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Antonie van Leeuwenhoek

, Volume 112, Issue 12, pp 1815–1826 | Cite as

Putative virulence factors of Plesiomonas shigelloides

  • Melissa S. Edwards
  • Richard William McLaughlin
  • Jia Li
  • XiaoLing Wan
  • Yingli Liu
  • HaiXia Xie
  • YuJiang Hao
  • JinSong ZhengEmail author
Original Paper

Abstract

Plesiomonas shigelloides is a Gram-negative rod-shaped bacterium which has been isolated from humans, animals and the environment. It has been associated with diarrhoeal disease in humans and various epizootic diseases in animals. In this study P. shigelloides strains were isolated from the faecal material of a captive Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis; YFP) living in semi-natural conditions in China. Plesiomonas shigelloides strain EE2 was subjected to whole genome sequencing. The draft genome was then compared to the genome sequences of ten other P. shigelloides isolates using the Pathosystems Resource Integration Center pipeline. In addition to several virulence factors which have been previously reported, we are proposing new candidate virulence factors such as a repeats-in-toxin protein, lysophospholipase, a twin-arginine translocation system and the type VI secretion effector Phospholipase A1.

Keywords

Plesiomonas shigelloides Draft genome Virulence factors Neophocaena asiaeorientalis asiaeorientalis PATRIC 

Notes

Acknowledgments

The authors thank Rebecca Wattam at Virginia Tech for her assistance on the use of PATRIC. This research was done as part of a Provost Honors project under the leadership of Zina Haywood, Executive Vice President/Provost. We thank Jennifer Cumpston and Donald Zakutansky for their enthusiastic support of this research. We also thank the staff of the Tian-e-Zhou National Natural Reserve for their help in collecting faecal samples. This work was supported by Grants from the Bureau of Science and Technology for Development, Chinese Academy of Sciences (No. ZSSD-004); the National Natural Science Foundation of China (Nos. 31430080, 31870372); and the Special Conservation Fund for the Yangtze finless porpoise from the Ministry of Agriculture of China.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10482_2019_1303_MOESM1_ESM.doc (1.6 mb)
Supplementary material 1 (DOC 1620 kb)

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.General StudiesGateway Technical CollegeKenoshaUSA
  2. 2.The Key Laboratory of Aquatic Biodiversity and Conservation of the Chinese Academy of Sciences, Institute of HydrobiologyChinese Academy of SciencesWuhanChina
  3. 3.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiologyChinese Academy of SciencesWuhanChina
  4. 4.University of Chinese Academy of SciencesBeijingChina

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