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Experimental and Applied Acarology

, Volume 77, Issue 2, pp 215–228 | Cite as

A high gene flow in populations of Amblyomma ovale ticks found in distinct fragments of Brazilian Atlantic rainforest

  • Gislene F. S. R. Fournier
  • Adriano Pinter
  • Renata Santiago
  • Sebastian Muñoz-Leal
  • Thiago Fernandes Martins
  • Marcos Gomes Lopes
  • Karen D. McCoy
  • Celine Toty
  • Mauricio C. Horta
  • Marcelo B. Labruna
  • Ricardo A. DiasEmail author
Article

Abstract

The genetic structure of populations of the tick Amblyomma ovale from five distinct areas of the Brazilian Atlantic rainforest was evaluated via DNA sequencing and associated with the presence of domestic dogs acting as hosts at the edge of forest fragments. Ticks were collected from domestic dogs and from the environment between 2015 and 2017. Four collection areas were located in the surroundings and within the Serra do Mar State Park, São Paulo State (23°37′21"S, 45°24′43"W), where dogs were bimonthly monitored along 2 years using camera traps and GSM trackers. To determine the spatial limits of genetic structure, ticks collected upon dogs living near the Serra do Baturié, Ceará State (4°15′40"S, 38°55′54"W) were included as well. A total of 39 haplotypes of 16S rRNA and Cox 1 mitochondrial genes sequences were observed, with 27 of them coming from areas within the Serra do Mar State Park. No haplotype was shared between the Serra do Mar and the Serra do Baturié indicating isolation of tick populations at the scale of 2000 km. Although three different haplotype lineages of A. ovale occurred within the Serra do Mar State Park, no genetic structure was found across the study sites within this park, suggesting high tick gene flow across a range of 45 km. Monitoring data from domestic dogs and wild carnivores showed that these species share the same habitats at the forest edge, with dogs playing a likely limited role in tick dispersal. Our findings have important implications for understanding the genetic structure of wide spread A. ovale along Brazilian rainforest remnants, which can further be associated to tick-borne infectious agents, such as Rickettsia parkeri, and used for predicting future patterns of tick diversity in the Brazilian Atlantic rainforest.

Keywords

Amblyomma ovale Tick Gene flow Brazil Rainforest 

Notes

Acknowledgements

We would like to thank Olivier Duron for helpful discussions. This work was not possible without financial support from FAPESP from processes 2014/648-3 and 2016/5355-0 (BEPE). Partial funding for this work came from the ANR Grant ESPEVEC (ANR blanc ANR-13-BSV7-0018-01) to KDM. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brasil (CAPES)–Finance Code 001.

Supplementary material

10493_2019_350_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 12 KB)
10493_2019_350_MOESM2_ESM.tif (12.7 mb)
Figure 3 Gray-scale heatmap matrixes calculated upon the alignments of 275 bp partial fragments of 16S rRNA (A) and cox1 (B) genes from Serra do Baturié (A5) and Serra do Mar State Park (A1–A4) populations of A. ovale. In each tree, Serra do Baturié sequences are denoted with orange and Serra do Mar State Park sequences with blue branches. Evolutionary relationships were inferred using Tamura-Nei genetic distance model. Support values obtained after 1000 bootstrap replications are indicated for main branches. Resulting trees were midpoint-rooted and transformed to cladrograms (TIF 12968 KB)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Gislene F. S. R. Fournier
    • 1
  • Adriano Pinter
    • 2
  • Renata Santiago
    • 3
  • Sebastian Muñoz-Leal
    • 1
  • Thiago Fernandes Martins
    • 1
  • Marcos Gomes Lopes
    • 1
  • Karen D. McCoy
    • 4
  • Celine Toty
    • 4
  • Mauricio C. Horta
    • 5
  • Marcelo B. Labruna
    • 1
  • Ricardo A. Dias
    • 1
    Email author
  1. 1.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária eZootecnia Universidade de São PauloSão PauloBrazil
  2. 2.Superintendência de Controle de Endemias de São PauloSão PauloBrazil
  3. 3.Departamento de Biologia Geral, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  4. 4.MIVEGEC UMR 5290 CNRS-IRD-Université de Montpellier, Centre IRDMontpellierFrance
  5. 5.Universidade Federal do Vale do São FranciscoPetrolinaBrazil

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