Experimental and Applied Acarology

, Volume 63, Issue 1, pp 77–83 | Cite as

Influence of tea tree oil (Melaleuca alternifolia) on the cattle tick Rhipicephalusmicroplus

  • Rafael Pazinato
  • Vanderlei Klauck
  • Andreia Volpato
  • Alexandre A. Tonin
  • Roberto C. Santos
  • Márcia E. de Souza
  • Rodrigo A. Vaucher
  • Renata Raffin
  • Patrícia Gomes
  • Candice C. Felippi
  • Lenita M. Stefani
  • Aleksandro S. Da Silva
Article

Abstract

The aim of this study was to verify the influence of tea tree oil (TTO) (Melaleuca alternifolia) tested in its pure and nanostructured (TTO nanoparticles) forms on the reproduction of female Rhipicephalusmicroplus. For our purpose, female ticks were collected from naturally infected animals and treated in vitro with TTO (1, 5, and 10 %) and TTO nanoparticles (0.075, 0.375, and 0.75 %). In order to validate the tests, they were performed in triplicate using positive (amitraz) and negative (untreated) controls. It was possible to observe that pure TTO (5 and 10 %) and TTO nanoparticles (0.375 and 0.75 %) showed 100 % reproductive inhibition on female ticks. Additionally, pure TTO (1 %) also showed an acaricide effect (70 %), similarly to the positive control (78.3 %). This is the first study demonstrating the activity of pure TTO and TTO nanoparticles on female ticks. Therefore, based on these results, we were able to show that both forms and all concentrations of M. alternifolia affected tick reproduction by inhibiting egg laying and hatching. We were also able to show that TTO nanoparticles potentiated the inhibitor effect of pure TTO on the reproduction of R. microplus.

Keywords

Ticks Natural products Tea tree oil Nanotechnology 

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Rafael Pazinato
    • 1
  • Vanderlei Klauck
    • 1
  • Andreia Volpato
    • 1
  • Alexandre A. Tonin
    • 2
  • Roberto C. Santos
    • 3
    • 4
  • Márcia E. de Souza
    • 3
    • 4
  • Rodrigo A. Vaucher
    • 3
  • Renata Raffin
    • 4
  • Patrícia Gomes
    • 4
  • Candice C. Felippi
    • 5
  • Lenita M. Stefani
    • 6
  • Aleksandro S. Da Silva
    • 1
  1. 1.Department of Animal ScienceUniversidade do Estado de Santa CatarinaChapecóBrazil
  2. 2.Department of Microbiology and ParasitologyUniversidade Federal de Santa MariaSanta MariaBrazil
  3. 3.Laboratory of Microbiology, Center of Health ScienceCentro Universitário Franciscano-UNIFRASanta MariaBrazil
  4. 4.Laboratory of Nanotechnology, Programa de Pós-Graduação em NanociênciasUNIFRASanta MariaBrazil
  5. 5.InventivaPorto AlegreBrazil
  6. 6.Graduate School of Animal ScienceUniversidade do Estado de Santa CatarinaLagesBrazil

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