, Volume 15, Issue 5, pp 527–540 | Cite as

Regulation of apoptosis-associated lysosomal membrane permeabilization

  • Ann-Charlotte JohanssonEmail author
  • Hanna Appelqvist
  • Cathrine Nilsson
  • Katarina Kågedal
  • Karin Roberg
  • Karin Öllinger
Open Access
Original Paper


Lysosomal membrane permeabilization (LMP) occurs in response to a large variety of cell death stimuli causing release of cathepsins from the lysosomal lumen into the cytosol where they participate in apoptosis signaling. In some settings, apoptosis induction is dependent on an early release of cathepsins, while under other circumstances LMP occurs late in the cell death process and contributes to amplification of the death signal. The mechanism underlying LMP is still incompletely understood; however, a growing body of evidence suggests that LMP may be governed by several distinct mechanisms that are likely engaged in a death stimulus- and cell-type-dependent fashion. In this review, factors contributing to permeabilization of the lysosomal membrane including reactive oxygen species, lysosomal membrane lipid composition, proteases, p53, and Bcl-2 family proteins, are described. Potential mechanisms to safeguard lysosomal integrity and confer resistance to lysosome-dependent cell death are also discussed.


Lysosome Lysosomal release Caspases Calpains Hsp LAMP 



Aryl hydrocarbon receptor


Adenine nucleotide translocator


Apolipoprotein E


Bcl-2 homology 3


Heat shock protein


c-Jun N-terminal kinase


Lysosome-associated membrane protein


Lysosome-associated apoptosis-inducing protein containing the pleckstrin homology and FYVE domains


Lens epithelium-derived growth factor


Lysosomal membrane permeabilization




Phospholipase A2


Reactive oxygen species


Small-interfering RNA




Tumor necrosis factor


Tumor necrosis factor-related apoptosis inducing ligand




Over the last decade, the lysosome has emerged as a significant component of the cellular death machinery. Lysosomes, which were first described by de Duve and colleagues in 1955 [1], are acidic, single-membrane bound organelles that are present in all eukaryotic cells [2]. The primary function of lysosomes is degradation of macromolecules, and for this purpose, lysosomes are filled with more than 50 acid hydrolases, including phosphatases, nucleases, glycosidases, proteases, peptidases, sulphatases, and lipases [2]. Of the lysosomal hydrolases, the cathepsin family of proteases is the best characterized. The cathepsins are subdivided, according to their active site amino acids, into cysteine (cathepsins B, C, F, H, K, L, O, S, V, W, and X), serine (cathepsins A and G), and aspartic cathepsins (cathepsins D and E) [3].

Cell death through apoptosis is tightly controlled by changes in protein expression, protein-protein interactions, and various post-translational modifications, including proteolytic cleavage and phosphorylation. For some of these events to occur, proteins must re-localize from one sub-cellular compartment to another to gain access to their substrates or interacting partners. Thus, compartmentalization is yet another important regulatory mechanism, preventing accidental triggering of cell death signaling. The most obvious example is the release of apoptogenic factors from mitochondria, a key event in the execution of cell death, which is regulated mainly by proteins of the Bcl-2 family. Lysosomal hydrolytic enzymes were initially thought to inevitably trigger necrotic cell death when released into the cytosol [4]. However, in 1998, we discovered that the aspartic protease cathepsin D was redistributed from lysosomes to the cytosol upon oxidative stress-induced apoptotic cell death [5] (Fig. 1). Cathepsin D was the first identified lysosomal protein with apoptogenic properties, but the subcellular location of the protein in dying cells was not addressed in the early publications [6, 7]. In a series of papers from Brunk and co-workers, it was established that the extent of lysosomal damage determines the cell fate; a limited lysosomal release results in cell death by apoptosis, while massive lysosomal break-down leads to necrosis [8, 9, 10, 11, 12].
Fig. 1

Apoptosis-associated release of lysosomal cathepsin D into the cytosol. Visualization of cathepsin D in rat cardiomyocytes exposed to the redox-cycling drug naphthazarin by immunofluorescence microscopy (a and b) or immuno-electron microscopy using antibodies tagged with ultra-small gold particles and subsequent silver enhancement (c and d). There is a shift from a punctate lysosomal staining pattern in control cells (a) to a diffuse cytosolic staining in cells exposed to naphthazarin (b). Likewise, in electron micrographs (c), cathepsin D can be seen in lysosome-like structures in control cells (arrows), while in cells treated with naphthazarin (d), cathepsin D is spread throughout the cytosol (arrow heads), bars = 30 μm (a and b) and 1 μm (c and d)

It is now generally accepted that apoptosis is often associated with release of cathepsins into the cytosol, and that, in addition to cathepsin D, cysteine cathepsins B and L are involved in apoptosis signaling [13, 14, 15, 16, 17]. Moreover, lysosomal destabilization, which will subsequently be referred to as lysosomal membrane permeabilization (LMP), may also occur during other types of cell death, including necroptosis and autophagic cell death [18, 19]. LMP can be triggered by a wide range of apoptotic stimuli, including death receptor activation, endoplasmic reticulum stress, proteasome inhibition, oxidative stress, DNA damage, osmotic stress, and growth factor starvation [13, 14, 15, 16, 17]. In some experimental settings, lysosomal release is an early event essential for apoptosis signaling to proceed, while under other circumstances LMP occurs late in the cell death process and contributes to amplification of the death signal (Fig. 2). Cathepsin relocalization seems to be critical for apoptogenicity, and it has been demonstrated that microinjection of cathepsins into the cytosol is sufficient to trigger apoptotic cell death [20, 21, 22]. Cathepsin release may result in caspase-dependent or -independent cell death with or without involvement of mitochondria [13, 17]. Caspases-2 and -8, phospholipase A2 (PLA2), sphingosine kinase-1, and the Bcl-2 family proteins Bid, Bcl-2, Bcl-XL, and Mcl-1 may all be downstream targets of cathepsins. A detailed description of the mechanisms by which lysosomal cathepsins propagate the apoptotic signal can be found elsewhere [13, 14, 15, 16].
Fig. 2

Lysosomal participation in cell death signaling. Lysosomal membrane permeabilization (LMP), i.e., release of cathepsins from lysosomes into the cytosol, is an important step in cell death signaling induced by a variety of stimuli, such as death receptor activation, radiation, and cytotoxic drugs. Several cytosolic cathepsin targets have been described and the signaling downstream LMP involves both caspase-dependent and -independent pathways. Engagement of the mitochondrial pathway is a common downstream event of LMP, but cathepsins may also cause cell death without involvement of mitochondria

It is still unclear whether a subset of lysosomes is particularly susceptible to apoptosis-associated LMP and thus release all of their contents while other lysosomes release none, or if all lysosomes within a given cell are equally affected but release only part of their contents. There are, however, indications that interlysosomal differences regarding vulnerability to LMP do exist [23, 24]. For example, large lysosomes appears to be more susceptible to LMP than small ones [24].

The mechanism underlying LMP is still incompletely understood. Apart from cathepsins, other hydrolases [25, 26, 27, 28], H+ (causing acidification of the cytosol) [29], Ca2+ [30], and several of the dyes that accumulate in lysosomes with intact membranes, e.g., acridine orange [8], Lucifer Yellow [10], and LysoTracker [31], can escape into the cytosol at the onset of apoptosis. This may suggests a nonspecific release mechanism, such as pore formation or limited membrane damage, rather than selective transport across the membrane, which would likely be specific for a single group of molecules. However, it should be noted that the release of H+, Ca2+, and lysosomotropic dyes does not necessarily reflect a change in membrane integrity, but could merely be due to a change in ion pump activity. It has been suggested that apoptosis-associated lysosomal release is size-selective, as 10- and 40-kDa FITC-dextran molecules are released from lysosomes during staurosporine-induced T-cell apoptosis, while 70- and 250-kDa FITC-dextran molecules are retained [32]. Nevertheless, that upper limit of size-selection does not apply in all death models, as release of the 150 kDa lysosomal protein N-acetyl-β-glucosaminidase has been observed under other experimental conditions [25, 26, 27]. Conceivably, LMP may be governed by several distinct mechanisms, each with its own characteristics, which are likely engaged in a death stimulus- and cell-type-dependent fashion. The mechanisms that have been suggested to contribute to permeabilization of the lysosomal membrane are described in the following sections, as are those that are thought to safeguard lysosomal integrity and confer resistance to lysosome-dependent cell death.

Promoters of lysosomal membrane permeabilization

Viral and bacterial proteins

Apoptosis triggered by bacterial and viral infections sometimes involves LMP, and in certain contexts the participation of cathepsins is required for apoptotic signaling to proceed [33, 34, 35, 36, 37, 38]. The human immunodeficiency virus type 1 (HIV-1) protein Nef is one of the non-mammalian proteins that, when entering mammalian cells, causes permeabilization of the lysosomal membrane [36]. Overexpression of Nef elicits an apoptotic response in CD4+ T lymphocytes via disruption of lysosomal integrity, thus, the progressive and massive destruction of CD4+ T lymphocytes characteristic of HIV-1-related disease may in part be due to Nef-induced LMP. Parvovirus H1 is another virus that induces cell death characterized by translocation of cathepsins to the cytosol [34]. A small amount of viral proteins were found within the lysosomal fraction, and it was speculated that the capsid protein VP1, which possesses a domain displaying phospholipase A2 (PLA2) activity, could be involved in induction of LMP.

The diphtheria toxin is one of the most interesting examples of a bacterial protein that can permeabilize the membranes of the endolysosomal compartment. After entering the cell via endocytosis, one of the subdomains of the toxin inserts into the endosomal membrane and forms pores through which the catalytic subunit of the toxin passes into the cytosol [39, 40, 41, 42]. In the same way, components of the tetanus [43], botulinum [44], and anthrax [45] toxins are believed to permeabilize the endosomal membrane to gain access to the cytosol. As discussed below, certain mammalian proteins involved in apoptosis signaling share high sequence homology with these viral proteins, and may well be key regulators of apoptosis-associated LMP.

Pro-apoptotic Bcl-2 family proteins

The Bcl-2 family consists of both pro- (e.g., Bax, Bak, Bid, Bad, Bim, Noxa, and Puma) and anti-apoptotic (e.g., Bcl-2, Bcl-XL, and Mcl-1) proteins that are critical regulators of the mitochondrial pathway to cell death. The multi-domain proteins Bax and Bak are pore-forming proteins that enable the release of apoptogenic factors such as cytochrome c from mitochondria, while the so-called BH3-only-domain proteins (e.g., Bid, Bad, Bim, Noxa, and Puma) serve to promote activation of these multi-domain family members upon an apoptotic insult. The main function of the anti-apoptotic Bcl-2 family proteins, on the other hand, is to prevent the release of apoptogenic factors. Interestingly, the hypothesis that Bcl-2 family proteins can release cytochrome c through pore formation was based on the discovery that some of these proteins showed high structural similarity to the pore-forming domain of the diphtheria toxin, which, as mentioned above, is known to form pores in the endosomal membrane [46, 47]. Several years later, we discovered that Bax could also permeabilize the lysosomal membrane, and thereby promote cell death by the release of lysosomal cathepsins to the cytosol [26]. Confocal and immuno-electron microscopy showed that Bax translocated from the cytosol to the lysosomal membrane upon staurosporine treatment of human fibroblasts. Furthermore, recombinant Bax was inserted into the membrane of isolated rat liver lysosomes and caused the release of lysosomal constituents. These data provided the first evidence that Bax is mechanistically involved in LMP and are now supported by a growing number of publications demonstrating involvement of multiple pro- and anti-apoptotic Bcl-2 family proteins in the regulation of lysosomal membrane integrity.

Bax is activated and relocated to lysosomes in hepatocytes upon treatment with free fatty acids [48, 49], in cholangiocarcinoma cells in response to tumor necrosis factor-related apoptosis inducing ligand (TRAIL) [50], and in ovarian cancer cells treated with etoposide [51]. In all three cases, small interfering RNA- (siRNA)-mediated downregulation of Bax prevented LMP, indicating a mechanistic importance of Bax’s lysosomal location. Furthermore, siRNA-mediated downregulation of Bim was found to prevent TRAIL-induced LMP [50]. Bim was activated upon TRAIL treatment and colocalized with Bax in lysosomes, suggesting that it may trigger Bax-mediated LMP. In another study, Bid, the most well-known Bax activator, was found to be involved in tumor necrosis factor (TNF)-α-induced lysosomal destabilization in hepatocytes [52]. Upon TNF-α treatment, Bid was detected in lysosomes, and cathepsin B was released to the cytosol. However, no such release was seen in Bid−/− cells. Even though the involvement of Bax was not considered in this study, it is tempting to speculate that LMP was caused by Bid-mediated activation of Bax. In a recent report, BH3 domain peptides were found to permeabilize isolated lysosomes in the presence of Bax [50]. Interestingly, Bak, the other main pore-forming Bcl-2 family protein, also localizes to lysosomes [48], but there are as yet no reports of Bak-induced LMP. Conceivably, triggering of LMP could be a general function of pro-apoptotic Bcl-2 proteins. Similar to mitochondrial membrane permeabilization [53], LMP-associated Bax activation seems to rely on different BH3-only proteins depending on the death stimulus and cell type. In addition to a direct effect of Bcl-2 proteins at the lysosomal membrane, Bcl-2 family members may regulate LMP at the mitochondrial level as engagement of mitochondria can lead to increased lysosomal release via positive feed-back. However, as release of cathepsins has been observed in Bax/Bak double knockout cells there are clearly additional mechanisms independent of Bax and Bak that contribute to LMP [54].


Permeabilization of the lysosomal membrane occurs in p53-induced apoptosis [55], during which p53 itself localizes to lysosomes and triggers LMP in a transcription-independent manner [56]. It was proposed that p53 phosphorylated at Ser15 (p-p53Ser15) may be recruited to the lysosomal membrane by a newly discovered protein called Lysosome-associated apoptosis-inducing protein containing the pleckstrin homology and FYVE domains (LAPF). This protein, which associates with lysosomes upon induction of apoptosis, is essential for cell death induced by TNF-α, ionizing irradiation, and the DNA-damaging drugs 5-fluorouracil and oxaliplatin [56, 57]. Downregulation of either p53 or LAPF prevented TNF-α-induced LMP [56]. Interestingly, silencing of LAPF expression abrogated lysosomal translocation of p-p53Ser15, whereas silencing of p53 expression had no effect on lysosomal translocation of LAPF, indicating that LAPF may trigger LMP by acting as an adaptor protein for p-p53Ser15. Furthermore, p-p53Ser15 localizes to lysosomes in cultured cortical neurons after exposure to the psychoactive ingredient of marijuana, ∆9-tetrahydrocannabiol [58], or β-amyloid [59], both of which cause neuronal cell death. Also in these models of apoptosis, LMP was abrogated by siRNA-mediated downregulation or chemical inhibition of p53. It is worth noting that p53, under certain circumstances, localizes to the mitochondrial membrane during apoptosis and can trigger mitochondrial membrane permeabilization by direct interaction with Bcl-2 family members [60, 61]. It is tempting to speculate that a similar mechanism governs p53-induced LMP. Additionally, p53 promotes Bax-mediated mitochondrial membrane permeabilization through transcriptional upregulation of the BH3-only-domain proteins Puma and Noxa [62, 63, 64]. In this way, p53 may engage the lysosomal pathway without translocating to lysosomes. Recent data from our lab supports this notion; ultraviolet B- (UVB)-induced, p53-dependent LMP is associated with an increase in Puma and Noxa expression without the appearance of p53 at the lysosomal membrane [65].



There are a number of publications suggesting that caspases, under certain circumstances, are involved in the release of apoptogenic factors from lysosomes [50, 52, 66, 67, 68, 69]. Caspase-8, in particular, plays a significant role in the engagement of the lysosomal death pathway following death receptor activation [50, 52, 67, 68, 69]. Caspase-8 can trigger the release of cathepsins from purified lysosomes, and the effect was enhanced by the addition of a cytosolic fraction [67], indicating that caspase-8-induced LMP is at least partly mediated by activation of one or several cytosolic substrates. Bid, which presumably would trigger Bax-mediated LMP, has been presented as a possible candidate [52]. In our hands, neither caspase-8 nor activated Bid alone permeabilized the membrane of isolated lysosomes [26], suggesting that additional factors, such as Bax or Bak, are indeed required. In another intriguing study, caspase-8 cleaved and activated caspase-9 in an apoptosome-independent fashion [68]. By using genetically modified mouse embryonic fibroblasts, caspase-9 was proven to be indispensable for triggering LMP, but the exact mode of action remains elusive. Interestingly, depletion of Bid did not prevent the loss of lysosomal membrane integrity. These data suggest that there are at least two different mechanisms by which caspase-8 is able to induce LMP.

In a recent study, caspase-2 activation was shown to be required for LMP during endoplasmic reticulum stress-induced apoptosis [70]. The effect was not dependent on Bax, ceramide, Ca2+, or reactive oxygen species (ROS) production, suggesting a more direct role of caspase-2. Indeed, caspase-2 has previously been shown to induce cathepsin release from purified lysosomes [67].


It has been proposed that lysosomal proteases, in particular cathepsin B, may contribute to their own release [31, 48, 71]. In mouse hepatocytes, lysosomal destabilization evoked by TNF-α and sphingosine is dependent on cathepsin B, as induction of LMP fails in cathepsin B-deficient cells [31]. Moreover, chemical inhibition of cathepsin B significantly reduces both free fatty acid- and TRAIL-induced LMP [48, 71]. However, it is not clear whether intra- or extralysosomal cathepsin B is involved. As cathepsin B is constitutively active inside lysosomes, it cannot be sufficient for LMP; however, it may be necessary. Data in favor of intralysosomal cathepsin B as a mediator of LMP was provided by cell-free experiments in which sphingosine was found to cause lysosomal release from cathepsin B-containing but not from cathepsin B-deficient lysosomes [31]. Alternatively, cathepsin B-mediated LMP may constitute an amplifying feedback loop, in which a small amount of released cathepsin B triggers more extensive LMP from outside the lysosome. The latter is supported by the finding that upregulation of serine protease inhibitor 2A, a potent inhibitor of cathepsin B located in the cytosol and nucleus, reduces lysosomal breakdown [72].


Calpains catalyze lysosomal membrane destabilization during neuronal cell death [73, 74, 75, 76]. Cell death induced by ischaemia or hypochloric acid is associated with an increase in cytosolic [Ca2+], activation of calpains, and LMP. Activated μ-calpain localizes to the lysosomal membrane prior to release of cathepsins, indicating involvement in the triggering of LMP [74, 75, 77]. In further support of this hypothesis, μ-calpain can permeabilize the membrane of isolated lysosomes [67], and pharmacological inhibition of calpains abrogates LMP [73]. Interestingly, calpain activity correlates with the appearance of p-p53Ser15 at the lysosomal membrane, suggesting that calpains may, under certain circumstances, promote p53-mediated lysosomal release [59].

Reactive oxygen species

An increased production of ROS may lead to destabilization of the lysosomal membrane via massive peroxidation of membrane lipids. Photosensitizers located in lysosomes that generate singlet oxygen upon radiation have been shown to cause rapid release of cathepsins to the cytosol [78, 79, 80]. Moreover, apoptosis induced by UVA radiation or H2O2, both of which trigger cell death mainly by generating oxidative stress, involves LMP [12, 65, 81]. Additionally, other apoptosis inducers, e.g., N-(4-hydroxyphenyl)retinamide [82] and vacuolar ATPase inhibitors [83], cause ROS-dependent LMP. However, in the latter model systems it is not clear whether leakage over the lysosomal membrane is due to ROS-mediated membrane damage. Alternatively, alterations in the intracellular redox balance may elicit a redox-dependent signaling cascade resulting in LMP.

Induction of LMP by lysosomal membrane peroxidation reactions has been extensively studied in model systems in which the intralysosomal iron content has been manipulated [84, 85, 86, 87, 88]. It is hypothesized that intralysosomal iron, originating from degraded metallo proteins, reacts with H2O2 and generates highly reactive hydroxyl radicals that initiate peroxidation of the lysosomal membrane (Fig. 3). In an illustrative experiment, macrophages that were allowed to phagocytose silica particles with high amounts of surface-bound iron suffered from extensive lysosomal damage, while those that ingested silica particles pretreated with the pharmacologic iron chelator desferrioxamine displayed only modest lysosomal leakage [89]. In several additional studies it has been established that phagocytosis of iron complexes or iron-containing proteins increases lysosomal vulnerability, while a reduction in intralysosomal reactive iron reduces lysosomal leakage and cell death [84, 85, 86, 87, 88].
Fig. 3

Destabilization of the lysosomal membrane by lipid peroxidation. In response to oxidative stress, increased amounts of hydrogen peroxide can diffuse into the lysosome. In the lysosomal lumen, the acidic milieu and the presence of low-molecular-weight iron, derived from degraded iron-containing proteins, promotes the reduction of iron and the generation of hydroxyl radicals. The hydroxyl radicals induce peroxidation of membrane lipids and thereby cause leakage of lysosomal constituents into the cytosol


Cellular signal transduction involves a wide range of small GTPases and kinases constituting a complex signaling network that ultimately regulates virtually all cellular events including cell proliferation, differentiation, and cell death. Ras signaling pathways participate in the overall regulation of lysosomal function, and phosphatidylinositol-3-kinase (PI3K), one of the downstream targets of Ras, is indispensable for vesicular transport to lysosomes [90, 91, 92]. H-ras driven transformation of NIH3T3 cells causes increased expression of cysteine cathepsins and reduced lysosomal stability during TNF-induced cell death [93]. Inhibition of PI3K sensitizes vascular endothelial cells to cytokine-induced apoptosis by increasing lysosomal permeability [94]. Furthermore, activation of c-Jun N-terminal kinase (JNK) promotes lysosomal breakdown in response to TNF-α, TRAIL, and UVB radiation, possibly via activation of the BH3-only-domain protein Bim [50, 95, 96]. Additionally, in neurons exposed to β-amyloid, phosphorylation of p53 at Ser15 is JNK1 dependent [97]. In contrast, inhibitors of JNK fail to affect lysosomal permeability in H-ras transformed NIH3T3 cells. Instead, activation of the Raf1-extracellular signal-regulated kinase (-ERK) pathway is responsible for increased engagement of the lysosomal death pathway [98]. Taken together, these data suggest that LMP is likely regulated by kinase-dependent signaling events, although the number of studies so far is limited.

Changes in lysosomal membrane lipid composition

Obviously, the lysosomal membrane composition plays a key role in the maintenance of lysosomal integrity. Damage to lysosomal membrane components or changes in the membrane structure and fluidity could result in lysosomal destabilization.

PLA2, phospholipase C, and sphingomyelinase, enzymes that regulate the membrane lipid composition, all show increased activity in the presence of high cytosolic [Ca2+], which is characteristic of apoptosis. Studies using isolated rat lysosomes have shown that these enzymes [99, 100, 101, 102] as well as the phospholipid products arachidonic acid [103], lysophosphatidylcholine [104], and phosphatidic acid [105], affect the osmotic sensitivity of lysosomes, making them more susceptible to osmotic stress.

PLA2 stands out as a particularly interesting candidate because it is involved in lysosomal membrane destabilization in several different experimental systems [30, 76, 106, 107, 108, 109]. For example, lysosomes are permeabilized in a PLA2-dependent fashion in response to low concentrations of H2O2 [106]. In this context, activation of PLA2 is dependent on an early minor release of lysosomal contents (suggesting an alternative mechanism for the initial release), and PLA2 engages in an amplifying feed-back loop. Notably, incubation of PLA2 with rat liver lysosomes results in leakage of lysosomal constituents.

Apoptosis-associated LMP may also be mediated by an increase in the sphingolipid sphingosine [10, 31]. Ceramide is produced from sphingomyelin upon activation of sphingomyelinases (SMase) and is further processed into sphingosine by ceramidase [110] (Fig. 4). It has been proposed that sphingosine accumulates inside lysosomes, where it permeabilizes the membrane in a detergent-like fashion, resulting in cell death [10]. Under certain circumstances, sphingosine-induced LMP is dependent on cathepsin B, as sphingosine fails to permeabilize lysosomes from cathepsin B−/− hepatocytes [31]. These data also present the possibility that sphingosine serves as a general trigger of cathepsin B-mediated intralysosomal events leading to LMP.
Fig. 4

Accumulation of sphingosine leads to lysosomal membrane permeabilization. An apoptotic stimulus, e.g., tumor necrosis factor-α, leads to increased activity of sphingomyelinase, which catalyzes the formation of ceramide from sphingomyelin. By the action of ceramidase, ceramide is then converted into sphingosine, which accumulates in lysosomes and acquires detergent-like properties, leading to lysosomal membrane permeabilization

In a recent publication, proteins upregulated in lysosomes at the onset of apoptosis-associated LMP were identified using a proteomic approach [111]. Interestingly, two of these proteins, prosaposin and protein kinase Cδ, can lead to activation of the lysosomal enzyme acid SMase. Protein kinase Cδ activates acid SMase after translocating from the cytosol to the lysosomal membrane [112], and prosaposin is the precursor of four lysosomal sphingolipid activator proteins (saposins A-D) that promote acid SMase-mediated sphingomyelin degradation [113].

Other factors


Alzheimer’s disease is an age-related progressive neurodegenerative disease in which β-amyloid peptides are thought to confer neurotoxicity. Accumulation of β-amyloid in lysosomes of cultured cells results in LMP-dependent cell death [114, 115]. As β-amyloid is amphipathic and forms micelle-like aggregates [116, 117], the toxicity of β-amyloid could be mechanistically similar to that of lysosomotropic detergents, which self-assemble into micelles within lysosomes [118]. Alternatively, β-amyloid, which, like some of the Bcl-2 family proteins, shows structural similarities to pore-forming bacterial toxins, may trigger lysosomal release by creating pores. Indeed, oligomeric β-amyloid can incorporate into artificial lipid bilayers and form pore-like structures [119, 120].

Apolipoprotein E

Apolipoprotein E (apoE) is involved in lipid transport and one of its three major isoforms, apoE4, is a known risk factor for Alzheimer’s disease. ApoE4-transfected cells are more susceptible to lysosomal leakage induced by β-amyloid as compared to untransfected cells or cells transfected with apoE3 [121]. This effect is dependent on a low intralysosomal pH, as overexpression of apoE4 fails to potentiate leakage from lysosomes neutralized with bafilomycin or NH4Cl. Furthermore, at acidic pH, apoE4 binds more avidly to phospholipid bilayer vesicles and potentiates β-amyloid-induced disruption to a greater extent. It was suggested that, especially at a low pH, apoE4 is converted into a molten globule capable of binding and altering membrane stability [122]. Thus, such apoE4-mediated effects on membrane stability are likely restricted to the lysosomal membrane, and may, under certain circumstances, play a significant role in the determination of cell fate.

Aryl hydrocarbon receptor

In a murine hepatoma cell line in which TNF-α-induced cell death was found to be independent of caspase-8, LMP was dependent on the aryl hydrocarbon receptor (Ahr) [123]. This receptor is a ligand-activated transcription factor whose target genes are involved in many cellular functions, including apoptosis. In addition, it has been suggested that the Ahr may have ligand-independent activities. Ahr deficiency prevents disruption of lysosomes induced not only by TNF-α treatment, but also following photodynamic therapy [124].


The adenine nucleotide translocator (ANT) agonist atractyloside, which triggers opening of the mitochondrial permeability transition pore and release of mitochondrial cytochrome c, was shown to also induce release of cathepsin B from isolated lysosomes [125]. This was accidentally discovered by researchers aiming to identify factors released from a mitochondrial fraction, apparently contaminated with lysosomes. This finding suggests that ANT-like proteins may be present in the lysosomal membrane and regulate its permeability; however, in our hands, only a very high concentration of atractyloside permeabilized the membrane of isolated rat liver lysosomes [26].


The killer effector molecule granulysin, which, together with perforin and granzymes, is located in the cytolytic granules of human cytotoxic T-lymphocytes and natural killer cells was recently found to cause necroptosis (programmed necrosis) via LMP [19]. Granulysin, which interacts with lipids to cause disruption of membranes, has a broad-spectrum antimicrobial function and shows potent cytotoxic activity against tumor cells. Granulysin enters lysosomes of tumor cells and triggers release of cathepsin B, which in turn engages the mitochondrial pathway via cleavage of Bid. Thus, it appears that our immune system is armed with a weapon to target lysosomes for the destruction of unwanted cells.

Safeguards of lysosomal membrane integrity

It has been suggested that the stability of the lysosomal membrane could be modulated in different ways, and that this could contribute to the determination of cell fate. An example from normal physiological conditions is the selection of B-lymphocytes in the germinal centers. B-lymphocytes with high affinity B-cell receptors are spared from apoptotic cell death by interaction with follicular dendritic cells, which confer resistance to LMP [69]. The underlying mechanism was not unraveled in this study, but molecules involved in the stabilization of the lysosomal membrane have been identified by others (see below). Furthermore, cancer cells often show various changes in lysosomal function as well as altered susceptibility to lysosome-mediated cell death. For example, activation of the Ras or Src oncogenes is associated with changes in the lysosomal distribution, density, size, ultrastructure, and content, all of which may influence membrane stability [24, 90, 91, 98].

Heat shock proteins

Heat shock proteins (Hsps) are molecular chaperones essential for cells’ ability to cope with environmental stress [126]. Interestingly, Hsp70 is found at the lysosomes of many tumor cells and stressed cells, and has been shown to prevent LMP induced by TNF-α, etoposide, H2O2, or UVB irradiation [27, 127, 128]. Notably, Hsp70 is frequently overexpressed in tumors, and downregulation of Hsp70 in breast, pancreatic, or colon cancer cells leads to LMP and cathepsin-mediated cell death without any external death stimuli [27, 129], suggesting that the tumorigenic potential of Hsp70 could, in part, be due to stabilization of lysosomes. Hsp70 may prevent lysosomal membrane permeabilization by scavenging of lysosomal free iron and blocking of oxidative events [128, 130]. Moreover, it is tempting to speculate that Hsp70, which has been shown to interact with Bax and prevent its translocation to mitochondria [131, 132], could interfere with Bax-induced LMP.

In addition, Hsp70-2 can prevent permeabilization of the lysosomal membrane [133]. In this case, the protective effect depends on Hsp70-2-mediated upregulation of lens epithelium-derived growth factor (LEDGF). Knockdown of LEDGF in cancer cells induces destabilization of lysosomal membranes and cathepsin-mediated cell death, while ectopic expression stabilizes lysosomes and prevents cancer cell death. However, as LEDGF has not been detected in lysosomes, the protective mechanism remains obscure.

Bcl-2 proteins

Anti-apoptotic members of the Bcl-2 family are well-known regulators of mitochondrial membrane permeabilization, which, during recent years, have gained attention as possible modulators of lysosomal membrane stability [49, 50, 66, 134, 135]. Our finding that LMP may be induced by Bax is supported by the fact that overexpression of Bcl-2 prevents lysosomal destabilization [66, 134, 135]. In two of these studies, it was suggested that Bcl-2 stabilizes lysosomes by preventing activation of PLA2 [134, 135]. However, it is tempting to speculate that the protective effect of Bcl-2 is, at least in part, due to neutralization of pro-apoptotic members of the Bcl-2 family. The theory that anti-apoptotic Bcl-2 proteins are safeguards of lysosomal membrane integrity is supported by data from Gregory Gores and co-workers [49, 50]. In these studies, Mcl-1 and Bcl-XL prevented LMP induced by TRAIL and free fatty acids, respectively. In both cases, Bax was activated and translocated from the cytosol to lysosomes upon apoptosis induction. Furthermore, overexpression of Mcl-1 and Bcl-XL, as well as siRNA-mediated downregulation of Bax, stabilized the lysosomal membrane.


As described previously, ROS may compromise the integrity of lysosomes via peroxidation of membrane lipids. A variety of protective mechanisms have evolved to scavenge ROS and protect cells from their adverse effects; these are collectively known as the cell’s antioxidant defense. The defense mechanisms include low-molecular-weight antioxidants, for example vitamins C and E, coenzyme Q10, and glutathione, as well as antioxidant enzymes, such as glutathione peroxidase, catalase, and superoxide dismutase [136]. Vitamin E, a lipid soluble antioxidant that protects from lipid peroxidation [137], can prevent lysosomal release [5, 138]. Moreover, LMP induced by N-(4-hydroxyphenyl) retinamide or inhibitors of the vacuolar ATPase can be abrogated by increasing the amount of intracellular cysteine, which alters the redox balance [82, 83].

Furthermore, iron-binding proteins may serve to mitigate oxidant-induced LMP, as intralysosomal free iron can generate highly reactive hydroxyl radicals, leading to peroxidation of membrane lipids. Indeed, a number of studies have demonstrated that iron-binding proteins such as ferritin and metallothionein can reduce lysosomal leakage and cell death [84, 85, 139]. As mentioned above, also Hsp70 can bind intralysosomal redox-active iron and may, in that way, protect the lysosomal membrane from free-radical attack and permeabilization [128, 130].

Cholesterol and sphingomyelin

Cholesterol may play an important role in the maintenance of lysosomal membrane stability. It has long been known that addition of cholesterol to lysosomes reduces their permeability [140]. Accordingly, a reduced lysosomal membrane cholesterol level is associated with increased permeability to protons and potassium ions; this, in turn, causes an osmotic imbalance and destabilization of the lysosomal membrane [141, 142, 143]. Sphingomyelin affects the membrane fluidity by acting as a scaffold for incorporation of cholesterol [144, 145]. Exogenous sphingomyelin, as well as the sphingomyelin derivative 3-O-methylsphingomyelin, have been shown to accumulate in the lysosomal membrane and protect from lysosomal membrane disruption in response to TNF-α and lysosomal photosensitizers [78]. Thus, apoptosis-associated conversion of sphingomyelin into sphingosine (Fig. 4), a promoter of LMP, appears to have a dual negative effect on lysosomal membrane integrity.

Lysosome-associated membrane protein-1 and -2

The lysosomal membrane contains highly glycosylated proteins whose complex intralumenal carbohydrate side chains form a coat on the inner surface of the membrane. It has been suggested that these glycoproteins serve as a barrier against the hydrolytic activity of the lysosomal enzymes and thereby prevent accidental release of lysosomal constituents into the cytosol. Lysosome-associated membrane protein-1 (LAMP)-1 and -2, which constitute ~50% of the lysosomal membrane proteins [146, 147], can modulate the sensitivity of the lysosomal membrane to an apoptotic insult [98]. Oncogene-induced reduction in LAMP-1 and -2 can be caused by cathepsin B overexpression and is associated with increased susceptibility to LMP in response to photo-oxidation and the anti-cancer drug siramesine. The authors of that finding speculate that upregulation of lamp-1 and -2 mRNA, which has been observed in various human cancers [148, 149], might be an attempt to compensate for the deleterious effect of the reduced half-life of LAMP-1 and -2 in cancer cells overexpressing lysosomal cysteine cathepsins.


A growing body of evidence suggests that apoptosis is generally associated with LMP, occurring either as a triggering early event or an amplifying late event. With the wider acceptance of lysosomes as part of the signaling cascade leading to apoptotic cell death, the molecular mechanisms governing LMP have received increased attention. As outlined above, there are many proteinaceous and non-proteinaceous factors, including Bcl-2 family proteins, ROS, caspases, cathepsins, cholesterol, and Hsps, that influence the stability of the lysosomal membrane, and their individual importance appears to depend on the cell type and death stimulus (Fig. 5). However, the main mechanism responsible for apoptosis-associated LMP, if any, remains to be identified. A deeper understanding of the regulation of LMP and other apoptosis signaling events could enable the development of novel therapies for diseases associated with excessive or insufficient apoptosis such as neurodegenerative disorders and cancer, respectively.
Fig. 5

Factors regulating lysosomal membrane permeabilization. This schematic presents a number of factors that may be responsible for lysosomal membrane permeabilization (LMP). The relative importance of each mechanism likely depends on the cell type and death stimulus. Mechanisms that are believed to safeguard lysosomal integrity and protect from lysosome-mediated cell death are also shown. “Changes in membrane lipid composition” includes membrane destabilizing factors such as phospholipase A2 and sphingosine, as well as LPM protective substances e.g., cholesterol and sphingomyelin. Abbreviations: JNK, c-Jun N-terminal kinase; LAPF, lysosome-associated apoptosis-inducing protein containing the pleckstrin homology and FYVE domains; Hsp, heat shock protein; ROS, reactive oxygen species; ANT, adenine nucleotide translocator; Ahr, aryl hydrocarbon receptor; LEDGF, lens epithelium-derived growth factor; LAMP, lysosome-associated membrane protein



We thank Lotta Hellström and Fredrik Jerhammar for critical reading of the manuscript.

Open Access

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.


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© The Author(s) 2010

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Authors and Affiliations

  • Ann-Charlotte Johansson
    • 1
    Email author
  • Hanna Appelqvist
    • 2
  • Cathrine Nilsson
    • 1
    • 2
  • Katarina Kågedal
    • 2
  • Karin Roberg
    • 1
    • 3
  • Karin Öllinger
    • 2
  1. 1.Division of OtorhinolaryngologyLinköping University HospitalLinköpingSweden
  2. 2.Division of Experimental Pathology, Department of Clinical and Experimental MedicineLinköping UniversityLinköpingSweden
  3. 3.Division of Otorhinolaryngology, Department of Clinical and Experimental MedicineLinköping UniversityLinköpingSweden

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