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Experimental and Applied Acarology

, Volume 66, Issue 2, pp 247–256 | Cite as

Variation among Bm86 sequences in Rhipicephalus (Boophilus) microplus ticks collected from cattle across Thailand

  • S. Kaewmongkol
  • G. Kaewmongkol
  • N. Inthong
  • N. Lakkitjaroen
  • T. Sirinarumitr
  • C. M. Berry
  • N. N. Jonsson
  • R. W. Stich
  • S. Jittapalapong
Article

Abstract

Anti-tick vaccines based on recombinant homologues Bm86 and Bm95 have become a more cost-effective and sustainable alternative to chemical pesticides commonly used to control the cattle tick, Rhipicephalus (Boophilus) microplus. However, Bm86 polymorphism among geographically separate ticks is reportedly associated with reduced effectiveness of these vaccines. The purpose of this study was to investigate the variation of Bm86 among cattle ticks collected from Northern, Northeastern, Central and Southern areas across Thailand. Bm86 cDNA and deduced amino acid sequences representing 29 female tick midgut samples were 95.6–97.0 and 91.5–93.5 % identical to the nucleotide and amino acid reference sequences, respectively, of the Australian Yeerongpilly vaccine strain. Multiple sequence analyses of these Bm86 variants indicated geographical relationships and polymorphism among Thai cattle ticks. Two larger groups of cattle tick strains were discernable based on this phylogenetic analysis of Bm86, a Thai group and a Latin American group. Thai female and male cattle ticks (50 pairs) were also subjected to detailed morphological characterization to confirm their identity. The majority of female ticks had morphological features consistent with those described for R. (B.) microplus, whereas, curiously, the majority of male ticks were more consistent with the recently re-instated R. (B.) australis. A number of these ticks had features consistent with both species. Further investigations are warranted to test the efficacies of rBm86-based vaccines to homologous and heterologous challenge infestations with Thai tick strains and for in-depth study of the phylogeny of Thai cattle ticks.

Keywords

Ticks Rhipicephalus (Boophilus) microplus Bm86 Phylogenetic analysis 

Notes

Acknowledgments

This study was granted by Kasetsart University Research and Development Institute (KURDI) and Center for Agricultural Biotechnology (CAB), Kasetsart University, Kamphaeng Sean Campus and Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Commission on Higher Education, Ministry of Education. (AG-BIO/PERDO-CHE). This work was also supported by the Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University Under the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, Ministry of Education, Thailand.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • S. Kaewmongkol
    • 1
    • 2
    • 3
    • 4
  • G. Kaewmongkol
    • 5
  • N. Inthong
    • 4
  • N. Lakkitjaroen
    • 4
  • T. Sirinarumitr
    • 6
  • C. M. Berry
    • 7
  • N. N. Jonsson
    • 7
  • R. W. Stich
    • 8
  • S. Jittapalapong
    • 9
  1. 1.Center for Agricultural BiotechnologyKasetsart UniversityNakhon PathomThailand
  2. 2.Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE)BangkokThailand
  3. 3.Center for Advanced Studies for Agricultural and Food, KU Institute for Advanced StudiesKasetsart University (CASAF, NRU-KU, Thailand)BangkokThailand
  4. 4.Department of Veterinary Technology, Faculty of Veterinary TechnologyKasetsart UniversityBangkokThailand
  5. 5.Department of Companion Animal Clinical Sciences, Faculty of Veterinary MedicineKasetsart UniversityBangkokThailand
  6. 6.Department of Pathology, Faculty of Veterinary MedicineKasetsart UniversityBangkokThailand
  7. 7.Institute of Biodiversity, Animal Health and Comparative MedicineUniversity of GlasgowGlasgowUK
  8. 8.Department of Veterinary PathobiologyUniversity of MissouriColumbiaUSA
  9. 9.Department of Parasitology, Faculty of Veterinary MedicineKasetsart UniversityBangkokThailand

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