Advertisement

Experimental and Applied Acarology

, Volume 56, Issue 2, pp 143–150 | Cite as

Life cycle of Haemaphysalis doenitzi (Acari: Ixodidae) under laboratory conditions and its phylogeny based on mitochondrial 16S rDNA

  • Xuejie Chen
  • Zhijun Yu
  • Lida Guo
  • Lingxia Li
  • Hao Meng
  • Duo Wang
  • Renjie Liu
  • Jingze Liu
Article

Abstract

The paper investigated the life cycle of the hard tick Haemaphysalis doenitzi under laboratory conditions and its phylogeny based on mitochondrial 16S rDNA. The results revealed that the complete life cycle of H. doenitzi requires a mean duration of 109.6 days ranging from 91 to 137 days and the average prefeeding, feeding and premoulting periods of larvae, nymphs and females and the eggs hatching period are 18.7, 26.9, 38.9, and 25.1 days, respectively. In addition, the weight of engorged females is highly correlated with the number of egg masses laid (r = 0.936, P < 0.001). The female reproductive efficiency index and reproductive fitness index are 13.4 and 12.8, respectively. The mean weight of the engorged nymphs (2.77 mg) moulting to females is much higher than those (1.68 mg) moulting to males, which could be used as an index to predict sexes in this species. The ratio of male to female is 1:1.01. Moreover, multisequence alignments and phylogenetic tree constructed based on the mitochondrial 16S rDNA sequences suggest that H. doenitzi is genetically close to H. longicornis.

Keywords

Haemaphysalis doenitzi Ixodid tick Life cycle 16S rDNA Phylogeny 

Notes

Acknowledgments

This project was supported by the National Natural Science Foundation of China (30970406) and the Key Basic Research Project of Hebei Province (11965516D). The authors are very grateful to Jiagang Sun (Director of Animal Husbandry and Food Bureau of Cangxi County, Sichuan, China) for the help in tick collection.

References

  1. Ahantarig A, Trinachartvanit W, Milne JR (2008) Tick-borne pathogens and diseases of animals and humans in Thailand. Southeast Asian J Trop Med Public Health 39:1015–1032PubMedGoogle Scholar
  2. Ahmed BM, Taha KM EI, Hussein AM (2011) Life cycle of Hyalomma anatolicum Koch, 1844 (Acari: Ixodidae) fed on rabbits, sheep and goats. Vet Parasitol 177:353–358PubMedCrossRefGoogle Scholar
  3. Barker SC, Murrell A (2004) Systematics and evolution of ticks with a list of valid genus and species names. Parasitology 129:15–36CrossRefGoogle Scholar
  4. Chen Z, Yu Z, Yang X, Zheng H, Liu J (2009) The life cycle of Hyalomma asiaticum kozlovi Olenev, 1931 (Acari: Ixodidae) under laboratory conditions. Vet Parasitol 160:134–137PubMedCrossRefGoogle Scholar
  5. Chen Z, Yang X, Bu F, Yang X, Yang X, Liu J (2010) Ticks (Acari: Ixodoidea: Argasidae, Ixodidae) of China. Exp Appl Acarol 51:393–404PubMedCrossRefGoogle Scholar
  6. Chilton NB (1992) An index to assess the reproductive fitness of female ticks. Int J Parasitol 22:109–111PubMedCrossRefGoogle Scholar
  7. Dantrakool A, Somboon P, Hashimoto T, Saito-Ito A (2004) Identification of a new type of Babesia species in wild rats (Bandicota indica) in Chiang Mai Province, Thailand. J Clin Microbiol 42:850–854PubMedCrossRefGoogle Scholar
  8. Despins JL (1992) Effects of temperature and humidity on ovipositional biology and egg development of the tropical horse tick, Dermacentor (Anocentor) nitens. J Med Entomol 29:332–337PubMedGoogle Scholar
  9. Doube BM, Kemp DH (1979) Notes on the tick Haemaphysalis (Ornithophysalis) doenitzi Warburton and Nuttall (Acarina: Ixodidae) in Australia. Aust J Entomol 18:169–170CrossRefGoogle Scholar
  10. Gladney WJ, Dawkins CC, Price MA (1977) Amblyomma inornatum (Acarina: Ixodidae): natural hosts and laboratory biology. J Med Entomol 14:85–88PubMedGoogle Scholar
  11. Hoogstraal H, Wassef HY (1973) The Haemaphysalis ticks (Ixodoidea: Ixodidae) of birds. 3. H. (Ornithophysalis) subgen. n.: definition, species, hosts, and distribution in the Oriental, Palearctic, Malagasy, and Ethiopian faunal regions. J Parasitol 59:1099–1117PubMedCrossRefGoogle Scholar
  12. Hu R, Rowley WA (2000) Relationship between weights of the engorged nymphal stage and resultant sexes in Ixodes scapularis and Dermacentor variabilis (Acari: Ixodidae) ticks. J Med Entomol 37:198–200PubMedCrossRefGoogle Scholar
  13. Jongejan F, Uilenberg G (2004) The global importance of ticks. Parasitology 129:3–14CrossRefGoogle Scholar
  14. Labruna MB, Kasai N, Ferreira F, Faccini JLH, Gennari SM (2002) Seasonal dynamics of ticks (Acari: Ixodidae) on horses in the state of São Paulo, Brazil. Vet Parasitol 105:65–77PubMedCrossRefGoogle Scholar
  15. Labruna MB, Pinter A, Teixeira RHF (2004) Life cycle of Amblyomma cooperi (Acari: Ixodidae) using capybaras (Hydrochaeris hydrochaeris) as hosts. Exp Appl Acarol 32:79–88PubMedCrossRefGoogle Scholar
  16. Liu J, Liu Z, Zhang Y, Yang X, Gao Z (2005) Biology of Dermacentor silvarum (Acari: Ixodidae) under laboratory conditions. Exp Appl Acarol 36:131–138PubMedCrossRefGoogle Scholar
  17. Perret JL, Rais O, Gern L (2004) Influence of climate on the proportion of Ixodes ricinus nymphs and adults questing in a tick population. J Med Entomol 41:361–365PubMedCrossRefGoogle Scholar
  18. Robbins RG (2005) The ticks (Acari: Ixodida: Argasidae, Ixodidae) of Taiwan: a synonymic checklist. Proc Entomol Soc Wash 107:245–253Google Scholar
  19. Teng KF, Jiang ZJ (eds) (1991) Economic Insect Fauna of China. Fasc 39 Acari: Ixodidae. Science Press, Beijing (in Chinese)Google Scholar
  20. Tian Z, Liu G, Xie J, Yin H, Luo J, Zhang L, Zhang P, Luo J (2011) Discrimination between Haemaphysalis longicornis and H. qinghaiensis based on the partial 16S rDNA and the second internal transcribed spacer (ITS-2). Exp Appl Acarol 54:165–172PubMedCrossRefGoogle Scholar
  21. Troughton DR, Levin ML (2007) Life cycles of seven ixodid tick species (Acari: Ixodidae) under standardized laboratory conditions. J Med Entomol 44:732–740PubMedCrossRefGoogle Scholar
  22. Yano Y, Shiraishi S, Uchida TA (1987) Effects of temperature on development and growth in the tick, Haemaphysalis longicornis. Exp Appl Acarol 3:73–78PubMedCrossRefGoogle Scholar
  23. Yeruham I, Hadani A, Galker F (2000) The life cycle of Rhipicephalus bursa Canestrini and Fanzago, 1877 (Acarina: Ixodidae) under laboratory conditions. Vet Parasitol 89:109–116PubMedCrossRefGoogle Scholar
  24. Yu Z, Zheng H, Chen Z, Zheng B, Ma H, Liu J (2010) The life cycle and biological characteristics of Dermacentor silvarum Olenev (Acari: Ixodidae) under field conditions. Vet Parasitol 168:323–328PubMedCrossRefGoogle Scholar
  25. Zhang J, Chen Z, Liu J, Yang X (2006) Study on oviposition and egg development of three species of hard ticks (Acari: Ixodidae) under laboratory conditions. Chin J Pest Control 22:864–867 (in Chinese with English abstract)Google Scholar
  26. Zheng H, Yu Z, Chen Z, Zhou L, Zheng B, Ma H, Liu J (2011) Development and biological characteristics of Haemaphysalis longicornis (Acari: Ixodidae) under field conditions. Exp Appl Acarol 53:377–388PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Xuejie Chen
    • 1
  • Zhijun Yu
    • 1
  • Lida Guo
    • 1
    • 2
  • Lingxia Li
    • 1
  • Hao Meng
    • 1
  • Duo Wang
    • 1
  • Renjie Liu
    • 1
  • Jingze Liu
    • 1
  1. 1.Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei ProvinceCollege of Life Sciences, Hebei Normal UniversityShijiazhuangChina
  2. 2.Department of Environment and Chemical EngineeringHebei College of Industry and TechnologyShijiazhuangChina

Personalised recommendations