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Apoptosis

, Volume 24, Issue 5–6, pp 465–477 | Cite as

Drosophila jumu modulates apoptosis via a JNK-dependent pathway and is required for other processes in wing development

  • Xiao Chun Wang
  • Ziguang Liu
  • Li Hua JinEmail author
Article

Abstract

Previous studies in several model organisms have revealed that members of the Forkhead (Fkh) transcription factor family have multiple functions. Drosophila Jumeau (Jumu), a member of this family, participates in cardiogenesis, hematopoiesis and immune system homeostasis. Here, we show that loss of jumu function positively regulates or triggers apoptosis via a JNK-dependent pathway in wing development. jumu mutants showed reduced wing size and increased apoptosis. Moreover, we observed a loss of the anterior cross vein (ACV) phenotype that was similar to that observed in wings in which JNK signaling has been ectopically activated. The JNK signaling markers puckered (puc) and p-JNK were also significantly increased in the wing discs of jumu mutants. In addition, apoptosis induced by the loss of jumu was rescued by knocking down JNK, indicating a role for JNK in reducing jumu-induced apoptosis. Jumu could also control wing margin development via the positive regulation of cut expression, and the observed wing margin defect did not result from a loss of jumu-induced apoptosis. Further, jumu deficiency in the pupal wing could induce multiple wing hairs via a Rho1-mediated planar cell polarity pathway, but abnormal Rho1 expression was not why jumu loss induced apoptosis via a JNK-dependent pathway in wing discs.

Keywords

Jumu Apoptosis JNK pathway Wing development 

Notes

Acknowledgements

We thank Alan M Michelson for supplying us with the fly strains used in this study. We gratefully acknowledge Vienna Drosophila RNAi Stock Center, Tsinghua Drosophila model animal center, GenExel Stock Center and Developmental Studies Hybridoma Bank for providing fly lines and antibodies.

Author contributions

XCW, investigation, visualization, writing—original draft; LZ, review; LHJ, supervision, funding acquisition, project administration—review and editing.

Funding

This work was supported by the National Natural Science Foundation of China (31772521) and Fundamental Research Funds for the Central Universities (2572018CG05, 2572015AA10).

Compliance with ethical standards

Competing interests

The authors declare no competing or financial interests.

Supplementary material

10495_2019_1527_MOESM1_ESM.doc (5.3 mb)
Supplementary material 1 (DOC 5470 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Genetics, College of Life SciencesNortheast Forestry UniversityHarbinChina
  2. 2.Heilongjiang Academy of Agricultural SciencesHarbinChina

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