Experimental and Applied Acarology

, Volume 80, Issue 1, pp 59–70 | Cite as

Methods for extraction and ex-vivo experimentation with the most complex human commensal, Demodex spp.

  • Benjamin M. Clanner-Engelshofen
  • Lars E. French
  • Markus ReinholzEmail author


Demodex spp. mites are the most complex organisms of the human skin microbiome and were discovered more than 175 years ago, yet only little basic research is published about them. As they can be pathophysiologically relevant ectoparasites associated with rosacea, pityriasis folliculorum, and other inflammatory skin diseases, more research should be encouraged. Being a large microorganism or a tiny animal, there are no established basic methods to handle these mites. Here, we describe techniques enabling the extraction of Demodex mites from human skin, their analysis in different ex-vivo settings, the lysis of their exoskeleton, their preservation by freezing, and observation microscopically using specific fluorescent dyes or their inherent autofluorescence. These procedures should facilitate future Demodex research and fuel further the generation of knowledge. Furthermore it is intended to ultimatively enable the mite’s cultivation in vitro and reveal its pathophysiological mechanisms.


Demodicidae Mite Fluorescence microscopy Viability DNA extraction 



  1. Aytekin S, Yaşar Ş, Göktay F, Güneş P (2016) Spontaneous fluorescence of Demodex in the dark. J Eur Acad Dermatol Venereol 30(2):359–360. DOI: CrossRefPubMedGoogle Scholar
  2. Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77(1):71–94PubMedPubMedCentralGoogle Scholar
  3. Cardwell LA, Alinia H, Moradi Tuchayi S, Feldman SR (2016) New developments in the treatment of rosacea-role of once-daily ivermectin cream. Clin Cosmet Investig Dermatol 9:71–77. CrossRefPubMedPubMedCentralGoogle Scholar
  4. Chen W, Plewig G (2014) Human demodicosis: revisit and a proposed classification. Br J Dermatol 170(6):1219–1225. DOI: CrossRefPubMedGoogle Scholar
  5. Chen WenChieh, Plewig G (2015) Are Demodex mites principal, conspirator, accomplice, witness or bystander in the cause of rosacea? Am J Clin Dermatol 16(2):67–72. CrossRefPubMedGoogle Scholar
  6. Clanner-Engelshofen BM, Ruzicka T, Reinholz M (2018) Efficient isolation and observation of the most complex human commensal, Demodex spp. Exp Appl Acarol 76(1):71–80. CrossRefPubMedGoogle Scholar
  7. Clanner-Engelshofen BM, French LE, Reinholz M (2019) Corynebacterium kroppenstedtii subsp. demodicis is the endobacterium of Demodex folliculorum. J Eur Acad Dermatol Venereol. DOI: [epub ahead of print ).CrossRefPubMedGoogle Scholar
  8. Desch C (1989) The digestive system of Demodex folliculorum (Acari: Democidae) of man: a light and electron microscope study. Prog Acarol 1:187–195Google Scholar
  9. Gatault S, Foley R, Shiels L, Powell FC (2019) Evaluation of Demodex mite viability using motility and scattered light intensity. Exp Appl Acarol 77(4):463–469. DOI: CrossRefPubMedGoogle Scholar
  10. Lacey N, Russell-Hallinan A, Powell FC (2016) Study of Demodex mites: challenges and solutions. J Eur Acad Dermatol Venereol 30(5):764–775. CrossRefPubMedGoogle Scholar
  11. Lacey N, Russell-Hallinan A, Zouboulis CC, Powell FC (2018) Demodex mites modulate sebocyte immune reaction: possible role in the pathogenesis of rosacea. Br J Dermatol. CrossRefPubMedGoogle Scholar
  12. Marks R, Dawber RP (1971) Skin surface biopsy. An improved technique for the examination of the horny layer. Br J Dermatol 84(2):117–123CrossRefGoogle Scholar
  13. Pillai CKS, Paul W, Sharma CP (2009) Chitin and chitosan polymers: chemistry, solubility and fiber formation. Prog Polym Sci 34(7):641–678. CrossRefGoogle Scholar
  14. Spickett SG (1961) Studies on Demodex folliculorum Simon (1842). I. Life history. Parasitology 51(1–2):181–192. CrossRefGoogle Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Dermatology and Allergy, University HospitalLudwig-Maximilian-University MunichMunichGermany

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