google Scholar
  14. Kotai J (1972) Instructions for preparation of modified nutrient solution Z8 for algae. Publication B-11/(69) Norsk institutt for vannforskning, Oslo, 5 ppGoogle Scholar
  15. Lavens P, Sorgeloos P, Dhert P, Devresse B (1995) Larval foods. In: Bromage N, Roberts R (eds) Broodstock management and egg and larval quality, vol 99. Blackwell Scientific, Oxford, pp 373–397Google Scholar
  16. Maruyama I, Nakamura T, Matsubayashi T, Ando Y, Maeda T (1986) Identification of the algae known as ‘marine Chlorella’ as a member of the Eustigmatophyceae. J Phycol 34:319–325Google Scholar
  17. Mason ME, Waller GR (1964) Dimethoxypropane induces trans-esterification of fats and oils in press of methylesters for gas chromatographic analysis. Anal Chem 36:583CrossRefGoogle Scholar
  18. McLamon-Riches CJ, Rolph CE, Greenway DLA, Robinson PK (1998) Effects of environmental factors and metals on Selenastrum capricornutum lipids. Phytochemistry 49:1241–1247CrossRefGoogle Scholar
  19. Naylor RL, Burke M (2005) Aquaculture and ocean resources: raising tigers of the sea. Ann Rev Environ Resourc 30:185–218CrossRefGoogle Scholar
  20. Nettleton AJ (eds) (1995) Omega-3 fatty acids and health. Chapman and Hall, New York, pp 359Google Scholar
  21. Patil V, Gislerød HR (2006) The importance of omega-3 fatty acids in diet. Curr Sci 90:908–909Google Scholar
  22. Patil V, Reitan KI, Knutsen G, Mortensen LM, Källqvist T, Olsen E, Vogt G, Gislerød HR (2005) Microalgae as source of polyunsaturated fatty acids for aquaculture. Curr Topics Plant Biol 6:57–65Google Scholar
  23. Reitan KI, Rainuzzo JR, Olsen Y (1994) Effect of nutrient limitation on fatty acid and lipid content of marine microalgae. J Phycol 30:972–979CrossRefGoogle Scholar
  24. Reitan KI, Rainuzzo JR, Oie G, Olsen Y (1997) A review of the nutritional effects of algae in marine fish larvae. Aquaculture 155:207–221CrossRefGoogle Scholar
  25. Renaud SM, Parry DL, Tinh L (1994) Microalgae for use in tropical aquaculture: I. Gross chemical and fatty acid compositions of twelve species of microalgae from Northern Territory, Australia. J␣Appl Phycol 6:337–345CrossRefGoogle Scholar
  26. Robelloso Fuentes MM, Acién Fernández FG, Sánchez Pérez JA, Guil Guerrero JL (2000) Biomass nutrient profiles of microalga Poryphyridium cruentum. Food Chem 70:345–353CrossRefGoogle Scholar
  27. Shahidi F, Wanasundara UN (1998) Omega-3 fatty acid concentrates: nutritional aspects and production technologies. Trends Food Sci Technol 9:230–240CrossRefGoogle Scholar
  28. Simopoulos AP (1999) Essential fatty acids in health and chronic disease. Am J Clin Nutr 70:560–569Google Scholar
  29. Staub R (1961) Ernärungsphysiologische Untersuchungen an der planktischen Blaualge Oscillatoria rubescens D.C. Schweiz Z Hydrol 23:82–198CrossRefGoogle Scholar
  30. Suda S, Atsumi M, Miyashita H (2002) Taxonomic characterization of marine Nannochloropsis species, N. oceanica sp. nov. (Eustigmatophyceae). Phycologia 41:273–279CrossRefGoogle Scholar
  31. Sukenik A (1991) Ecophysiological considerations in optimization of eicosapentaenoic production by Nannochloropsis sp. (Eustigmatophyceae). Bioresourc Technol 35:263–370CrossRefGoogle Scholar
  32. Volkman JK, Jeffrey SW, Nichols PD, Rodgers GI, Garland CD (1989) Fatty acid and lipid composition of 10 species of microalgae used in mariculture. J Exp Mar Biol Ecol 128:219–240CrossRefGoogle Scholar
  33. Watanabe T (1983) The importance of docosahexaenoic acid in marine larval fish. J World Aquacult Soc 24:152–161Google Scholar
  34. Yongmanitchai W, Ward OP (1989) Omega-3 fatty acid: alternative sources of production. Process Biochem 24:117–125Google Scholar
  35. Yongmanitchai W, Ward OP (1991) Growth and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions. Appl Env Microbiol 57:419–425Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Vishwanath Patil
    • 1
  • Torsten Källqvist
    • 2
  • Elisabeth Olsen
    • 3
    • 4
  • Gjermund Vogt
    • 3
  • Hans R. Gislerød
    • 1
  1. 1.Department of Plant and Environmental SciencesNorwegian University of Life SciencesBlindernNorway
  2. 2.Norwegian Institute for Water Research (NIVA)KjelsaasNorway
  3. 3.The Norwegian Food Research InstituteOsloveienNorway
  4. 4.Department of Chemistry, Biotechnology and Food Sciences, Norwegian University of Life Sciences, P. O. Box 5003N-1432 ÅsNorway

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