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Halobellus captivus sp. nov., an extremely halophilic archaeon isolated from a subterranean salt mine

  • Shaoxing ChenEmail author
  • Siqi Sun
  • Yao Xu
  • Feilong Chen
  • Jingwen Liu
Original Paper

Abstract

An extremely halophilic archaeon, strain ZY21T, was isolated from a subterranean rock salt sample in Yunnan, China. Colonies of strain ZY21T on nutrient-rich agar plates are orange, wet and transparent. Cells are pleomorphic, motile, Gram-stain negative and lyse in distilled water. Cells can grow at 20–55 °C (optimum 42 °C), in the presence of 15–30% (w/v) NaCl (optimum 18–20%) and at pH 6.0–9.5 (optimum 7.5). Mg2+ is required for growth (optimum 0.3 M). The major polar lipids of strain ZY21T are phosphatidylglycerol, phosphatidylglycerol sulfate and phosphatidylglycerol phosphate methyl ester, sulfated mannosyl-glucosyl-glycerol diether-1 and seven unidentified glycolipids. Sequence similarity searches with the 16S rRNA gene and rpoB′ gene showed that strain ZY21T is closely related to Halobellus rufus CBA1103T (sequence similarities: 97.5% for 16S rRNA gene and 93.3% for rpoB′ gene). The DNA G+C content of strain ZY21T was determined to be 63.0 mol% based on the draft genome sequence. Genome-based sequence similarity analysis showed that the values of the ANI, AAI, and DDH were far below the boundary for delineation of new species. Phenotypic, chemotaxonomic characteristics and phylogenetic properties suggest that strain ZY21T represents a novel species in the genus Halobellus, for which the name Halobellus captivus sp. nov. is proposed. The type strain is ZY21T (= CGMCC 1.16343T = NBRC 113439T).

Keywords

Haloarchaea Halobellus Salt mine Salt deposit Polyphasic taxonomy 

Abbreviations

ANI

Average nucleotide identity

AAI

Average amino-acid identity

DDH

DNA–DNA hybridization

CGMCC

China General Microbiological Culture Collection Center

NBRC

NTE Biological Resource Center (Japanese)

MES

2-Morpholinoethanesulfonic acid

PIPES

1,4-Piperazine bis (ethanesulfonic acid)

CHES

2-(Cyclohexylamino) ethanesulfonic acid

CAPS

N-Cyclohexyl-3-aminopropanesulfonic acid

DMSO

Dimethylsulfoxide

OD

Optical density

DDH

DNA–DNA hybridization

TLC

Thin-layer chromatography

Notes

Acknowledgements

We thank Professor Zhu L. Yang from the Kunming Institute of Botany, Chinese Academy of Sciences for the help in sample collection, and we also thank Dr. Hao Yan from the College of Life Sciences, Anhui Normal University, for his technical assistance.

Author contributions

SC conceived the project, analysed the data, and drafted the manuscript. SS, YX, FC, and JL performed the experiment. SS and YX critically revised the manuscript. All authors read and approved the final manuscript.

Funding

This work was supported by grants from the National Natural Science Foundation of China (31460003), the Anhui Provincial Key Lab. of the Conservation and Exploitation of Biological Resources (591601), the China Scholarship Council (201808340054) and the Department of Education Anhui Province, China.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

No specific ethical or institutional permits were required to conduct sampling and the experimental studies did not involve endangered or protected species.

Supplementary material

10482_2019_1332_MOESM1_ESM.docx (2.2 mb)
Supplementary file1 (DOCX 2276 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.College of Life SciencesAnhui Normal UniversityWuhuPeople’s Republic of China
  2. 2.College of Life SciencesHonghe UniversityMengziPeople’s Republic of China

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