Apoptosis

, Volume 17, Issue 6, pp 539–550

Apoptosis, necrosis and autophagy are influenced by metabolic energy sources in cultured rat spermatocytes

  • Ximena Bustamante-Marín
  • Clara Quiroga
  • Sergio Lavandero
  • Juan G. Reyes
  • Ricardo D. Moreno
Original Paper

Abstract

Apoptosis, necrosis and autophagy are mechanistically related processes that control tissue homeostasis and cell survival. In the testis, germ cell death is important for controlling sperm output, but it is unknown whether or not germ cells can switch from apoptosis to necrosis, as has been reported in other tissues. Furthermore, autophagy has not been reported in spermatogenesis. Spermatocytes (meiotic cells) and spermatids (haploid cells) use lactate rather than glucose as their primary substrate for producing ATP. The metabolism of glucose, but not lactate, reduces ATP levels and increases intracellular [H+] and [Ca2+], both of which are associated with apoptosis and/or necrosis in somatic cells. In this work, we evaluated whether different energy sources, such as lactate or glucose, can influence spermatocyte death type and/or survival in primary cultures. Spermatocytes cultured for 12 h without an energy source died by necrosis, while spermatocytes cultured with 5 mM glucose showed a significant increase in apoptosis, as evidenced by caspase activity, TUNEL assay and phosphatidylserine exposure. Apoptosis was not observed in spermatocytes cultured with 5 mM lactate or deoxyglucose. Authophagy markers, such as LC3-II and autophagosomes, were detected after 12 h of culture, regardless the culture conditions. These results suggest that the availability of glucose and/or lactate affect the type of death or the survival of primary spermatocytes, where glucose can induce apoptosis, while lactate is a protective factor.

Keywords

Spermatogenesis Energetic metabolism Cell death Caspase Calcium 

Supplementary material

10495_2012_709_MOESM1_ESM.doc (214 kb)
Supplementary material 1 (DOC 214 kb)

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ximena Bustamante-Marín
    • 1
    • 5
  • Clara Quiroga
    • 2
    • 3
  • Sergio Lavandero
    • 2
    • 3
  • Juan G. Reyes
    • 4
  • Ricardo D. Moreno
    • 1
  1. 1.Departamento de Fisiología, Facultad de Ciencias BiológicasPontificia Universidad Católica de ChileSantiagoChile
  2. 2.Centro Estudios Moleculares de la Celula, Facultad Ciencias Quimicas y Farmaceuticas/Facultad MedicinaUniversidad de ChileSantiagoChile
  3. 3.Department of Internal Medicine (Cardiology Division)University of Texas Southwestern Medical CenterDallasUSA
  4. 4.Instituto de Química, Pontificia Universidad Católica de ValparaísoValparaísoChile
  5. 5.Departamento Biomédico, Facultad de Ciencias de la SaludUniversidad de AntofagastaAntofagastaChile

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