An Ixodes scapularis cell line with a predominantly neuron-like phenotype
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The Ixodes scapularis embryo-derived cell line ISE6 is the most widely utilized tick-derived cell line due to its susceptibility to a wide variety of tick- and non-tick-vectored pathogens. Little is known about its tissue origin or biological background. Protein expression of ISE6 cells was compared with that of another I. scapularis-derived cell line, IDE12, and dissected tick synganglia. Results demonstrated the presence of a neuronal marker protein, type 3 β-tubulin, in all three samples, as well as other shared and unique neuronal and immune response-associated proteins. Of neuronal proteins shared between the two cell lines, ISE6 expressed several in significantly greater quantities than IDE12. Stimulation of ISE6 cells by in vivo exposure to the hemocoel environment in unfed larval and molting nymphal ticks, but not unfed nymphal ticks, resulted in the development of neuron-like morphologic characteristics in the implanted cells.
KeywordsIxodes scapularis Cell culture ISE6 IDE12 Proteome Synganglion Neuron
This research was funded by grants from the US National Institutes of Health, Numbers R01AI042792 and R01AI049424 to Ulrike G. Munderloh. Mass spectrometry was performed at The Center for Mass Spectrometry and Proteomics at the University of Minnesota.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the University of Minnesota. Hamsters were maintained in accordance with an approved University of Minnesota IACUC protocol.
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