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Apoptosis

, Volume 11, Issue 8, pp 1439–1451 | Cite as

Mechanisms of cell death induction by L-amino acid oxidase, a major component of ophidian venom

  • Sudharsana Rao Ande
  • Phaneeswara Rao Kommoju
  • Sigrid Draxl
  • Michael Murkovic
  • Peter Macheroux
  • Sandro Ghisla
  • Elisa Ferrando-May
Article

Abstract

L-amino acid oxidase (LAAO) from the Malayan pit viper induces both necrosis and apoptosis in Jurkat cells. Cell death by necrosis is attributed to H2O2 produced by oxidation of α-amino acids. In the presence of catalase that effectively scavenges H2O2, a switch to apoptosis is observed. The major factors contributing to apoptosis are proposed to be: (i) generation of toxic intermediates from fetal calf serum (ii) binding and internalization of LAAO. The latter process appears to be mediated by the glycan moiety of the enzyme as desialylation reduces cytotoxicity. D-amino acid oxidase (DAAO), which catalyzes the same reaction as LAAO but lacks glycosylation, triggers necrosis as a consequence of H2O2 production but not apoptosis in the presence of catalase. Thus induction of cell death by LAAO appears to involve both the generation of H2O2 and the molecular interaction of the glycan moiety of the enzyme with structures at the cell surface.

Keywords

Amino acids Apoptosis Flavin Glycosylation Necrosis 

Abbreviations

AIP

Apoptosis-inducing protein

DAAO

D-Amino acid oxidase

LAAO

L-Amino acid oxidase

zVAD-fmk

benzyloxycarbonyl-Val-Ala-Asp-CH2OC (O)-2,6 dichloro benzene, fluoro methyl ketone

DEVD-AFC

Asp-Glu-Val-Asp 7-amino-4-trifluoromethyl coumarin

FCS

Fetal calf serum

Lpt-medium

LAAO pretreated-medium

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Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Sudharsana Rao Ande
    • 1
  • Phaneeswara Rao Kommoju
    • 1
  • Sigrid Draxl
    • 3
  • Michael Murkovic
    • 3
  • Peter Macheroux
    • 4
  • Sandro Ghisla
    • 1
  • Elisa Ferrando-May
    • 2
  1. 1.Biochemistry Research GroupUniversity of KonstanzKonstanzGermany
  2. 2.Molecular Toxicology Group, Department of BiologyUniversity of KonstanzKonstanzGermany
  3. 3.Institute of Food Chemistry and TechnologyGraz University of TechnologyGrazAustria
  4. 4.Institute of BiochemistryGraz University of TechnologyGrazAustria

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