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Rhodopirellula heiligendammensis sp. nov., Rhodopirellula pilleata sp. nov., and Rhodopirellula solitaria sp. nov. isolated from natural or artificial marine surfaces in Northern Germany and California, USA, and emended description of the genus Rhodopirellula

  • Nicolai Kallscheuer
  • Sandra Wiegand
  • Mareike Jogler
  • Christian Boedeker
  • Stijn H. Peeters
  • Patrick Rast
  • Anja Heuer
  • Mike S. M. Jetten
  • Manfred Rohde
  • Christian JoglerEmail author
Original Paper
  • 32 Downloads

Abstract

Expanding the collection of Planctomycetes by characterisation of novel species is key to better understanding of their complex lifestyles, uncommon cell biology and unexplored metabolism. Here, we isolated three novel planctomycetal strains from a kelp forest on the California Coastline at Monterey Bay or from plastic surfaces submerged in the Baltic Sea and the estuary of the river Warnow in the northeast of Germany. According to our phylogenetic analysis, the isolated strains Poly21T, Pla100T and CA85T represent three novel species within the genus Rhodopirellula. All three show typical planctomycetal traits such as division by budding. All are aerobic, mesophilic chemoheterotrophs and show genomic features comparable to other described Rhodopirellula species. However, strain CA85T is exceptional as it forms cream colonies, but no aggregates, which is a notable deviation from the pink- to red-pigmented and aggregate-forming Rhodopirellula species known thus far. We propose the names Rhodopirellula heiligendammensis sp. nov., Rhodopirellula pilleata sp. nov., and Rhodopirellula solitaria sp. nov. for the novel strains Poly21T (DSM 102266T = LMG 29467T = CECT 9847T = VKM B-3435T), Pla100T (DSM 102937T = LMG 29465T) and CA85T (DSM 109595T = LMG 29699T = VKM B-3451T), respectively, which we present as the respective type strains of these novel species.

Keywords

Marine bacteria Planctomycetes Rhodopirellula Baltic Sea Kelp forest Microplastic particles Monterey Bay 

Notes

Acknowledgements

Part of this research was funded by the Deutsche Forschungsgemeinschaft Grants KA 4967/1-1 and JO 893/4-1, Grant ALWOP.308 of the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO), SIAM (Soehngen Institute for Anaerobic Microbiology) Grant No. 024002002 and the Radboud Excellence fellowship. We thank Ina Schleicher for skillful technical assistance. Brian Tindall and Regine Fähnrich from the DSMZ as well as the BCCM/LMG Bacteria collection we thank for support during strain deposition. We thank Anne-Kristin Kaster (KIT Karlsruhe, Germany) and Alfred M. Spormann (Stanford, USA) as well as the Aquarius Dive Shop Monterey and the Hopkins Marine Station for sampling support. We also thank our collaborators Sonja Oberbeckmann and Matthias Labrenz (IOW Warnemünde, Germany) for support during sampling.

Author contributions

NK wrote the manuscript and analysed the cultivation data. SW performed the genomic and phylogenetic analysis. AH, PR and MJ isolated the strains and performed the initial cultivation and strain deposition. SHP and CB performed the light microscopic analysis and prepared the LM pictures. MSMJ contributed to text preparation and revised the manuscript. MR performed the electron microscopic analysis and prepared the SEM pictures. CJ took the samples and supervised the study. All authors read and approved the final version of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This article does not contain any studies with animals performed by any of the authors.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Nicolai Kallscheuer
    • 1
  • Sandra Wiegand
    • 1
  • Mareike Jogler
    • 1
    • 2
  • Christian Boedeker
    • 2
  • Stijn H. Peeters
    • 1
  • Patrick Rast
    • 2
  • Anja Heuer
    • 2
  • Mike S. M. Jetten
    • 1
  • Manfred Rohde
    • 3
  • Christian Jogler
    • 1
    Email author
  1. 1.Department of MicrobiologyRadboud UniversityNijmegenThe Netherlands
  2. 2.Leibniz Institute DSMZBrunswickGermany
  3. 3.Helmholtz Centre for Infection ResearchBrunswickGermany

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