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Apoptosis

, Volume 10, Issue 1, pp 167–176 | Cite as

Recruitment of TRADD, FADD, and caspase 8 to double-stranded RNA-triggered death inducing signaling complexes (dsRNA-DISCs)

  • M. S. Iordanov
  • J. D. Kirsch
  • O. P. Ryabinina
  • J. Wong
  • P. N. Spitz
  • V. B. Korcheva
  • A. Thorburn
  • B. E. MagunEmail author
Article

Abstract

Rapid elimination of virus-infected cells by apoptosis is an efficient anti-viral strategy. Double-stranded RNA (dsRNA), a viral product, is potently and rapidly apoptogenic in susceptible cells. Caspase 8 plays an important role in the dsRNA-induced apoptosis; however, the mechanisms of caspase 8 activation in response to dsRNA are unknown. We demonstrate here that, in HeLa cells, the dsRNA-triggered activation of caspase 8 is independent of ongoing proteins synthesis (and is, therefore, independent of changes in pro- and anti-apoptotic gene expression) and involves the formation of multiprotein dsRNA-triggered death inducing signaling complexes (dsRNA-DISCs). DsRNA-DISCs contain FADD, TRADD, and caspase 8; however, several experimental approaches suggest that death ligands and death receptors (such as Fas/Apo1 and DR4/Apo2) are not involved in the formation of dsRNA-DISCs.

Keywords:

apoptosis caspases death receptor DISC double-stranded FADD TRADD 

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • M. S. Iordanov
    • 1
  • J. D. Kirsch
    • 1
  • O. P. Ryabinina
    • 1
  • J. Wong
    • 1
  • P. N. Spitz
    • 1
  • V. B. Korcheva
    • 1
  • A. Thorburn
    • 2
  • B. E. Magun
    • 1
    Email author
  1. 1.Department of Cell and Developmental BiologyOregon Health & Science UniversityPortland
  2. 2.Department of Cancer BiologyWake Forest University School of MedicineWinston-Salem

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