A novel Gram-stain negative, rod-shaped and motile bacterial strain, designated strain Seoho-38T, was isolated from a eutrophic lake in South Korea. Polyphasic taxonomic studies were performed to investigate the taxonomic position of the new isolate. The phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain Seoho-38T formed a distinct cluster with Nevskia ramosa Soe1T, Nevskia persephonica G6M-30T, Nevskia soli GR15-1T, Nevskia terrae KIS13-15T and Nevskia aquatilis F2-63T with bootstrap resampling value of 100%. Of those Nevskia strains, the new isolate shows high sequence similarity with N. ramosa Soe1T (98.7%) and N. persephonica G6M-30T (97.2%), and values lower than 96.5% with the other type strains. The new isolate was observed to grow aerobically in 0–1.5% (w/v) NaCl (optimum 0%), at pH 7.0–9.0 (optimum pH 7.0) and temperature 15–36 °C (optimum 20–30 °C) on R2A medium. DNA–DNA relatedness values between strain Seoho-38T and the type strains of reference species in the genus Nevskia were < 24%. The genomic DNA G + C content was determined to be 67.4 mol%. Ubiquinone-8 (Q-8) (95%) and ubiquinone-7 (Q-7) (5%) were identified as the respiratory quinones. The cellular polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a phosphoaminolipid, two glycolipids, an aminolipid and four unidentified lipids. The major fatty acid components were found to include summed feature 3 (C16:1ω7c and/or C16:1ω6c), summed feature 8 (C18:0ω7c and/or C18:0ω6c), C16:0 and C14:0. Based on the above polyphasic evidence, strain Seoho-38T (= KCTC 52221T = JCM 31888T) represents a new species of the genus Nevskia, for which the name Nevskia lacus sp. nov. is proposed.
Nevskia lacus sp. nov. NevskiaPolyphasic taxonomy Harmful cyanobacterial blooms
This is a preview of subscription content, log in to check access.
This research was supported by the Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) and Korea Research Fellowship program, funded by the Ministry of Science and ICT (2016M1A5A1027453 and 2015H1D3A1060001).
Y.C. analysed data, interpreted results and wrote the manuscript; S.J.C. and C.Y.A took samples; Y.C. and S.J.C. performed genetic, morphological, physiological and chemotaxonomical analysis. A.R.C helped to interpret data analysis. S.K.W, H.G.L, H.M.O. and C.Y.A. revised the manuscript. All authors reviewed and approved the manuscript.
Conflict of interest
The authors declare that they have no conflict of interest.
Cui Y, Baek SH, Wang L, Lee HG, Cui C, Lee ST, Im WT (2012) Streptomyces panacagri sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 62:780–785CrossRefGoogle Scholar
Dastager SG, Mawlankar R, Mual P, Verma A, Krishnamurthi S, Joseph N, Shouche YS (2015) Bacillus encimensis sp. nov. isolated from marine sediment. Int J Syst Evol Microbiol 65:1421–1425CrossRefGoogle Scholar
Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Evol Microbiol 39:224–229Google Scholar
Famintzin A (1892) Eine neue Bakterienform: Nevskia ramosa. Bull Acad Imp Sci St Petersb New Ser 2:481–486Google Scholar
Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376CrossRefGoogle Scholar
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucl Acids Symp Ser 41:95–98Google Scholar
Hiraishi A, Ueda Y, Ishihara J, Mori T (1996) Comparative lipoquinone analysis of influent sewage and activated sludge by highperformance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469CrossRefGoogle Scholar
Jin L, Lee HG, La HJ, Ko SR, Ahn CY, Oh HM (2014) Ferruginibacter profundus sp. nov., a novel member of the family Chitinophagaceae, isolated from freshwater sediment of a reservoir. Antonie Van Leeuwenhoek 106:319–323CrossRefGoogle Scholar
Jin L, Ko SR, Cui Y, Lee CS, Oh HM, Ahn CY, Lee HG (2017) Pusillimonas caeni sp. nov., isolated from a sludge sample of a biofilm reactor. Antonie Van Leeuwenhoek 110:125–132CrossRefGoogle Scholar
Kim SJ, Weon HY, Kim YS, Park IC, Son JA, Kwon SW (2011) Nevskia terrae sp. nov., isolated from soil. Int J Syst Evol Microbiol 61:1226–1229CrossRefGoogle Scholar
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721CrossRefGoogle Scholar
Kimura M (1984) The neutral theory of molecular evolution. Cambridge University Press, CambridgeGoogle Scholar
Lane D (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterialsystematics. Wiley, ChichesterGoogle Scholar
Leandro T, Franca L, Nobre MF, Schumann P, Rossello-Mora R, da Costa MS (2012) Nevskia aquatilis sp. nov. and Nevskia persephonica sp. nov., isolated from a mineral water aquifer and the emended description of the genus Nevskia. Syst Appl Microbiol 35:297–301CrossRefGoogle Scholar
Minnikin D, Patel P, Alshamaony L, Goodfellow M (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Evol Microbiol 27:104–117Google Scholar
Rosselló-Móra R, Trujillo ME, Sutcliffe IC (2017) Introducing a digital protologue: a timely move towards a database-driven systematics of archaea and bacteria. Antonie Van Leeuwenhoek 110:455–456CrossRefGoogle Scholar
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. MIDI Inc, NewarkGoogle Scholar
Sturmeyer H, Overmann J, Babenzien HD, Cypionka H (1998) Ecophysiological and phylogenetic studies of Nevskia ramosa in pure culture. Appl Environ Microbiol 64:1890–1894PubMedPubMedCentralGoogle Scholar
Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128CrossRefGoogle Scholar
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefGoogle Scholar
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882CrossRefGoogle Scholar
Weon HY, Kim BY, Son JA, Song MH, Kwon SW, Go SJ, Stackebrandt E (2008) Nevskia soli sp. nov., isolated from soil cultivated with Korean ginseng. Int J Syst Evol Microbiol 58:578–580CrossRefGoogle Scholar