Apoptosis

, Volume 11, Issue 11, pp 1969–1975 | Cite as

P38 MAPK protects against TNF-α-provoked apoptosis in LNCaP prostatic cancer cells

  • M. Ricote
  • I. García-Tuñón
  • B. Fraile
  • C. Fernández
  • P. Aller
  • R. Paniagua
  • M. Royuela
Article

Abstract

Purpose: One of the most relevant aspects in cell death regulation is the signalling of apoptosis by the serine/threonine kinases MAPKs. The aim of this study was to investigate the effects of TNF-α stimulation on MAPK activation, and the pro- or anti-apoptotic role of these kinases in LNCaP and PC3 cells. Material and methods: Treatments were carried out using several TNF-α concentrations, as well as specific pharmacological inhibitors of MAPKs. Apoptosis rates were evaluated by DAPI staining and flow cytometry. MAPK phosphorylation/activation was measured by Western blot. Results: TNF-α induced apoptosis in a dose-dependent manner in LNCaP but not in PC3 cells. The MAPK inhibitors revealed that the apoptotic rate in LNCaP cells increased significantly following p38 inhibition. The kinase inhibitors failed to cause changes in apoptosis in PC3 cells. Conclusions: The potentiation of apoptosis by p38 inhibition points to this kinase as a possible target for the treatment of androgen-dependent prostatic cancer.

Keywords

Apoptosis Protate Cancer LNCap PC3 P38 TNF 

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Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • M. Ricote
    • 1
  • I. García-Tuñón
    • 1
  • B. Fraile
    • 1
  • C. Fernández
    • 2
  • P. Aller
    • 2
  • R. Paniagua
    • 1
  • M. Royuela
    • 1
  1. 1.Departmento de Biología Celular y GenéticaUniversidad de AlcaláMadridSpain
  2. 2.Centro de Investigaciones BiológicasConsejo Superior de Investigaciones CientíficasMadridSpain

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