Apoptosis

, Volume 11, Issue 7, pp 1247–1255 | Cite as

Dexamethasone inhibits apoptosis in C6 glioma cells through increased expression of Bcl-XL

  • Tríona Ní Chonghaile
  • Caoimhín G. Concannon
  • Eva Szegezdi
  • Adrienne M. Gorman
  • Afshin Samali
Reports

Abstract

The glucocorticoid dexamethasone (Dex) has been reported to modulate a number of signaling pathways and physiological processes, including apoptosis. This study was carried out to investigate the cytoprotective mechanism of Dex in C6 glioma cells. Pre-treatment of cells with Dex inhibited apoptosis induced by staurosporine, etoposide and thapsigargin. Apoptosis inhibition correlated with blockade of mitochondrial cytochrome c release, abolition of caspase-3 activity along with inhibition of caspase-9 and PARP cleavage. Dex-mediated cytoprotection coincided with the induction of the anti-apoptotic protein, Bcl-XL. The specific glucocorticoid receptor antagonist, RU486, reversed the anti-apoptotic effect of Dex and prevented Bcl-XL induction. Here, we show for the first time that knockdown of Bcl-XL expression with siRNA reversed the protective effects of the glucocorticoid in glioma cells. We conclude that Dex-mediated inhibition of apoptosis in C6 glioma cells is through induction of Bcl-XL.

Keywords

Apoptosis Bcl-XL Dexamethasone Glioma siRNA 

Abbreviations

Dex

Dexamethasone

GAPDH

Glyceraldehyde 3-phosphate dehydrogenase

GR

Glucocorticoid receptor

GRE

Glucocorticoid response element

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Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Tríona Ní Chonghaile
    • 1
  • Caoimhín G. Concannon
    • 1
  • Eva Szegezdi
    • 1
  • Adrienne M. Gorman
    • 1
  • Afshin Samali
    • 2
  1. 1.Department of Biochemistry and National Centre for Biomedical Engineering ScienceNational University of IrelandGalwayIreland
  2. 2.Cell Stress and Apoptosis Research GroupDepartment of Biochemistry, NUIGalwayIreland

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