The Molecular and Biological Analysis of Ixodid Ticks Histamine Release Factors
We previously described a Dermacentor varibialis (DV) cDNA that encodes a ubiquitously expressed and tick saliva-secreted functional histamine release factor (HRF) homolog. In this study gene specific primers based on DVHRF open reading frame nucleotide sequence were utilized to amplify three orthologs, from the wood tick, D. andersoni (DA), the black legged tick, the southern cattle tick, Boophilus microplus (BM) and the lone star tick, Amblyomma americanum (AA). At nucleotide level, sequence comparisons revealed 98 89 and 84% similarity to DVHRF for DAHRF, AAHRF and BMHRF, respectively, while predicted polypeptide comparisons revealed 98, 96 and 91% similarity for DAHRF, AAHRF and BMHRF respectively. Phylogenetically, the tick HRF clade, while distinct (100% bootstrap value), is closely related to other arthropods, but distantly related to vertebrate and protozoan clades. Consistent with sequence similarity analysis, a DVHRF-specific northern blotting probe hybridized a ~900 base pair (bp) mRNA band on all RNA blots. Likewise a mouse polyclonal antibody to E. coli-expressed recombinant (r) DVHRF, cross-reacted baculovirus-expressed non-fusion rAAHRF, rDAHRF, and rBMHRF. As revealed by northern blotting analysis of larvae and nymph RNA, DVHRF mRNA is expressed in both immature and mature ticks indicating that its transcription is not developmentally regulated. Unlike rHRF/TCTP proteins of other organisms, the calcium-binding function may not be conserved for tick HRF homologs as revealed by the 45CaCl2+ overlay assay. Apparent global expression of DVHRF and its orthologs make this protein family an ideal target antigen for development of novel tick control strategies targeting multiple tick species.
KeywordsAnti-tick vaccine development Global expression Histamine release factor Ixodid ticks
Unable to display preview. Download preview PDF.
- Arcuri, F., Papa, S., Carducci, A., Romagnoli, R., Liberatori, S., Riparbelli, G.M., Sanchez, J.C., Tosi, P., Vecchio, M.T. 2004Translationally controlled tumor protein (TCTP) in the human prostate and prostate cancer cells: expression, distribution, and calcium binding activityProstate60130140CrossRefPubMedGoogle Scholar
- Cans, C., Passet, B.J., Shalak, V., Portebois, V.N., Crible, V., Amzallag, N., Allanic, D., Tufino, R., Argentin, M., Moras, D., Fiucci, G., Goud, B., Mirande, M., Amson, R., Telerman, A. 2003Translationally controlled tumor protein acts as a guanine nucleotide dissociation inhibitor on the translation elongation factor eEF1AProc. Natl. Acad. Sci. USA1001389213897PubMedGoogle Scholar
- Ermolayev, V., Weschke, W., Manteuffel, R. 2003Comparison of Al-induced gene expression in sensitive and tolerant soybean cultivarsJ. Exp. Botany5427452756Google Scholar
- Gnanasekar, M., Rao, K.V.N., Chen, L., Narayanan, R.B., Geetha, M., Scott, A.L., Ramaswamy, K., Kaliraji, P. 2002Molecular characterization of a calcium binding translationally controlled tumor protein homologue from the filarial parasites Brugia malayi and Wuchereria bancroftiMol. Biochem. Parasitol.121107118CrossRefPubMedGoogle Scholar
- Kemp, D.H., Bourne, A. 1980Boophilus microplus: the effect of histamine on the attachment of cattle-larvaestudies in vivo and in vitroJ. Parasitol.80487496Google Scholar
- Lee, M.J., Kusakabe, T., Kawaguchi, Y., Miyagawa, Y., Takahashi, M., Mon, H., Nho, S., Koga, K. 2004Molecular cloning and characterization of the translationally controlled tumor protein gene in Bombyx moriComp. Biochem. Physiol. Part B1393543Google Scholar
- McDonald, S.M., Bhisutthibhan, J., Shapiro, T.A., Rogerson, S.J., Taylor, T.E., Tembo, M., Langdon, J.M., Meshnick, S.R. 2001Immune mimicry in mamalaria: Plasmodium falciparum secretes a functional histamine-releasing factor homologue in vitro and in vivoProc. Natl. Acad. Sci. USA981082910832Google Scholar
- McDonald, S.M., Rafnar, T., Langdon, J., Lichtenstein, L.M. 1995Molecular identification of an Ige-dependent histamine-releasing factorScience269688690Google Scholar
- Paessen, G.C., Adams, P.L., Harlos, K., Nuttal, P.A., Stuart, D.I. 1999Tick histamine binding proteins: isolation, cloning, and three-dimensional structureMol. Cell3661671Google Scholar
- Stierum, R., Gaspari, M., Dommels, Y., Ouatas, T., Pluk, H., Jespersen, S., Vogels, J., Verheckx, K., Groten, J., Ommen, B. 2003Proteome analysis reveals protein associated with proliferation and differentiation of colorectal cancer cell line Caco-2Biochem. Biophys.16507391Google Scholar
- Vercouuter-Eduart, A.S., Czeszak, X., Crepin, M., Lemoine, J., Boilly, B., Bourhis, X., Peyrat, J.P., Hondermarck, H. 2001Proteomic detection of changes in protein synthesis induced by fibroblast growth factor-2 in MCF-7 human breast cancer cellsExp. Cell Res.2625968Google Scholar