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Winogradskyella algae sp. nov., a marine bacterium isolated from the brown alga

  • Valeriya V. Kurilenko
  • Lyudmila A. Romanenko
  • Marina P. Isaeva
  • Vassilii I. Svetashev
  • Valery V. Mikhailov
Original Paper
  • 28 Downloads

Abstract

An aerobic, Gram-negative, yellow-pigmented non-motile rod-shaped bacterium Kr9-9T was isolated from a brown alga specimen collected near the Kuril Islands. Based on the 16S rRNA gene sequence analysis strain Kr9-9T was assigned to the genus Winogradskyella, and its close phylogenetic neighbors were found to be Winogradskyella damuponensis KCTC 23552T, Winogradskyella sediminis LMG 28075T, and Winogradskyella rapida CCUG 59098T showing high similarities of 98.1%, 97.5%, and 97.1%, respectively. It contained MK-6 as the predominant menaquinone and iso-C15:0, anteiso-C15:0, iso-C16:0 3-OH followed by iso-C15:1 as the major fatty acids. Polar lipids included phosphatidylethanolamine, three unidentified aminolipids and an unidentified lipid. The DNA C+C content was 32.3 mol%. Based on the phylogenetic analysis and distinctive phenotypic characteristics, strain Kr9-9T is concluded to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella algae sp. nov. is proposed. The type strain of the species is strain Kr9-9T (= KMM 8180T = KACC 19709T).

Keywords

Winogradskyella algae sp. nov. Marine Brown alga 

Notes

Acknowledgements

We would like to thank Dr. Claudine Vereecke, BCCM™/LMG Bacteria Collection, Ghent University, Gent, Belgium, for providing the type strain Winogradskyella sediminis LMG 28075T for comparative analyses. This study was supported by a programme of “Bioresource collections”.

Author’s contributions

Author V.V. Kurilenko isolated novel strain, designed study and tested bacteria. Author L.A. Romanenko tested bacteria, analyzed data and wrote the first draft of the manuscript. Authors V.V. Kurilenko and M.P. Isaeva performed the molecular studies and literature searches. Author V.I. Svetashev carried out lipid analyses. Author V.V. Mikhailov managed the analyses of the study and analyzed the results. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

All the authors have declared no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10482_2018_1207_MOESM1_ESM.doc (1.5 mb)
Supplementary material 1 (DOC 1564 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Valeriya V. Kurilenko
    • 1
  • Lyudmila A. Romanenko
    • 1
  • Marina P. Isaeva
    • 1
  • Vassilii I. Svetashev
    • 2
  • Valery V. Mikhailov
    • 1
  1. 1.G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern BranchRussian Academy of SciencesVladivostokRussia
  2. 2.Zhirmunsky Institute of Marine Biology, Far Eastern BranchRussian Academy of SciencesVladivostokRussia

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