, Volume 20, Issue 3, pp 273–284 | Cite as

Lysophosphatidic acid rescues bone mesenchymal stem cells from hydrogen peroxide-induced apoptosis

  • Xian-Yun Wang
  • Xue-Song Fan
  • Lin Cai
  • Si Liu
  • Xiang-Feng Cong
  • Xi ChenEmail author
Original Paper


The increase of reactive oxygen species in infracted heart significantly reduces the survival of donor mesenchymal stem cells, thereby attenuating the therapeutic efficacy for myocardial infarction. In our previous study, we demonstrated that lysophosphatidic acid (LPA) protects bone marrow-derived mesenchymal stem cells (BMSCs) against hypoxia and serum deprivation-induced apoptosis. However, whether LPA protects BMSCs from H2O2-induced apoptosis was not examined. In this study, we report that H2O2 induces rat BMSC apoptosis whereas LPA pre-treatment effectively protects BMSCs from H2O2-induced apoptosis. LPA protection of BMSC from the induced apoptosis is mediated mostly through LPA3 receptor. Furthermore, we found that membrane G protein Gi2 and Gi3 are involved in LPA-elicited anti-apoptotic effects through activation of ERK1/2- and PI3 K-pathways. Additionally, H2O2 increases levels of type II of light chain 3B (LC3B II), an autophagy marker, and H2O2-induced autophagy thus protected BMSCs from apoptosis. LPA further increases the expression of LC3B II in the presence of H2O2. In contrast, autophagy flux inhibitor bafilomycin A1 has no effect on LPA’s protection of BMSC from H2O2-induced apoptosis. Taken together, our data suggest that LPA rescues H2O2-induced apoptosis mainly by interacting with Gi-coupled LPA3, resulting activation of the ERK1/2- and PI3 K/AKT-pathways and inhibition caspase-3 cleavage, and LPA protection of BMSCs against the apoptosis is independent of it induced autophagy.


Bone marrow-mesenchymal stem cells (BMSCs) Lysophosphatidic acid (LPA) Hydrogen peroxide (H2O2Apoptosis Autophagy 



The Project was supported by the National Natural Science Foundation of China (30871024 and 81170154). The authors are indebted to Dr. Shi-Yuan Cheng for an extensive edit on the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10495_2014_1074_MOESM1_ESM.pdf (1.6 mb)
Supplementary material 1 (PDF 1611 kb)


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Xian-Yun Wang
    • 1
  • Xue-Song Fan
    • 1
  • Lin Cai
    • 1
  • Si Liu
    • 1
  • Xiang-Feng Cong
    • 1
  • Xi Chen
    • 1
    Email author
  1. 1.State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Centre for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

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