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Apoptosis

, Volume 19, Issue 3, pp 436–450 | Cite as

Different routes lead to apoptosis in unfertilized sea urchin eggs

  • Laetitia Philippe
  • Lucie Tosca
  • Wen Ling Zhang
  • Marion Piquemal
  • Brigitte CiapaEmail author
Original Paper

Abstract

Results obtained in various species, from mammals to invertebrates, show that arrest in the cell cycle of mature oocytes is due to a high ERK activity. Apoptosis is stimulated in these oocytes if fertilization does not occur. Our previous data suggest that apoptosis of unfertilized sea urchin eggs is the consequence of an aberrant short attempt of development that occurs if ERK is inactivated. They contradict those obtained in starfish, another echinoderm, where inactivation of ERK delays apoptosis of aging mature oocytes that are nevertheless arrested at G1 of the cell cycle as in the sea urchin. This suggests that the cell death pathway that can be activated in unfertilized eggs is not the same in sea urchin and in starfish. In the present study, we find that protein synthesis is necessary for the survival of unfertilized sea urchin eggs, contrary to starfish. We also compare the effects induced by Emetine, an inhibitor of protein synthesis, with those triggered by Staurosporine, a non specific inhibitor of protein kinase that is widely used to induce apoptosis in many types of cells. Our results indicate that the unfertilized sea urchin egg contain different mechanisms capable of leading to apoptosis and that rely or not on changes in ERK activity, acidity of intracellular organelles or intracellular Ca and pH. We discuss the validity of some methods to investigate cell death such as measurements of caspase activation with the fluorescent caspase indicator FITC-VAD-fmk or acidification of intracellular organelles, methods that may lead to erroneous conclusions at least in the sea urchin model.

Keywords

Apoptosis Egg Sea urchin MAP kinase Calcium Staurosporine 

Notes

Acknowledgments

We thank R. Dumollard and A. Mc Dougall for their help in Ca measurements and C. Billam for correcting our manuscript.

Supplementary material

Supplementary material 1 (WMV 1097 kb)

Supplementary material 2 (WMV 1108 kb)

10495_2013_950_MOESM3_ESM.docx (10 kb)
Supplementary material 3 (DOCX 10 kb)

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Copyright information

© European Union 2013

Authors and Affiliations

  • Laetitia Philippe
    • 1
  • Lucie Tosca
    • 2
  • Wen Ling Zhang
    • 3
  • Marion Piquemal
    • 1
  • Brigitte Ciapa
    • 1
    Email author
  1. 1.Centre de Neurosciences Paris-Sud (CNPS)Orsay CedexFrance
  2. 2.INSERM U935/Université Paris Sud/AP-HP, Histologie-Embryologie-CytogénétiqueHôpital Antoine BéclèreClamartFrance
  3. 3.Institute for Reproductive MedicineTorontoCanada

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