Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis

  • Saiki Imamura
  • Itabajara da Silva VazJr.
  • Satoru Konnai
  • Shinji Yamada
  • Chie Nakajima
  • Misao Onuma
  • Kazuhiko Ohashi


We report the cloning, expression and characterization of an Haemaphysalis longicornis metalloprotease (named HLMP1). The gene encodes a predicted 550 aminoacid protein with similarity to metalloproteases of the reprolysin family. The protein sequence contains a signal sequence, the zinc-binding motif (HEXXHXXGXXH) common to metalloproteases and a cysteine-rich region. Reverse transcription-PCR expression analysis indicates the presence of mRNA in the salivary gland of larva, nymph and adult ticks. Rabbit repeatedly infested with H. longicornis recognized rHLMP1, suggesting that the immune-response against HLMP1 is naturally induced through the feeding of ticks. Vaccination of rabbit with rHLMP1 produced protective immunity against ticks, resulting in 15.6 and 14.6% mortality in nymph and adult ticks, respectively. This work provides information to understand the tick’s defense system, and offers new insights to develop strategies to block this defense system with an anti-tick vaccine based on a metalloprotease.


Anti-tick vaccine Metalloprotease Haemaphysalis longicornis 



This research was supported by Grant-in-Aids form the Ministry of education, Culture, Sports, Science and Technology, Government of Japan.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Saiki Imamura
    • 1
  • Itabajara da Silva VazJr.
    • 2
  • Satoru Konnai
    • 1
  • Shinji Yamada
    • 1
  • Chie Nakajima
    • 1
  • Misao Onuma
    • 1
  • Kazuhiko Ohashi
    • 1
  1. 1.Department of Disease Control, Graduate School of Veterinary MedicineHokkaido UniversitySapporoJapan
  2. 2.Centro de Biotecnologia do Estado do Rio Grande do SulUniversidade Federal do Rio Grande do SulPorto Alegre RSBrazil

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