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Antonie van Leeuwenhoek

, Volume 112, Issue 4, pp 641–649 | Cite as

Mucilaginibacter xinganensis sp. nov., a phenanthrene-degrading bacterium isolated from wetland soil

  • Min-zhi Jiang
  • Zhi-jun Qiu
  • Guang-wu Zhang
  • Shi-yin Gao
  • Xiao-yan YouEmail author
  • Ying-fei MaEmail author
Original Paper
  • 66 Downloads

Abstract

An aerobic, Gram-stain negative, rod-shaped and non-motile strain, BJC16-A31T, was isolated from the wetland soil sample taken from Daxing’anling, Heilongjiang, People’s Republic of China. Strain BJC16-A31T was found to be oxidase- and catalase-positive, and produced light orange colonies on modified R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BJC16-A31T is closely related to Mucilaginibacter gotjawali SA3-7T with 96.54% sequence similarity and it formed a separate lineage in the genus Mucilaginibacter. Strain BJC16-A31T contained menaquinone-7 (MK-7) as the predominant isoprenoid quinine. Anteiso-C15:0, C16:0 and anteiso-C15:0 were the major fatty acids. The major polar lipids were phosphatidylethanolamine, six unidentified polar lipid, two unidentified aminophospholipids and one unidentified aminolipid. The genome is composed of a circular 5,301,339 bp chromosome with average G + C percentage of 42.25%. The Average Nucleotide Identity (ANI) between strain BJC16-A31T and M. gotjawali SA3-7T was 77.51%. Combined phenotypic, chemotaxonomic, phylogenetic and genomic characteristics support the conclusion that strain BJC16-A31T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter xinganensis sp. nov. is proposed. The type strain is BJC16-A31T (= CGMCC 1.12728T = NBRC 110384T).

Keywords

Mucilaginibacter Novel species Bacterium Genome 

Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (31200035), and SRTP program of Henan University of Science and Technology (2017130). We thank the lab of Professor Shuang-jiang Liu (Institute of Microbiology, Chinese Academy of Sciences, Beijing, China) for providing the soil samples. We also thank the Instrument Center of Institute of Microbiology for providing the transmission electron microscopy images.

Author contributions

Min-zhi Jiang performed the laboratory experiments and acquisition of data. Zhi-jun Qiu carried out the phylogenetic and bioinformatics analyses. Guang-wu Zhang performed the cellular fatty acid analyses. Shi-yin Gao prepared the genomic DNA. Xiao-yan You conceived the study and drafted the manuscript. Ying-fei Ma participated in the design of the study and contributed to the revisions. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical statement

This research was conducted in the absence of any ethical issue on animal research.

Supplementary material

10482_2018_1194_MOESM1_ESM.docx (1.2 mb)
Supplementary material 1 (DOCX 1196 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Luoyang Engineering and Technology Research Center of Microbial Fermentation, College of Food and BioengineeringHenan University of Science and TechnologyLuoyangPeople’s Republic of China
  2. 2.Center for Synthetic Biology Engineering Research, Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenPeople’s Republic of China
  3. 3.Luoyang Center for Disease Control and PreventionLuoyangPeople’s Republic of China

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