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Antonie van Leeuwenhoek

, Volume 112, Issue 12, pp 1827–1839 | Cite as

Isolation, functional characterization and efficacy of biofilm-forming rhizobacteria under abiotic stress conditions

  • Firoz Ahmad Ansari
  • Iqbal AhmadEmail author
Original Paper

Abstract

Abiotic stresses such as salinity, drought and excessive heat are associated with significant loss of crop productivity globally, and require effective strategies for their reduction or tolerance. Biofilm-forming rhizobacteria, which harbor multifarious plant growth promoting traits and tolerance to abiotic stress, are believed to benefit plant health and production even under environmental stresses. The primary objective of this study was to investigate indigenous biofilm-forming rhizobacteria (Pseudomonas spp., Bacillus sp., Pantoea sp., Brevibacterium sp. and Acinetobacter sp.) for their functional diversity relevant to plant growth promoting activities, biofilm development and tolerance to abiotic stress conditions. The most promising isolates among FAP1, FAP2, FAP3, FAP4, FAP5, FAP10, FAB1, FAB3 and FAA1 were selected. Rhizobacteria exhibited high tolerance to salinity (1.5 M NaCl) and drought stress (up to 55% PEG-6000) conditions in vitro. The isolates demonstrated varying levels of PGP activities (IAA production and phosphate solubilization), biofilm development, and production of alginate and exopolysaccharides in the presence of salinity, drought stress and elevated temperature. Further efficacy of the isolates was demonstrated by inoculating to wheat (Triticum aestivum L.) plants in greenhouse conditions under both normal and drought stress for up to 30 days inoculation. The plant growth potential of the isolates was in the order of FAP3 > FAB3 > FAB1 > FAP10 > FAP5 > FAP4 > FAA1 > FAP2 > FAP1. The present study resulted in successful selection of promising PGPR as identified by 16S rRNA gene sequence analysis. Field study is needed to evaluate their relative performance in both ‘normal’ and stressed environments in order to be exploited for plant stress management.

Keywords

Abiotic stress Biofilm Isolation PCR Plant growth Rhizosphere 

Notes

Acknowledgements

The authors gratefully acknowledge the University Grant Commission (UGC), New Delhi, for research support as a research fellowship (MAN-SRF). We also acknowledge the support of the University Sophisticated Instrumentation Facility (USIF), Aligarh Muslim University, Aligarh and Macrogen Sequencing Team, Seoul, South Korea, for providing instrumentation facilities. We are highly thankful to Prof. John Pichtel, Ball State University, USA, for critical review and language editing of the manuscript.

Author’s contribution

The authors, F.A. Ansari and I. Ahmad designed the experiment and F.A. Ansari performed the experiments and data analysis. F.A. Ansari wrote the manuscript, and I. Ahmad edited the manuscript critically and very carefully. All authors have read the manuscript and approved the data of the manuscript in its present form.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Biofilm Research Lab, Department of Agricultural Microbiology, Faculty of Agricultural SciencesAligarh Muslim UniversityAligarhIndia

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