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Hymenobacter persicinus sp. nov., a novel member of the family Hymenobacteraceae

  • Leonid N. Ten
  • Na Yeong Jeon
  • Weilan Li
  • Myung Kyum Kim
  • Young-Je Cho
  • Hee-Young JungEmail author
Original Paper
  • 25 Downloads

Abstract

A bacterial strain, 1-3-3-3T, was isolated from a soil sample collected in Jeollabuk-do province, South Korea. Cells were observed to be Gram-stain negative, short rod-shaped and colonies to be red-pink in colour. Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Hymenobacter in the family Hymenobacteraceae, with high levels of 16S rRNA sequence similarity with Hymenobacter algoricola VUG-A23aT (98.0%), Hymenobacter knuensis 16F7C-2 (97.9%), Hymenobacter fastidiosus VUG-A124T (97.1%), Hymenobacter elongatus VUG-A112T (97.0%), Hymenobacter chitinivorans Txc1T (97.0%) and Hymenobacter aquaticus 16F3PT (96.7%). Growth of strain 1-3-3-3T was observed at 10–30 °C, pH 6–8 and in the presence of 0–1.0% NaCl. The genomic G + C content was determined to be 61.6 mol %. The predominant respiratory quinone of the isolate was found to be MK-7; the major fatty acids were identified as iso-C15:0 (19.9%), summed feature 3 (C16:1ω7c/C16:1ω6c, 19.7%), summed feature 4 (iso-C17:1 I/anteiso-C17:1 B, 17.8%), C16:1ω5c (12.5%) and anteiso-C15:0 (11.2%), and the major polar lipid was found to be phosphatidylethanolamine. The phenotypic and chemotaxonomic data support the affiliation of strain 1-3-3-3T with the genus Hymenobacter. However, the DNA-DNA relatedness between the isolate and its closest phylogenetic neighbours was lower than 34%. The DNA–DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain 1-3-3-3T represents a novel species in the genus Hymenobacter, for which the name Hymenobacter persicinus sp. nov. is proposed. The type strain is 1-3-3-3T (= KCTC 52742T = JCM 32191T).

Keywords

Hymenobacter Hymenobacteraceae Polyphasic taxonomy Soil bacteria 

Notes

Acknowledgements

This work was supported by Brain Pool Program (Grant No. 2018H1D3A2065415) through the National Research Foundation (NRF) funded by the Ministry of Science and ICT, Republic of Korea.

Author’s contribution

Leonid N. Ten analysed the data and wrote the manuscript, Na Yeong Jeon made the original isolation and performed the phenotypic characterization, Weilan Li performed TEM imaging and conducted the fatty acid analysis, Myung Kyum Kim performed 16S rRNA phylogeny, Young-Je Cho performed the phylogenetic analysis and taxonomic analysis, Hee-Young Jung designed, planned the study and wrote the manuscript. All authors approved the final version of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10482_2019_1236_MOESM1_ESM.pptx (652 kb)
Transmission electron micrograph of strain 1-3-3-3T grown on R2A agar at 25 °C for 3 days. Bar, 1 µm (PPTX 652 kb)
10482_2019_1236_MOESM2_ESM.pptx (632 kb)
Two-dimensional thin-layer chromatography of the polar lipids of strain 1-3-3-3T. Chloroform/methanol/water (65:25:4, v/v/v) was used for the first dimension, followed by chloroform/acetic acid/methanol/water (80:15:12:4, v/v/v/v) for the second dimension. The following spray reagents were used for detection: 5% ethanolic molybdatophosphoric acid (for total lipids), ninhydrin (for aminolipids), molybdenum blue (for phospholipids), and α-naphthol-sulfuric acid reagent (for glycolipids). Abbreviations: APL1–APL3, unidentified aminophospholipids; AGL, an unidentified aminoglycolipid; L1–L2, unidentified lipids; PL, an unidentified phospholipid; PE, phosphatidylethanolamine (PPTX 632 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Leonid N. Ten
    • 1
  • Na Yeong Jeon
    • 1
  • Weilan Li
    • 1
  • Myung Kyum Kim
    • 2
  • Young-Je Cho
    • 3
  • Hee-Young Jung
    • 1
    • 4
    Email author
  1. 1.School of Applied BiosciencesKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Department of Bio and Environmental TechnologySeoul Women’s UniversitySeoulRepublic of Korea
  3. 3.School of Food Science and Biotechnology/Food and Bio-Industry Research InstituteKyungpook National UniversityDaeguRepublic of Korea
  4. 4.Institute of Plant MedicineKyungpook National UniversityDaeguRepublic of Korea

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