Concentration, Molecular Weight Distribution and Neutral Sugar Composition of DOC in Maritime Antarctic Lakes of Differing Trophic Status Article Received: 01 April 2005 Accepted: 06 October 2005 DOI:
Cite this article as: Quayle, W.C. & Convey, P. Aquat Geochem (2006) 12: 161. doi:10.1007/s10498-005-3882-x Abstract
The molecular weight distributions and hydrolysable neutral sugar composition of dissolved organic carbon (DOC) was investigated in four maritime Antarctic lakes on Signy Island of different trophic status; Heywood Lake (eutrophic), Light Lake (oligo-mesotrophic), Sombre Lake and Moss Lake (both oligotrophic). Tangential flow ultra-filtration (TFU) was used to separate DOC into high molecular weight (HMW; >1000 Da) and low molecular weight (LMW; < 1000 Da) size fractions. Pulsed amperometric detection-high performance liquid chromatography (PAD-HPLC) was used to determine the hydrolysable neutral sugar molecular composition of each size fraction. Total DOC concentrations defined the trophic trend in the four lakes and ranged from 8 to 303
μM. The <1000 Da fraction of all the lakes dominated the DOC distribution, comprising 76% in Light Lake which also had the highest chl- a concentrations. Heywood Lake was relatively enriched in >1000 Da total organic carbon and had extremely high concentrations of total hydrolysable neutral sugars (11 μM) corresponding to 43% of total DOC. However, no clear pattern was apparent with regard to lake trophic status and potential sources of DOC, and the measured variations in individual aldose concentration, composition and their various molecular weight fractions. Keywords dissolved neutral sugar carbohydrates dissolved organic carbon Signy Island eutrophic mesotrophic oligotrophic lakes References Allen, H. L. 1976 Dissolved organic matter in lake water: Characteristics of molecular weight size-fractions and ecological implications Oikos 27 64 70 Google Scholar Amon, R. M. W., Benner, R. 1994 Rapid cycling of high-molecular-weight dissolved organic matter in the ocean Nature 369 549 552 CrossRef Google Scholar Amon, R. M. W., Benner, R. 1996 Bacterial utilisation of different size classes of dissolved organic matter Limnol. Oceanogr. 41 41 51 CrossRef Google Scholar Azam, F. T., Fenchel, J. G., Feld, J. S., Gray, L. A., Meyer-Reil, X. X., Thingstad, F. 1983 The ecological role of water-column microbes in the sea Mar. Ecol. Prog. Ser. 10 257 263 Google Scholar
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