Experimental and Applied Acarology

, Volume 44, Issue 3, pp 227–238 | Cite as

Comparative reproduction of Varroa destructor in different types of Russian and Italian honey bee combs

  • Lilia I. de Guzman
  • Thomas E. Rinderer
  • Amanda M. Frake


Earlier studies showed that Russian honey bees support slow growth of varroa mite population. We studied whether or not comb type influenced varroa reproduction in both Russian and Italian honey bees, and whether Russian bees produced comb which inhibited varroa reproduction. The major differences found in this study concerned honey bee type. Overall, the Russian honey bees had lower (2.44 ± 0.18%) levels of varroa infestation than Italian honey bees (7.20 ± 0.60%). This decreased infestation resulted in part from a reduced number of viable female offspring per foundress in the Russian (0.85 ± 0.04 female) compared to the Italian (1.23 ± 0.04 females) honey bee colonies. In addition, there was an effect by the comb built by the Russian honey bee colonies that reduced varroa reproduction. When comparing combs having Russian or Italian colony origins, Russian honey bee colonies had more non-reproducing foundress mites and fewer viable female offspring in Russian honey bee comb. This difference did not occur in Italian colonies. The age of comb in this study had mixed effects. Older comb produced similar responses for six of the seven varroa infestation parameters measured. In colonies of Italian honey bees, the older comb (2001 dark) had fewer (1.13 ± 0.07 females) viable female offspring per foundress than were found in the 2002 new (1.21 ± 0.06 females) and 1980s new (1.36 ± 0.08 females) combs. This difference did not occur with Russian honey bee colonies where the number of viable female offspring was low in all three types of combs. This study suggests that honey bee type largely influences growth of varroa mite population in a colony.


Varroa destructor Reproduction Dark comb New comb Resistance 


  1. Baxter JR, Eischen F, Pettis J, Wilson WT, Shimanuki H (1998) Detection of fluvalinate-resistant Varroa mites in the United States. Am Bee J 138:291Google Scholar
  2. Bogdanov S, Kilchenmann V, Imdorf A (1998) Acaricide residues in some bee products. J Apic Res 37:57–67Google Scholar
  3. de Guzman LI, Rinderer TE, Frake AM (2007) Growth of Varroa destructor Anderson and Trueman (Acari: Varroidae) populations in Russian honey bee (Apis mellifera L.) (Hymenoptera: Apidae) colonies. Ann Entomol Soc Am 100:187–195CrossRefGoogle Scholar
  4. Donzé GS, Guerin PM (1994) Behavioral attributes and parental care of Varroa mites parasitizing honey bee brood. Behav Ecol Sociobiol 34:305–319Google Scholar
  5. Donzé GS, Schnyder-Candrian S, Bogdanov S, Diehl PA, Guerin PM (1998) Aliphatic alcohols and aldehydes of the honey bee cocoon induce arrestment behavior in Varroa jacobsoni (Acari: Mesostigmata), an ectoparasite of Apis mellifera. Arch Insect Biochem Physiol 37:129–145CrossRefGoogle Scholar
  6. Elzen PJ, Westervelt D (2002) Detection of coumaphos resistance in Varroa destructor in Florida. Am Bee J 142:291–292Google Scholar
  7. Elzen PJ, Baxter JR, Spivak M, Wilson WT (1999) Amitraz resistance in Varroa: a new discovery in North America. Am Bee J 139:362Google Scholar
  8. Fries I, Wallner K, Rosenkranz P (1998) Effects on Varroa jacobsoni from acaricides in beeswax. J Apic Res 37:85–90Google Scholar
  9. Harbo JR, Harris JW (2001) The relationship between nonreproduction of varroa and the quantity of worker brood. Am Bee J 141:889–890Google Scholar
  10. Harbo JR, Harris JW (2005) Suppressed mite reproduction explained by the behaviour of adult bees. J Apic Res 44:21–23Google Scholar
  11. Harbo J, Hoopingarner RA (1997) Honey bees (Hymenoptera: Apidae) in the United States that express resistance to Varroa jacobsoni. J Econ Entomol 90:893–898Google Scholar
  12. Hepburn HR, Kurstjens SP (1988) The combs of honey bees as composite materials. Apidologie 19:25–36CrossRefGoogle Scholar
  13. Jiménez JJ, Bernal JL, del Nozal MJ, Martín MT (2005) Residues of organic contaminants in beeswax. Eur J Lipid Sci Technol 107:896–902CrossRefGoogle Scholar
  14. Kochansky J, Wilzer K, Feldlaufer M (2001) Comparison of the transfer of coumaphos from beeswax into syrup and honey. Apidologie 32:119–125CrossRefGoogle Scholar
  15. Kraus B, Page RE Jr (1995) Population growth of Varroa jacobsoni Oud in Mediterranean climates of California. Apidologie 26:149–157CrossRefGoogle Scholar
  16. Leníček J, Sekyra M, Novotná AR, Vášová E, Titěra D, Veselý V (2006) Solid phase microextraction and gas chromatography with ion trap detector (GC-ITD) analysis of amitraz residues in beeswax after hydrolysis to 2,4-dimethylaniline. Anal Chim Acta 571:40–44PubMedCrossRefGoogle Scholar
  17. Lodesani M, Pellacani A, Bergomi S, Carpana E, Rabitti T, Lasagni P (1992) Residue determination for some products used against Varroa infestations in bees. Apidologie 23:257–272CrossRefGoogle Scholar
  18. Lodesani M, Costa C, Bigliardi M, Colombo R (2003) Acaricide residues in bee wax and organic beekeeping. Apiacta 38:31–33Google Scholar
  19. Martel A, Zeggane S (2002) Determination of acaricides in honey by high-performance liquid chromatography with photodiode array detection. J Chromatogr 954:173–180CrossRefGoogle Scholar
  20. Message D, Goncalves LS (1995) Effect of the size of worker brood cells of Africanized honey bees on infestation and reproduction of the ectoparasitic mite Varroa jacobsoni Oud. Apidologie 26:381–386CrossRefGoogle Scholar
  21. Nazzi F, Milani N, Vedova GD, Nimis M (2001) Semiochemicals from larval food affect the locomotory behaviour of Varroa destructor. Apidologie 32:149–155CrossRefGoogle Scholar
  22. Piccirillo GA, de Jong D (2004) Old honey bee brood combs are more infested by the mite Varroa destructor than are new brood combs. Apidologie 35:359–364CrossRefGoogle Scholar
  23. Rinderer TE, Baxter J (1978) Effect of empty comb on hoarding behavior and honey production of the honey bee. J Econ Entomol 71:757–759Google Scholar
  24. Rinderer TE, de Guzman LI, Harris J, Kuznetsov V, Delatte GT, Stelzer JA, Beaman L (2000) The release of ARS Russian honey bees. Am Bee J 140:305–307Google Scholar
  25. Rinderer TE, de Guzman LI, Delatte GT, Stelzer JA, Kuznetsov V, Beaman L, Watts R, Harris J (2001) Resistance to the parasitic mite Varroa destructor in honey bees from Far-eastern Russia. Apidologie 32:381–394CrossRefGoogle Scholar
  26. SAS Institute Inc (2001) SAS user’s guide, version 8.2. SAS Institute, Cary, NCGoogle Scholar

Copyright information

© US Government 2008

Authors and Affiliations

  • Lilia I. de Guzman
    • 1
  • Thomas E. Rinderer
    • 1
  • Amanda M. Frake
    • 1
  1. 1.USDA/ARSHoney Bee Breeding, Genetics and Physiology LaboratoryBaton RougeUSA

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