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Characterization of the bacterial community in Haemaphysalis longicornis (Acari: Ixodidae) throughout developmental stages

  • Zhang RuilingEmail author
  • Huang Zhendong
  • Yu Guangfu
  • Zhang ZhongEmail author
Article

Abstract

As one of the most important vectors, Haemaphysalis longicornis can transmit a variety of pathogens and is widely distributed in China. It has been reported that the bacterial community in ticks can impact tick fitness, development, and reproduction and even the transmission of tick-borne pathogens. In this study, bacterial diversity across all developmental stages (eggs, larvae, nymphs and adults) of H. longicornis was investigated using high-throughput sequencing technology. The results demonstrated that Proteobacteria was the dominant phylum and that Coxiella was the most abundant bacterial genus across all the samples. Alpha diversity analysis demonstrated that the eggs had the highest bacterial richness and diversity, and the bacterial community of the larvae was found to be similar to that of the eggs. However, there was a rapid increase in the relative abundance of Coxiella upon development of larvae to nymphs. Females exhibited the lowest bacterial diversity, and the proportion of Coxiella decreased from 85% in females to 45% in males. Our results suggest that H. longicornis lost most of the bacteria present in the early developmental stages and re-established the bacterial community after bloodmeals and molting.

Keywords

16S rRNA sequencing Proteobacteria Coxiella Tick-borne disease 

Notes

Acknowlegements

This research was supported by development plan project of Shandong province science and technology (No. 2017GSF221017) and National Natural Sciences Foundation of China (No. 81,871,686).

Supplementary material

10493_2019_339_MOESM1_ESM.tif (6 mb)
Rarefaction curves of all samples used in this study (TIF 6096 KB)
10493_2019_339_MOESM2_ESM.tif (23.9 mb)
Most abundant bacterial genus detected in different samples (TIF 24450 KB)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Collaborative Innovation Center for the Origin and Control of Emerging Infectious DiseasesTaishan Medical UniversityTaianChina
  2. 2.School of Basic Medical ScienceTaishan Medical UniversityTaianChina

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