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Pseudomonas khazarica sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from Khazar Sea sediments

  • Vahideh Tarhriz
  • Imen Nouioui
  • Cathrin Spröer
  • Susanne Verbarg
  • Vida Ebrahimi
  • Carlos Cortés-Albayay
  • Peter Schumann
  • Mohammad Amin Hejazi
  • Hans-Peter KlenkEmail author
  • Mohammad Saeid HejaziEmail author
Original Paper

Abstract

A novel Gram-negative, aerobic, motile and rod-shaped bacterium with the potential to biodegrade polycyclic aromatic hydrocarbons, was isolated from Khazar (Caspian) Sea. Strain TBZ2T grows in the absence of NaCl and tolerates up to 8.5% NaCl. Growth occurred at pH 3.0–10.0 (optimum, pH 6.0–7.0) and 10–45 °C (optimum, 30 °C). The major fatty acids are C18:1ω7C, C16:1ω7C/ C15:0 iso 2-OH, C16:0, C12:0, C10:0 3-OH, C12:0 3-OH. The major polar lipids include diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and the predominant respiratory quinone is ubiquinone Q-9. The 16S rRNA gene sequence analysis showed that strain TBZ2T is a member of the genus Pseudomonas with the highest similarity to P. oleovorans subsp. oleovorans DSM 1045T (98.83%), P. mendocina NBRC 14162T (98.63%), P. oleovorans subsp. lubricantis RS1T (98.61%) and P. alcaliphila JCM 10630T (98.49%) based on EzBioCloud server. Phylogenetic analyses using housekeeping genes (16S rRNA, rpoD, gyrB and rpoB) and genome sequences demonstrated that the strain TBZ2T formed a distinct branch closely related to the type strains of P. mendocina and P. guguanensis. Digital DNA-DNA hybridisation and average nucleotide identity values between strain TBZ2T and its closest relatives, P. mendocina NBRC 14162T (25.3%, 81.5%) and P. guguanensis JCM 18146T (26.8%, 79.0%), rate well below the designed threshold for assigning prokaryotic strains to the same species. On the basis of phenotypic, chemotaxonomic, genomic and phylogenetic results, it is recommended that strain TBZ2T is a novel species of the genus Pseudomonas, for which the name Pseudomonas khazarica sp. nov., is proposed. The type strain is TBZ2T (= LMG 29674T = KCTC 52410T).

Keywords

Pseudomonas khazarica Polycyclic aromatic hydrocarbons Khazar (Caspian) sea 

Notes

Acknowledgements

This work was supported by the Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. We also acknowledge School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK. Genome sequencing was provided by MicrobesNG (https://www.microbesng.uk), which is supported by the BBSRC (Grant Number BB/L024209/1).

Author contributions

VT isolated the bacterium; CS, SV and PS did fatty acids, 16S analysis and description; VT and VE did phenotypic, biochemical and genotypic experiments; IN performed the phylogenetic and genotypic analyses and CC-A analysed the genome and provided the genome features; MAH and VT prepared the manuscript; HPK and MSH wrote and edited the manuscript; VT, HPK and MSH designed the experiments; HPK and MSH led and supervised the project.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no direct or indirect conflict of interest.

Ethical approval

It is the original work of the authors. The work described has not been submitted elsewhere for publication, in whole or in part, and all authors listed carry out the data analysis and manuscript writing and “This article does not contain any studies with human participants or animals performed by any of the authors”. Moreover, all authors read and approved the final manuscript.

Supplementary material

10482_2019_1361_MOESM1_ESM.docx (1.3 mb)
Supplementary file1 (DOCX 1353 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Vahideh Tarhriz
    • 1
  • Imen Nouioui
    • 2
  • Cathrin Spröer
    • 3
  • Susanne Verbarg
    • 3
  • Vida Ebrahimi
    • 4
  • Carlos Cortés-Albayay
    • 2
  • Peter Schumann
    • 3
  • Mohammad Amin Hejazi
    • 5
  • Hans-Peter Klenk
    • 2
    Email author
  • Mohammad Saeid Hejazi
    • 1
    • 4
    • 6
    Email author
  1. 1.Molecular Medicine Research Center, Biomedicine InstituteTabriz University of Medical SciencesTabrizIran
  2. 2.School of Natural and Environmental SciencesNewcastle UniversityNewcastle upon TyneUK
  3. 3.Leibniz Institute DSMZ-German Collection of Microorganisms and Cell CulturesBrunswickGermany
  4. 4.Faculty of PharmacyTabriz University of Medical SciencesTabrizIran
  5. 5.Branch for the Northwest and West RegionAgriculture Biotechnology Research Institute of Iran (ABRII)TabrizIran
  6. 6.School of Advanced Biomedical SciencesTabriz University of Medical SciencesTabrizIran

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