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Paracoccus indicus sp. nov., isolated from surface seawater in the Indian Ocean

  • Danqiu Lin
  • Sidong Zhu
  • Yong Chen
  • Yizhe Huang
  • Jifang YangEmail author
  • Jigang ChenEmail author
Original Paper
  • 12 Downloads

Abstract

Strain IO390502T, isolated from surface seawater in the Indian Ocean, was characterised using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain IO390502T belongs to the genus Paracoccus and is closely related to Paracoccus seriniphilus DSM 14827T (97.6%), followed by P. zeaxanthinifaciens JCM 21774T (97.5%), P. homiensis DSM 17862T (97.3%), P. marcusii DSM 11574T (97.2%), P. haeundaensis BC 74171T (97.0%) and P. carotinifaciens E-396T (97.0%). Cells are Gram-negative, aerobic, poly-β-hydroxybutyrate-accumulating, motile, rod-shaped, and forms creamy-white colonies. Optimal growth occurred at 25–30 °C, pH 5–8, and in the presence of 3–8% NaCl. The genome of strain IO390502T has a G+C content of 64.9 mol% and a 3.5 Mb chromosome. The in silico DNA–DNA hybridisation and average nucleotide identity values between strain IO390502T and the three closely related taxa, P. seriniphilus DSM 14827T, P. zeaxanthinifaciens JCM 21774T and P. homiensis DSM 17862T, are 19.6%, 21.9% and 20.6%, and 76.0%, 79.9% and 77.8%, respectively. Phosphatidylglycerol is the major lipid present, ubiquinone-10 (Q-10) is the sole isoprenoid quinone, and the major cellular fatty acid is C18:1ω7c. Based on data from phenotypic tests and genotypic differences between strain IO390502T and its close phylogenetic relatives, strain IO390502T represents a new species belonging to the genus Paracoccus, for which the name Paracoccus indicus sp. nov. is proposed. The type strain is IO390502T (= JCM 32637T = CCTCC AB 2018071T).

Keywords

Paracoccus Surface seawater Indian Ocean Phylogenetic analysis Cell morphology Classification 

Notes

Acknowledgements

This work was funded by the China Ocean Mineral Resources R&D Association (CPMRA) Foundation (Grant No. DY125-15-R-03), the Zhejiang Provincial Top Key Discipline of Biological Engineering (Grant No. CX2017020), and the Key Science and Technology Program of Ningbo City, Zhejiang Province, China (Grant No. 2012C10038).

Author contributions

Conceived and designed the experiments: Danqiu Lin, Jigang Chen, Jifang Yang. Performed the experiments: Danqiu Lin, Sidong Zhu, Yong Chen, Yizhe Huang. Analyzed the data: Danqiu Lin, Jigang Chen. Contributed reagents/materials: Jifang Yang. Wrote the paper: Danqiu Lin, Jigang Chen.

Compliance with ethical standards

Conflict of interest

All authors discussed and approved the final manuscript, and declare no competing interests.

Ethical statement

This article does not contain any studies with animals performed by any of the authors.

Supplementary material

10482_2019_1226_MOESM1_ESM.pdf (531 kb)
Supplementary material 1 (PDF 530 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.College of Biological and Environmental SciencesZhejiang Wanli UniversityNingboPeople’s Republic of China

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