Experimental and Applied Acarology

, Volume 53, Issue 2, pp 167–178 | Cite as

Female ixodid ticks grow endocuticle during the rapid phase of engorgement

  • Peter C. Flynn
  • W. Reuben Kaufman


Lees (Proc Zool Soc Lond 121:759–772, 1952) concluded that the ixodid tick Ixodes ricinus grows endocuticle during the slow but not during the rapid, phase of engorgement, a conclusion supported by Andersen and Roepstorff (Insect Biochem Mol Biol 35:1181–1188, 2005) for the same species. In this study analysis of dimensional data and cuticle weight measurements from female ixodid ticks (Amblyomma hebraeum) were used to test this hypothesis. Both approaches showed that endocuticle growth continues during the rapid phase, tapering to zero at a fed/unfed weight ratio of ~60. Of the total mass of cuticle in the engorged tick 32–43% was formed during the rapid phase. We demonstrate that if cuticle growth stopped at the end of the slow phase, there would not be sufficient cuticle to account for the thickness of cuticle observed at the end of engorgement. This finding is consistent with prior studies of Rhipicephalus (Boophilus) microplus, and with a dimensional analysis of the cuticle thickness data of Lees for I. ricinus, in contradiction to his conclusion from an analysis of tick cuticle weight measurements. An examination of cuticle weight measurements for I. ricinus by Andersen and Roepstorff similarly supports the finding of cuticle growth during the rapid phase. All ixodid ticks undergo major body expansion, typically tenfold or more, during a rapid phase of engorgement and require sufficient cuticle at the end of that process to contain their body. The fact that cuticle grows during the rapid phase of engorgement in three species suggests that this is a general characteristic of the family Ixodidae.


Cuticle synthesis Ixodid ticks Amblyomma hebraeum Ixodes ricinus Rhipicephalus (Boophilus) microplus Rapid phase of engorgement 



This research was generously supported from a Discovery Grant to WRK from the Natural Sciences and Engineering Research Council (NSERC) of Canda. Dr. Kevin Friesen, Mr. Andrew Hulleman and Mr. Alex Smith provided some of the dimensional measurements of A. hebraeum. Professor M. Flynn of the Department of Mechanical Engineering at the University of Alberta has been a consistent source of helpful mathematical advice. Figure. 6 reproduces a Fig. 5 from Andersen and Roepstorff (2005) with the kind permission of Elsevier.


  1. Andersen SO, Roepstorff P (2005) The extensible alloscutal cuticle of the tick, Ixodes ricinus. Insect Biochem Mol Biol 35:1181–1188CrossRefPubMedGoogle Scholar
  2. Cherry LM (1973) The accumulation and utilization of food reserves by the adult female cattle tick, boophilus microplus (Canestrini). Aust J Zool 21:403–412CrossRefGoogle Scholar
  3. Diehl PA, Aeschlimann A, Obenchain FD (1982) Tick reproduction: oogenesis and oviposition. In: Obenchain FD, Galun R (eds) Physiology of ticks. Pergamon, NY, pp 277–350Google Scholar
  4. Dillinger SGC, Kessel AB (2002) Changes in the structure of the cuticle of Ixodes ricinus L. 1758 (Acari, Ixodidae) during feeding. Arthropod Struct Dev 31:95–101CrossRefPubMedGoogle Scholar
  5. Franceschini G, Bigoni D, Regitnig P, Holzapfel GA (2006) Brain tissue deforms similarly to filled elastomers and follows consolidation theory. J Mech Phys Solids 54:2592–2620CrossRefGoogle Scholar
  6. Humphrey JD (2002) Cardiovascular solid mechanics, cells tissues and organs. Springer, New YorkGoogle Scholar
  7. Kaufman WR (2007) Gluttony and sex in female ixodid ticks: how do they compare to other blood-sucking arthropods? J Insect Phys 53:264–273CrossRefGoogle Scholar
  8. Kaufman WR, Flynn PC, Reynolds SE (2010) Cuticular plasticization in the ixodid tick Amblyomma hebraeum (Acari: Ixodidae): possible roles of monamines and cuticular pH. J Exp Biol 213:2820–2831CrossRefPubMedGoogle Scholar
  9. Kitaoka S, Yajima A (1958) Physiological and ecological studies on some ticks I. Process of growth by blood-sucking. Bull Nat Inst Anim Health Tokyo 34:135–147Google Scholar
  10. Lees AD (1952) The role of cuticle growth in the feeding process of ticks. Proc Zool Soc Lond 121:750–772Google Scholar
  11. Lin HT, Dorfmann AL, Trimmer BA (2009) Soft-cuticle biomechanics: a constitutive model of anisotropy for caterpillar integument. J Theor Biol 256:447–457CrossRefPubMedGoogle Scholar
  12. Wikipedia (2010) Ellipsoid,, February 2010

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Mechanical EngineeringUniversity of AlbertaEdmontonCanada
  2. 2.Department of Biological SciencesUniversity of AlbertaEdmontonCanada

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