, Volume 24, Issue 1–2, pp 135–144 | Cite as

PPPDE1 promotes hepatocellular carcinoma development by negatively regulate p53 and apoptosis

  • Xingwang Xie
  • Xueyan Wang
  • Weijia Liao
  • Ran Fei
  • Nan Wu
  • Xu Cong
  • Qian Chen
  • Lai Wei
  • Yu WangEmail author
  • Hongsong ChenEmail author


We have previously identified that PPPDE1 is a deubiquitinase (DUB) belonging to a cysteine isopeptidase family. Here we sought to explore the biological significance of PPPDE1 in hepatocellular carcinoma and its underlying molecular mechanism. In the present study, we found that amplification and overexpression of PPPDE1 were associated with poor prognosis in hepatocellular carcinoma (HCC). We also demonstrated that knocking down of PPPDE1 could significantly block the clonal growth and tumorigenicity of human HCC cells, which revealed a critical role for PPPDE1 in HCC development. Furthermore, we proved that PPPDE1 is a key modulator of p53 protein level and its down stream apoptosis pathway. Taken together, these results suggested that PPPDE1 is a putative HCC driver gene and extensive studies should be conducted in the future to investigate the role of PPPDE1 in HCC and other tumors.


PPPDE1 Hepatocellular carcinoma Driver gene p53 Apoptosis 



Deubiquitinating enzymes


After Permuted Papain fold Peptidases of DsRNA viruses and Eukaryotes



This work was supported by grants from the National Natural Science Foundation of China (No. 81201569, No. 81541151), the Beijing Natural Science Foundation (No. 7132186) and the National Key Sci-Tech Special Project of China (No. 2018ZX10302207, No. 2017ZX10203202).

Compliance with ethical standards

Conflict of interest

There are no conflicts of interest to declare.

Supplementary material

10495_2018_1491_MOESM1_ESM.docx (92 kb)
Supplementary material 1 (DOCX 92 KB)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Xingwang Xie
    • 1
    • 2
  • Xueyan Wang
    • 2
  • Weijia Liao
    • 3
  • Ran Fei
    • 2
  • Nan Wu
    • 2
  • Xu Cong
    • 2
  • Qian Chen
    • 3
  • Lai Wei
    • 2
  • Yu Wang
    • 1
    Email author
  • Hongsong Chen
    • 2
    Email author
  1. 1.Chinese Center for Disease Control and PreventionBeijingChina
  2. 2.Peking University People’s Hospital, Peking University Hepatology InstituteBeijing Key Laboratory of Hepatitis C and Immunotherapy for Liver DiseaseBeijingChina
  3. 3.Laboratory of Hepatobiliary and Pancreatic SurgeryAffiliated Hospital of Guilin Medical UniversityGuilinChina

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