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Apoptosis

, Volume 22, Issue 5, pp 662–671 | Cite as

Remifentanil postconditioning ameliorates histone H3 acetylation modification in H9c2 cardiomyoblasts after hypoxia/reoxygenation via attenuating endoplasmic reticulum stress

  • Manli Chen
  • Qin Liu
  • Lijian Chen
  • Lei Zhang
  • Erwei GuEmail author
Article

Abstract

Remifentanil postconditioning (RPC) elicits cardioprotection against ischemia/reperfusion injury (IRI) by attenuating apoptosis associated with endoplasmic reticulum stress (ERS). Histone H3, acetylation modifications of histone H3, and histone deacetylases (HDAC) also have key roles in the mediation of the survival and apoptosis of cardiomyocytes. In this study, an in vitro IRI model was established with H9c2 cardiomyoblasts to investigate the role of histone H3 acetylation and HDAC3 in RPC-induced attenuation of ERS-associated apoptosis. Briefly, H9c2 cardiomyoblasts were randomly subjected to hypoxia/reoxygenation with and without remifentanil administered at the onset of reoxygenation. Results showed that RPC increased cell viability and prevented cell apoptosis (evidenced by CCK-8 cell viability assays and flow cytometry), and these effects were accompanied by lower levels of expression of GRP78, CHOP, cleaved caspase-12, and cleaved caspase-3. RPC also mimicked the effects of SAHA by increasing the amount of histone H3 deacetylation and decreasing up-regulation of HDAC at both the mRNA and protein levels in response to HR. Finally, RPC-induced protective effects against HR, including attenuation of ERS-associated protein markers, deacetylation of histone H3, and down-regulation of HDAC3 were completely abolished by pretreatment with thapsigargin (TG, a specific ERS activator). In contrast, these effects were not found to be enhanced after pretreatment with 4-phenyl butyric acid (4-PBA, a widely used ERS inhibitor). The present results demonstrate that RPC protects H9c2 cardiomyoblasts from HR injury, and this protection involves an attenuation of ERS-associated apoptosis, which mediates a reduction in HDAC3 expression and an increase in histone H3 deacetylation.

Keywords

Cardioprotection Remifentanil postconditioning Endoplasmic reticulum stress Histone 3 HDAC3 Apoptosis 

Notes

Acknowledgements

We thank LetPub (http://www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

Authors contributions

EG and MC designed experiments; MC and QL carried out experiments; LC analyzed experimental results. LZ analyzed sequencing data and developed analysis tools. MC wrote the manuscript and EG approved the final manuscript.

Funding

The funding was provided by the Key Research Project of education department of Anhui Province (No. KJ2013A161) and the Natural Science Foundation of China (No. 81341014).

Supplementary material

10495_2017_1347_MOESM1_ESM.tif (200 mb)
Supplementary material 1 (TIF 204823 KB)
10495_2017_1347_MOESM2_ESM.tif (188.9 mb)
Supplementary material 2 (TIF 193431 KB)

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Manli Chen
    • 1
    • 2
  • Qin Liu
    • 2
  • Lijian Chen
    • 2
  • Lei Zhang
    • 2
  • Erwei Gu
    • 2
    Email author
  1. 1.Department of AnesthesiologyThe First Affiliated Hospital, Medical School of Zhejiang UniversityHangzhouChina
  2. 2.Department of AnesthesiologyThe First Affiliated Hospital of Anhui Medical UniversityHefeiChina

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