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Antonie van Leeuwenhoek

, Volume 112, Issue 4, pp 589–598 | Cite as

Yeast molecular chaperone gene SSB2 is involved in the endoplasmic reticulum stress response

  • Wei Zhao
  • Hong-Jing Cui
  • Kun-Pei Qiu
  • Tao Zhou
  • Xiao-Shan Hong
  • Xin-Guang LiuEmail author
Original Paper
  • 183 Downloads

Abstract

The Saccharomyces cerevisiae chaperone gene SSB2 belongs to the Hsp70 family. Unlike other HSP70 genes, SSB2 gene expression is reduced after heat shock. It has been reported that Ssb2p can be cross-linked to ribosome-bound nascent polypeptide chains, suggesting a potential role of SSB2 in the endoplasmic reticulum (ER) stress response. In this study, SSB2-deletion and SSB2-overexpression yeast strains were generated and applied to explore the potential mechanism by which SSB2 is involved in the tunicamycin (TM)-induced ER stress response. We demonstrate for the first time that SSB2 deficiency results in reduced resistance to TM, while overexpression of SSB2 increases resistance to TM in an IRE1-HAC1 pathway-dependent manner; these observations are related to changes in intracellular unfolded protein response activities (under the TM-stressed condition). Additionally, SSB2 deletion induces early apoptosis and it may play a causal role in the shortened replicative life span of ssb2Δ mutants observed in this study. These findings highlight the involvement of SSB2 in ER stress responses and ageing in yeast.

Keywords

ER stress Life span SSB2 Yeast 

Abbreviations

CLS

Chronological life span

ER

Endoplasmic reticulum

RLS

Replicative life span

S. cerevisiae

Saccharomyces cerevisiae

TM

Tunicamycin

UPR

Unfolded protein response

Notes

Acknowledgements

This work was supported by the China National Natural Science Foundation (31101051, 81671399), the Ordinary University Innovation Team Construction Project of Guangdong Province (2015KCXTD022). We are grateful to Brian K. Kennedy (Buck Institute), Matt Kaeberlein and Brian M. Wasko (University of Washington) for technical assistance.

Author's Contribution

X-GL designed the experiments. WZ, H-JC, K-PQ, TZ and X-SH performed experiments. WZ wrote the manuscript. All authors discussed the results and commented on the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10482_2018_1189_MOESM1_ESM.docx (113 kb)
Supplementary material 1 (DOCX 112 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Institute of Aging ResearchGuangdong Medical UniversityDongguanChina
  2. 2.Guangdong Provincial Key Laboratory of Medical Molecular DiagnosticsDongguanChina
  3. 3.Institute of GynecologyWomen and Children’s Hospital of Guangdong ProvinceGuangzhouChina
  4. 4.Institute of Biochemistry and Molecular BiologyGuangdong Medical UniversityDongguanChina

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