Mucilaginibacter endophyticus sp. nov., an endophytic polysaccharide-producing bacterium isolated from a stem of Miscanthus sinensis

  • Zhendong Zhang
  • Feng Sun
  • Yan Chen
  • Lunguang Yao
  • Zhaojin ChenEmail author
  • Wei TianEmail author
Original Paper


In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%). The DNA G + C content of strain RS1T was determined to be 42.80 mol%. ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species were less than 95% and 70%, respectively. The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH). The only isoprenoid quinone detected was MK-7. Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp. nov. is proposed (type strain RS1T = KCTC 62785T = GDMCC 1.1414T).


Mucilaginibacter endophyticus Endophytic bactium Molecular phylogeny Polyphasic taxonomy 



This research was supported by National Natural Science Foundation of China (Grant No. 41601332), the Key Research Project of Colleges and Universities of Henan Province Education Department (Grant No. 16A210012), the Key Scientific and Technological Project of Henan Province (Grant No. 172102110259) and the Youth Science Foundation of Gansu Province (18JR3RA023).

Author Contribution’s

Zhang ZD: He supported the procedures and methods for the characterization for the strain RS1T and the closest type strains. He also made the tables and figure and wrote the manuscript. He had prepared the lyophilized powder of strain RS1T and the closest related strains for analysis of polar lipids and isoprenoid quinone. Sun F: He had collected the samples for isolation of the strain RS1T, and isolated bacteria from the samples, and purified the strains. He was in charge of analysis of polar lipids, and isoprenoid quinone, and transmission electron micrograph of the strain. Chen Y: She had collected the samples, and conducted the PCR and sequencing of the 16S rRNA gene. Yao LG: He had constructed the phylogenetic trees, and Gram staining of the cells and determined the cellular fatty acids. Chen ZJ: He proposed strain RS1T as a new species. He had conducted the experiments for bacterial phenotypic characteristics using the API strips. He support the funding for the study. Tian W: He had performed the comparison of the genomes of RS1T and the closet related type strains. The Latin name was proposed by Wei Tian.

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

10482_2019_1242_MOESM1_ESM.docx (1.7 mb)
Supplementary material 1 (DOCX 1720 kb)


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Northwest Hubei Research Institute of Traditional Fermented Food, College of Food Science and TechnologyHubei University of Arts and ScienceXiangyangPeople’s Republic of China
  2. 2.Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province, School of Agricultural EngineeringNanyang Normal UniversityNanyangPeople’s Republic of China
  3. 3.Nanjing Institute of Environmental SciencesMinistry of Environmental ProtectionNanjingPeople’s Republic of China

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