Antonie van Leeuwenhoek

, Volume 112, Issue 5, pp 661–668 | Cite as

Phyllobacterium phragmitis sp. nov., an endophytic bacterium isolated from Phragmites australis rhizome in Kumtag Desert

  • Li-xiong Liang
  • Qi-wu Sun
  • Nan Hui
  • Xiao-xia Zhang
  • Lu-bin Li
  • Lei LiuEmail author
Original Paper


A Gram-negative rod, designated strain 1N-3T, was isolated from a rhizome of Phragmites australis grown in Kumtag Desert, China. Phylogenetic analysis showed that the strain is closely related to Phyllobacterium salinisoli LMG 30173T with 99.0% sequence similarity in the 16S rRNA gene and 92.9% in the atpD gene. Growth was observed at salinities of 0–4% (w/v), over a pH range of 5.0–10.0 (optimum 8.0) and at temperatures of 15–40 °C (optimum 30 °C). The predominant cellular fatty acids were identified as summed feature 8 (C18:1ω7c/C18:1ω6c). The G+C content of strain 1N-3T was determined to be 60.1%. Based on phenotypic, chemotaxonomic, phylogenetic properties and genomic comparison, it is concluded that strain 1N-3T represents a novel species of the genus Phyllobacterium, for which the name Phyllobacterium phragmitis sp. nov. is proposed. The type strain is 1N-3T (=KCTC 62183T =ACCC 60071T).


Phragmites australis Phyllobacterium Novel species Rhizome 



This work was supported by The State Key Research and Development Program of China (2016YFC0500801).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical statement

No specific ethical or institutional permits were required to conduct sampling and the experimental studies did not involve endangered or protected species.

Supplementary material

10482_2018_1195_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 19 kb)


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Li-xiong Liang
    • 1
  • Qi-wu Sun
    • 1
  • Nan Hui
    • 1
  • Xiao-xia Zhang
    • 2
  • Lu-bin Li
    • 1
  • Lei Liu
    • 1
    Email author
  1. 1.State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of ForestryChinese Academy of ForestryBeijingPeople’s Republic of China
  2. 2.Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

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