NMR exposure sensitizes tumor cells to apoptosis
- 116 Downloads
NMR technology has dramatically contributed to the revolution of image diagnostic. NMR apparatuses use combinations of microwaves over a homogeneous strong (1 Tesla) static magnetic field. We had previously shown that low intensity (0.3–66 mT) static magnetic fields deeply affect apoptosis in a Ca2+ dependent fashion (Fanelli et al., 1999 FASEBJ., 13;95–102). The rationale of the present study is to examine whether exposure to the static magnetic fields of NMR can affect apoptosis induced on reporter tumor cells of haematopoietic origin. The impressive result was the strong increase (1.8–2.5 fold) of damage-induced apoptosis by NMR. This potentiation is due to cytosolic Ca2+ overload consequent to NMR-promoted Ca2+ influx, since it is prevented by intracellular (BAPTA-AM) and extracellular (EGTA) Ca2+ chelation or by inhibition of plasma membrane L-type Ca2+ channels. Three-days follow up of treated cultures shows that NMR decrease long term cell survival, thus increasing the efficiency of cytocidal treatments. Importantly, mononuclear white blood cells are not sensitised to apoptosis by NMR, showing that NMR may increase the differential cytotoxicity of antitumor drugs on tumor vs normal cells. This strong, differential potentiating effect of NMR on tumor cell apoptosis may have important implications, being in fact a possible adjuvant for antitumor therapies.
Keywordsapoptosis blood cells Ca2+ influx etoposide NMR static magnetic field tumor cells
Unable to display preview. Download preview PDF.
- 3.Cantoni O, Sestili P, Fiorani M, Dacha M. Effect of 50 Hz sinusoidal electric and/or magnetic fields on the rate of repair of DNA single strand breacks in cultured mammalian cells exposed to three different carginogens: methylmetha ne sulphonate, chromate and 254 nm UV radiation. Biochem Mol Biol Int 1996; 38: 527–533.PubMedGoogle Scholar
- 12.Liboff AR. Cyclotron resonance in membrane transport. In A. Chiabrera et al., eds. Interactions between electromagnetic fields and cells. Plenum, New York, 1985: 281–296.Google Scholar
- 24.Ding GR, Nakahara T, Tian FR, Guo Y, Miyakoshi J. Transient suppression of X-ray-induced apoptosis by exposure to power frequency magnetic fields in MCF-7 cells.Biochem Biophys Res Commun 2001; 286: 953–957.Google Scholar