Experimental and Applied Acarology

, Volume 44, Issue 4, pp 307–314 | Cite as

Larvae of chigger mites Neotrombicula spp. (Acari: Trombiculidae) exhibited Borrelia but no Anaplasma infections: a field study including birds from the Czech Carpathians as hosts of chiggers

  • Ivan Literak
  • Alexandr A. Stekolnikov
  • Oldrich Sychra
  • Lenka Dubska
  • Veronika Taragelova
Article

Abstract

Chigger mites were collected from 1,080 wild birds of 37 species at Certak (Czech Republic), in the western Carpathian Mountains, from 29 July to 24 September 2005. The prevalence of infestation with chigger larvae was 7%. A total of 325 chigger specimens from 10 bird species was identified and three chigger species were found: Neotrombicula autumnalis, N. carpathica, and N. inopinata, the latter two species being reported on new hosts. Neotrombicula carpathica is reported in the Czech Republic for the first time. A total of 509 chigger larvae found on 79 host specimens were examined by polymerase chain reaction (PCR) for the presence of Borrelia burgdorferi s.l. DNA (fragments of the rrf (5S)—rrl (23S) intergenic spacer), and Anaplasma phagocytophilum DNA (epank1 gene). A fragment of specific Borrelia DNA was amplified through PCR in one sample, and the PCR product was further analyzed by reverse line blotting assay, whereby both genospecies of B. garinii and B. valaisiana were proved. This sample pooled five chigger larvae collected from one Sylvia atricapilla on 11 August 2005. No A. phagocytophilum DNA was amplified. We conclude that larvae of the genus Neotrombicula can be infected with Borrelia genospecies originated from their present or former hosts.

Keywords

Neotrombicula Borrelia garinii Borrelia valaisiana Anaplasma Birds Czech Republic 

Notes

Acknowledgements

This study was funded through grant No. MSM6215712402 from the Ministry of Education, Youth and Sports of the Czech Republic and Lenka Dubska received funding through grant No. 524-08-P139 from the Czech Science Foundation. We thank Dr. Miroslav Petrovec (Department of Infectious Diseases, University of Ljubljana, Slovenia) for providing A. phagocytophilum DNA, and Dr. Elena Kocianova (Institute of Virology, Bratislava, Slovakia) for a determination of ticks Ixodes ricinus. The authors declare that the study complies with the current laws of the Czech Republic.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Ivan Literak
    • 1
  • Alexandr A. Stekolnikov
    • 2
  • Oldrich Sychra
    • 1
  • Lenka Dubska
    • 1
  • Veronika Taragelova
    • 3
  1. 1.Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and EcologyUniversity of Veterinary and Pharmaceutical SciencesBrnoCzech Republic
  2. 2.Zoological InstituteRussian Academy of SciencesSaint PetersburgRussia
  3. 3.Institute of ZoologySlovak Academy of SciencesBratislavaSlovakia

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