Life cycle of Haemaphysalis doenitzi (Acari: Ixodidae) under laboratory conditions and its phylogeny based on mitochondrial 16S rDNA
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The paper investigated the life cycle of the hard tick Haemaphysalis doenitzi under laboratory conditions and its phylogeny based on mitochondrial 16S rDNA. The results revealed that the complete life cycle of H. doenitzi requires a mean duration of 109.6 days ranging from 91 to 137 days and the average prefeeding, feeding and premoulting periods of larvae, nymphs and females and the eggs hatching period are 18.7, 26.9, 38.9, and 25.1 days, respectively. In addition, the weight of engorged females is highly correlated with the number of egg masses laid (r = 0.936, P < 0.001). The female reproductive efficiency index and reproductive fitness index are 13.4 and 12.8, respectively. The mean weight of the engorged nymphs (2.77 mg) moulting to females is much higher than those (1.68 mg) moulting to males, which could be used as an index to predict sexes in this species. The ratio of male to female is 1:1.01. Moreover, multisequence alignments and phylogenetic tree constructed based on the mitochondrial 16S rDNA sequences suggest that H. doenitzi is genetically close to H. longicornis.
KeywordsHaemaphysalis doenitzi Ixodid tick Life cycle 16S rDNA Phylogeny
This project was supported by the National Natural Science Foundation of China (30970406) and the Key Basic Research Project of Hebei Province (11965516D). The authors are very grateful to Jiagang Sun (Director of Animal Husbandry and Food Bureau of Cangxi County, Sichuan, China) for the help in tick collection.
- Robbins RG (2005) The ticks (Acari: Ixodida: Argasidae, Ixodidae) of Taiwan: a synonymic checklist. Proc Entomol Soc Wash 107:245–253Google Scholar
- Teng KF, Jiang ZJ (eds) (1991) Economic Insect Fauna of China. Fasc 39 Acari: Ixodidae. Science Press, Beijing (in Chinese)Google Scholar
- Zhang J, Chen Z, Liu J, Yang X (2006) Study on oviposition and egg development of three species of hard ticks (Acari: Ixodidae) under laboratory conditions. Chin J Pest Control 22:864–867 (in Chinese with English abstract)Google Scholar