Experimental and Applied Acarology

, Volume 60, Issue 4, pp 521–530 | Cite as

Spread and strain determination of Varroa destructor (Acari: Varroidae) in Madagascar since its first report in 2010

  • Henriette Rasolofoarivao
  • Johanna Clémencet
  • Lala Harivelo Raveloson Ravaomanarivo
  • Dimby Razafindrazaka
  • Bernard Reynaud
  • Hélène DelatteEmail author


Varroa destructor is a major pest in world beekeeping. It was first detected in Madagascar in 2010 on the endemic honeybee Apis mellifera unicolor. To evaluate V. destructor spread dynamics in Madagascar a global survey was conducted in 2011–2012. A total of 695 colonies from 30 districts were inspected for the presence of mites. 2 years after its introduction, nine districts were found infested. Varroa destructor spread was relatively slow compared to other countries with a maximum progression of 40 km per year, the five newly infested districts being located next to the first infested ones. The incidence of mite infestation was also investigated by monitoring 73 colonies from five apiaries during 1 year (2011–2012). Sixty percent of local colony mortality was recorded after 1 year of survey. Varroa destructor strain determination was done by partial sequencing of the cytochrome oxidase I gene of 13 phoretic mites sampled in five districts. A single V. destructor mitochondrial haplotype was detected, the Korean type, also present in the closest African countries. A global pathogen survey was also conducted on the colonies inspected for mite presence. The greater wax moth, Galleria mellonella has been found in all colonies all over the country. Two other pathogens and morphological abnormalities in workers, such as deformed wings, were found associated with only V. destructor presence. A prevention management plan must be implemented to delimit mite spread across the island.


Varroa destructor Apis mellifera unicolor Madagascar Mitochondrial DNA 



We thank Patrick Turpin for mapping sites with GIS and Christoph Simiand for technical help in the laboratory. We thank the following people for their help with data collection: Vincent Porphyre, Carlos Borsa, Valentina Mandirola, Niaina Andrianaivoariseta, Julien Cattel, Quentin Chesnais and ADEFA. We are grateful to the malagasy beekeepers who participated in the study. This work is part of the PhD of Henriette Rasolofoarivao recipient of a grant of CIRAD-AIRD-Sud. Field work had been partly funded by CIRAD, the Enlargement and sustainability of the Plant Protection Network (ePRPV) project supported by the European Union, the French government, and Réunion collectivities.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Henriette Rasolofoarivao
    • 1
    • 2
  • Johanna Clémencet
    • 3
  • Lala Harivelo Raveloson Ravaomanarivo
    • 2
  • Dimby Razafindrazaka
    • 2
  • Bernard Reynaud
    • 1
  • Hélène Delatte
    • 1
    Email author
  1. 1.CIRAD, UMR PVBMTSaint-Pierre, La RéunionFrance
  2. 2.Département d’Entomologie, Faculté de SciencesUniversité d’AntananarivoAntananarivoMadagascar
  3. 3.Université de La Réunion, UMR PVBMTSaint Denis cedex 9, La RéunionFrance

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