Predominance of Ehrlichia chaffeensis in Rhipicephalus sanguineus ticks from kennel-confined dogs in Limbe, Cameroon

  • Lucy M. Ndip
  • Roland N. Ndip
  • Seraphine N. Esemu
  • David H. Walker
  • Jere W. McBride


Rhipicephalus sanguineus ticks (n = 63) collected from five dogs (two adults and three puppies) housed in a kennel were screened for Ehrlichial agents (Ehrlichia canis, E. chaffeensis, and E. ewingii) using a species-specific multicolor real-time TaqMan PCR amplification of the disulphide bond formation protein (dsb) gene. Ehrlichia chaffeensis DNA was detected in 33 (56%) ticks, E. canis DNA was detected in four (6%) ticks, and one tick was coinfected. The E. chaffeensis and E. canis nucleotide sequences of the amplified dsb gene (374 bp) obtained from the Cameroonian R. sanguineus ticks were identical to the North American genotypes.


Rhipicephalus sanguineus Ehrlichia chaffeensis Ehrlichia canis Zoonosis Cameroon 


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Lucy M. Ndip
    • 1
  • Roland N. Ndip
    • 2
  • Seraphine N. Esemu
    • 1
  • David H. Walker
    • 3
    • 4
  • Jere W. McBride
    • 3
    • 4
    • 5
  1. 1.Department of Biochemistry and MicrobiologyUniversity of BueaBueaCameroon
  2. 2.Department of Biochemistry and MicrobiologyUniversity of Fort HareAliceSouth Africa
  3. 3.Department of PathologyUniversity of Texas Medical BranchGalvestonUSA
  4. 4.Center for Biodefense and Emerging Infectious DiseasesUniversity of Texas Medical BranchGalvestonUSA
  5. 5.Department of Microbiology and ImmunologyUniversity of Texas Medical BranchGalvestonUSA

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